A simple automatic kinetic enzyme immunoassay for digoxin.
Explore the source record for details and available documents.
Autoanalysis↗
Biomedical subjects
A Cid
Publications and source records attributed to A Cid.
Enzyme-linked immunosorbent choriomammotropin assay.
We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assayed 10 times on the same day, was 5.7%. The coefficient of variation for nine samples (3.5 to 9.0 mg/L) assayed on five different days was 7.9%. Forty-eight clinical samples were assayed (y) and compared with results obtained by radial immunodiffusion (x). The resulting regression equation was: y = 1.05x + 0.78; r = 0.91.
Enzyme-Linked Immunosorbent Assay↗
A new radioassay for serum vitamin B12 determination using chicken serum as a binder and an anion exchange resin column separation.
We present a new method for the determination of serum vitamin B12 concentration by competitive protein binding using 57Co labeled vitamin B12, chicken serum (CS) as the binding protein, and a sephadex column separation. We placed a mixture of extracted sample, labeled B12, and CS binder above an A-25 sephadex column, and after 1 hour incubation at room temperature drained the column and washed it twice with buffer. We counted the free fraction remaining in each column. Within run C.V.'s for low and high values in the normal range were 10.6% and 6.03%. The between run C.V.'s were 7.7% and 7.5% respectively. The values obtained for 95 clinical specimens correlated well with those for a reference method.
Animals↗
A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera.
A direct enzyme-linked immunoassay (ELISA), based on the "sandwich" principle on an antigen-coated plastic disc, was used for the rapid detection of rubella antibody. Results were obtained the same day, and the prior adsorption of sera to remove non-specific inhibitors was not necessary. The ELISA was compared to the hemagglutination-inhibition (HAI) test on 500 serum samples. There was general agreement between the two methods; most discrepancies occurred with low-titered HAI positive sera. There was excellent correlation between the tests with serum samples negative for rubella antibodies and those samples with HAI titers greater than or equal to 1:40.
Animals↗