PubMed Health⌕ Search

Biomedical subjects

A Chandrasekharan

Publications and source records attributed to A Chandrasekharan.

11 recordsLinked to original sources
PubMed1989

Detection of Wuchereria bancrofti antigen in serum and finger prick blood samples by enzyme immunoassay: field evaluation.

We have recently reported that a monoclonal antibody-based enzyme immunoassay for detection of filarial antigen in human serum is sensitive and specific for active infection with Wuchereria bancrofti. The present studies were undertaken to assess the feasibility of testing whole blood collected by finger prick in this assay. A preliminary study was performed to compare antigen test results obtained with whole blood, blood dried on filter paper, and serum. Results obtained with anticoagulated whole blood specimens agrees with serum results in 94 of 97 cases. All whole blood and serum specimens from 28 people with positive microfilaria smears were positive in the test. Filter paper blood specimens were less satisfactory because of decreased sensitivity and specificity. A population-based survey of 1009 persons was conducted in a village near Calicut, India, to evaluate the use of finger prick blood specimens for filarial antigen detection in field studies. Thirty-eight of 39 microfilaria carriers had positive antigen tests. In addition, 10.7% of amicrofilaremic endemic controls had positive tests. Additional studies are needed to test the hypothesis that filarial antigenemia in endemic controls indicates the presence of subclinical infection.

Adolescent↗
PubMed1989

Epidemiology of brugian filariasis in a rural community of Kerala State.

The baseline clinico-parasitological parameters collected during the initial survey and the entomological observations made during the first year as a part of a longitudinal study undertaken in a brugian endemic rural community are presented in this paper. Out of the total (11.604) examined population, 8.1 per cent had microfilaraemia and 7.6 per cent had filarial disease. A highly significant (P less than 0.001) relationship of age was observed with microfilaraemia and the disease. The filarial disease rate showed no significant difference between two sexes while the microfilaria (Mf) rate was significantly (P less than 0.001) higher in males than females. A significant (P less than 0.001) relationship of microfilaraemia as well as of filarial disease was found with the income and the educational status of the individual. The mf and the disease rates did not differ by the family size except those families with 1-3 members. However, the relationship of family size was found to be significant (P less than 0.001) with disease and non-significant (P greater than 0.05) with microfilaraemia. The vectors were prevalent throughout the year but the infection was mostly confined to Mansonia annulifera.

Adolescent↗