Biomedical subjects

A Corzo

Publications and source records attributed to A Corzo.

16 recordsLinked to original sources

PubMed2006

Protein expression of pectoralis major muscle in chickens in response to dietary methionine status.

The present study evaluated the effect of dietary methionine on breast-meat accretion and protein expression in skeletal muscle of broiler chickens in vivo. All broilers received a common pre-test diet up to 21 d of age, and were subsequently fed either a methionine-deficient (MD) or -adequate (MA) diet (3.1 v. 4.5 g/kg diet) from age 21 to 42 d. Dietary cystine levels were 3.7 v. 3.6 g/kg diet for the MD and MA diet, respectively. Detrimental effects on carcass yield (P=0.004), abdominal fat percentage (P=0.001), and breast-meat weight (P=0.001), yield (P=0.001), and uniformity (P=0.002) were observed and validated in birds fed MD diets. Via tandem MS, a total of 190 individual proteins were identified from pectoralis major (PM) muscle tissue. From the former composite, peptides from three proteins were observed to be present exclusively in breast muscle from those chickens fed the MD diet (pyruvate kinase, myosin alkali light chain-1, ribosomal-protein-L-29). No proteins were observed to be uniquely expressed in chickens fed MA diets. Research is warranted to further explore the possibility of the proteins pyruate kinase, myosin alkali light chain-1, or ribosomal protein L-29, as potential biological indicators of differences in protein expression of PM of chickens in response to a dietary methionine deficiency.

Animal Nutritional Physiological Phenomena↗

PubMed2006

Valine requirements for immune and growth responses in broilers from 3 to 6 weeks of age.

Four experiments were conducted to evaluate valine (Val) requirements in Ross 508 broilers from 3 to 6 weeks of age. Common diets were fed to broilers until 3 weeks of age. Growth and carcass measurements were taken in all experiments. Immune responsiveness measurements were taken in Experiments 1 and 2. Birds given 7.2 g Val/kg of diet in Experiment 1 had more abdominal fat than birds given 8.2 g Val/kg of diet, but there were no differences in growth or other carcass measurements. Because growth performance was not reduced in birds given 7.2 g Val/kg of diet, Val concentration was reduced to 6.4 g Val/kg of diet in Experiments 2 and 3. Increasing Val from 6.4 to 8.7 g Val/kg of diet resulted in linear increases for BW gain, feed efficiency and Val intake in male birds, and Val intake in female birds. Quadratic responses to increasing dietary Val were not observed in any experiment. There were no effects of Val on innate or adaptive immunity. A nonessential amino acid mixture containing the same nitrogen content as the L-Val additions in Experiment 4 was added and tends to support the idea that the responses to Val were specific and not due to increases in total nitrogen. Dose responses to Val resulted in male, but not female, birds given 7.3 g Val/kg of diet having improved BW gain and feed efficiency compared with birds receiving 6.4 g Val/kg of diet.

Abdominal Fat↗

PubMed2006

Dietary lysine needs of late-developing heavy broilers.

Two studies were conducted to determine the response of late-developing broiler males and females to dietary Lys from 42 to 56 d of age. Hubbard Ultra Yield males and females were placed at separated ends of a floor pen broiler house and raised on common diets. At 42 d of age, broilers were then fed 1 of 7 gradient concentrations of dietary Lys that progressively increased by 0.07% from 0.68 to 1.10% total Lys. Regression analysis was performed to estimate dietary Lys needs in the presence of quadratic responses. Female broilers did not respond to dietary Lys for any variable measured. Gradient additions of Lys improved feed conversion linearly (P < 0.01) for male broilers. Fillet weight, tender weight, and their composite increased linearly (P < 0.05) with Lys supplementation in male broilers. Quadratic responses were exhibited by carcass yield, fillet yield, and total breast meat yield, resulting in total Lys optimization dietary levels of 0.88, 0.93, and 0.93%, respectively, in male broilers. Based on results from this study, high-yield male broilers should be fed a minimum of 0.93% total dietary Lys (0.85% calculated digestible) from 42 to 56 d of age. Lack of response by female broilers suggests that less dietary Lys may be needed for adequate growth and meat yield.

