Biomedical subjects

A Conchillo

Publications and source records attributed to A Conchillo.

4 recordsLinked to original sources

PubMed1995

Cytoprotective and antisecretory activity of a ranitidine-zinc complex.

The effects of a ranitidine-zinc complex and ranitidine alone were compared in three different experimental models (pyloric ligation, ethanol and indomethacin) of gastric ulceration in the rat. In the pyloric ligation model, the ranitidine-zinc complex (50, 100 and 150 mg/kg p.o.) showed antiulcerogenic activity similar to that observed with equimolar doses of ranitidine (35, 70 and 105 mg/kg p.o.). Both the ranitidine-zinc complex and ranitidine significantly reduced (p < 0.05) gastric acid secretion in a dose-dependent manner. The protective effect of the ranitidine-zinc complex (100 and 150 mg/kg p.o.) against gastric damage developing after p.o. administration of absolute ethanol or indomethacin was enhanced (p < 0.05) with respect to that obtained with equimolar doses of ranitidine (70 and 105 mg/kg p.o.). The presence of zinc in the ranitidine-zinc complex does not interfere with the antisecretory effects of ranitidine on the gastric mucosa, while it confers an additional cytoprotective action to the final compound.

Animals↗

PubMed1988

Application of thermospray/high-performance liquid chromatography/mass spectrometry to the identification of glutathione conjugates derived from bioactive epoxides.

Thermospray high-performance liquid chromatography/mass spectrometry (HPLC/MS) in the positive ion mode has proven to be a useful technique for analysing different synthetic models of potential metabolites of 3,4-epoxyprecocene II (1), the postulated bioactive epoxide responsible for the cytotoxic activity exhibited by precocenes on a variety of insect and mammal tissues. Thus, whereas standards of the glutathione adducts 3a afforded poor responses, the corresponding cysteine (3b) and N-acetylcysteine (3c) derivatives gave defined spectral patterns and good sensitivity levels, particularly when analysed as methyl esters. Likewise, 3,4-dihydrodiols (2) also afforded satisfactory responses. In all cases, the peak at m/z 237, assigned to the stabilized electrophilic oxybenzopyranyl species which results from the loss of the sulphur or oxygen substituent at C(4), was present. Finally, the incubation of racemic epoxide (1) with rat liver cytosolic fraction, followed by enzymatic degradation and further thermospray HPLC/MS analysis of the resulting methylated cysteine derivatives, allowed the identification, for the first time in a biological matrix, of the pairs of cis and trans glutathione conjugates (3a).

Acetylcysteine↗

PubMedDate not supplied

[Cholesterol oxidation products in fresh and frozen shrimps, raw and grilled].

Cholesterol oxidation products (COPs) have been related to different toxic effects, being the atherosclerotic process one of the best known. The presence of cholesterol oxides in freshly and frozenly commercialised shrimps, both raw and grilled, was studied. The determination was made by gas chromatography-mass spectrometry (GCMS). Fresh shrimps showed significant amounts of all analysed COPs, except for 7alpha-hydroxycholesterol, accounting in total for 33.15 microg COPs/g fat. In contrast, in frozen commercialised shrimps only 7-ketocholesterol and 7beta-hydroxycholesterol were detected. These results point out the great effectiveness of the commercialisation of this type of products under freezing, in terms of to the minimisation of the COPs formation. The cooking method (grilling) increased the COPs content in both types of shrimps, reaching 55.43 microg COPs/g fat in fresh shrimps and only 13.06 microg COPs/g fat in frozen ones.

Animals↗

PubMedDate not supplied

[Functional components in fish and algae oils].

An important area of the development of new functional foods is facussed on finding or applying food components which favour achieving a healthier lipid profile in the organism. The objective of this work was to carry out the characterisation of the lipid fraction of two oils, fish oil and algae oil, to evaluate their potential use as functional ingredients, in relation to the high molecular weight fatty acid content and the presence of sterols and other components of the unsaponificable fraction. Both oils showed a lipid fraction rich in high molecular weight polyunsaturated omega-3 fatty acids, containing a 33.75% in the fish oil and a 43.97% in the algae oil. Eicosapentaenoic acid was the major fatty acid in fish oil, whereas docosahexaenoic was the most abundant fatty acid in algae oil. The omega-6/omega-3 ratio was lower than 0.4 in both oils. In the unsaponificable fraction, algae oil had a Mold lower cholesterol content and a higher proportion of squalene than fish oil. The phytosterol content was significantly higher in the algae oil.

Eukaryota↗