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A Comoy

Publications and source records attributed to A Comoy.

5 recordsLinked to original sources
PubMed1977

Suppression of generation of human cytotoxic effectors by lectins or lectin-activated peripheral blood lymphocytes.

The lectins phytohemagglutinin, pokeweed mitogen and concanavalin A used at their optimal mitogenic concentration, or human lymphocytes activated by the same mitogens, were found to suppress the in vitro generation of cytotoxic effectors when added to a cell-mediated lympholysis (CML) mixture during the first 48 h of culture. The data suggest that the suppressive mechanism is mediated to a greater extent by an allogeneic interaction between lectin-activated cells and the allogeneic cells present in the CML mixture than by suppressor cells induced by the lectin. Since partial suppression was observed with supernatants of activated lymphocytes cultured for 18 h with allogeneic stimulating cells (but not activated lymphocytes alone), a soluble mediator may be involved in the suppressive mechanism. The mechanism of suppression therefore may be identical to the preemption phenomenon recently described in primary and secondary CML.

Antigens↗
PubMed1976

A rapid HLA-D matching method using PHA blasts as responding cells (preliminary data on PHA blasts HLA-D typing).

Day 3-4 PHA stimulated lymphocytes were found to respond against HLA-D without any period of latency, thus allowing a discriminative MLR reading as early as 24-48 h. The test is therefore characterized by the minute number of responding cells necessary (5 X 10(3)), a one-way reaction without additional treatment of the stimualtion normal lymphocytes (5 x 10(4)), and the rapid obtaining of data. We found the preliminary data testing its concordance with classical HLA-D typing encouraging and worthy of being tested on a larger scale. Using mitogen stimulated recipient cells stored frozen, HLA-D matching with potential donors was found to be feasible within 24-48 h. The mechanism of such a prompt response by mitogen stimulated lymphocytes is unknown but appears to be independent of the nature and dose of a given mitogen.

HLA Antigens↗