Electrophoresis of semen on cellulose acetate strips allowed the localization of 19 protein bands. Twelve of these were analyzed for their composition and ten for their tissue of origin. Four bands were composed of prostatic products, three contained secretions form the seminal vesicles, and various serum products composed six others. Assays were also performed for acid phosphatase activity, glycoproteins and proteins bound to zinc. Proteins in semen were found to be broken down with time during incubation at 37C and therefore only fresh specimens or material which is frozen immediately after liquefaction may be used with these techniques. Our experiments clearly show that electrophoresis of semen may be a useful technique in clinical andrology: in particular in the case of excretory azoospermia and infections of the genital tract.

Acid Phosphatase↗

PubMedDate not supplied

Fructose, proteins and coagulation in human seminal plasma.

Fructose is produced and secreted by the seminal vesicles and it is believed to form tight bonds with certain proteins that are also of vesicular origin. Under normal conditions of liquefaction, fructose is totally dialyzable; however, blocking liquefaction by exposing semen to acidic conditions renders this sugar non dialyzable. Fructose therefore appears to play a role in the coagulation of semen. The formation of fructose-protein complexes may resolve physiological problems concerning osmotic pressure in the seminal vesicles: if all the fructose were present in free state, the osmotic pressure would be very high and incompatible with cell survival

Dialysis↗

PubMedDate not supplied

Semen parameters and capacitation.

A relationship exists between several semen parameters and the ability of spermatozoa to capacitate. The increase of oligoteratozoospermia, which could be related to testicular dysfunction, is correlated with the decrease of the percentage of spermatozoa able to "capacitate". The increase of loss of motility with time, which could be related to abnormalities of the seminal fluid, is also correlated with a decreased number of spermatozoa able to "capacitate".

Adult↗

PubMedDate not supplied

Comparative electrophoretic study between rabbit and human seminal fluid.

Cellulose acetate electrophoresis of rabbit seminal fluid give, after amidoschwartz staining, 19 protein bands, all migration towards the anode. Nine of them are present in at least 80% of the samples. With the acid of specific detection techniques, it was possible to demonstrate th presence of zinc in four bands, acid phosphatase activity in two and glycoprotein in three of the bands. Serum compounds seem to be present in small quantities. Study of fluids squeezed out from prostate, paraprostate, seminal vesicle and epididymal tail show few specific bands in these secretions: four in the case of prostate, three for paraprostate and one for seminal vesicle. Comparisons between human and rabbit seminal fluid electrophoresis show no relationship between these two types of secretion.

Acid Phosphatase↗

PubMedDate not supplied

On the glandular origin of seminal plasma lipids in man.

The aim of this study is to examine the glandular origin of seminal fluid lipids in man. The triglycerides, the total cholesterol, the non-esterified fatty acid and the total phospholipids were measured in seminal plasma of vasectomized patients (n = 8) and control subjects (n = 15). The same parameters were measured in seminal plasma collected in three fractions from split ejaculates (n = 10). The total cholesterol and the non-esterified fatty acid are principally prostatic in origin. The phospholipids are secreted by the epididymis but also by the prostate. The origin of the triglycerides seems to be very varied.

Cholesterol↗

PubMedDate not supplied

[Morphologic acrosomal changes in a man exposed to heat].

In view of a case of primary sterility with heat professional exposure and abuse of warm baths, we have studied the spermatozoa ultrastructural morphology. Same type of acrosomal malformations were observed in spermatozoa following experimental scrotal heating. These abnormalities appears to produce sterility in this case.

Acrosome↗

PubMedDate not supplied

[Value of a control of external quality of spermato-cytograms and perspectives].

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Forecasting↗