Animal Nutritional Physiological Phenomena↗

PubMed2005

Breeder hen dietary L-carnitine affects progeny carcase traits.

(1) Ross 308 broiler breeder hens were given diets containing 0 or 25 mg L-carnitine/kg from 21 weeks of age. (2) Hens were inseminated with semen from Ross broiler breeder males and subsequent growth performance and carcase traits, of progeny obtained from hatches at 30, 35 and 37 weeks of age, were evaluated. (3) Progeny were hatched in a common facility and separated by gender. Experimental treatments employed for the 30-, 35- and 37-week hatches, respectively, were: hen diet and progeny gender (16 replications with two subplots); hen diet, progeny diet (0 and 50 mg L-carnitine/kg of diet) and progeny gender (16 replications with 4 subplots); and hen diet and progeny diet (high and low density; 16 replications with two subplots). (4) Females had lower growth rate and less breast meat, but greater proportions of carcase fat and breast meat than males. Growth performance measurements of progeny were not affected by hen L-carnitine, but hen L-carnitine decreased abdominal fat in progeny. Increasing diet density in the chick diets increased growth and carcase weights. Hen and progeny dietary L-carnitine interacted to increase male mortality. However, dietary hen L-carnitine decreased carcase fat and increased breast meat in progeny fed on high nutrient density diets. (5) In conclusion, L-carnitine in the diet of hens affected carcase traits of their progeny.

Animal Feed↗

PubMed2005

Dietary tryptophan effects on growth and stress responses of male broiler chicks.

1. Three experiments were conducted to determine growth of broiler chicks fed on test diets formulated to be deficient or adequate in tryptophan (Trp) using gelatin by-product as a means of generating a Trp deficiency. Growth response estimates of broiler chicks to graduations of Trp were determined by dose-response criteria and regression analyses. Experiments were conducted using broiler chicks from 1 to 20 d of age. 2. Broiler chicks fed Trp-deficient diets had poor body weight gain, feed intake and feed conversion. Recommended total Trp needs were 2.0, 2.1 and 2.2 g/kg for feed intake, body weight gain and feed conversion, respectively. 3. Blood plasma Trp exhibited a sigmoidal trend while blood plasma glucose increased in a linear manner to supplemental Trp. Physiological stress variables measured were unaffected by dietary Trp.

Animal Feed↗

PubMed2005

Dietary tryptophan need of broiler males from forty-two to fifty-six days of age.

Tryptophan requirements of broiler males from 42 to 56 d of age were studied. Ross x Ross 308 chicks were placed in an open-sided house, and provided common starter and grower diets from 0 to 42 d of age. Subsequently, a corn, soybean meal, corn gluten meal, and gelatin combination of feedstuffs provided 0.12% Trp to which 0.04% increments of L-Trp were supplemented at the expense of an isonitrogenous amount of L-Glu to 0.24%. Birds that received diets containing 0.12% Trp exhibited aberrant behavior based on the spillage of considerable amounts of feed from the trough and contamination of adjacent waterers with floor litter. There were reductions in body weight gain, feed conversion, and carcass and breast fillets weights and yields with dietary Trp at 0.12%, but these were not affected at Trp levels at or above 0.16%. Exponential regression analyses showed that body weight gain improved as Trp increased, with maximum overall performance being attained at 0.17%, whereas chilled carcass weight maximized at 0.16% dietary Trp. Nitrogen retention measured using the same experimental feeds and sample birds at 48 to 49 d of age was unaffected by dietary Trp. Plasma uric acid, albumin, total protein, and aspartate-transferase measured concurrently with nitrogen retention were not altered; however, blood glucose was reduced in broilers fed 0.12% dietary Trp. Overall results suggest that broiler males need approximately 0.17% dietary Trp between 42 and 56 d of age, which closely agrees with the NRC (1994) recommendation of 0.16% Trp estimated from modeling for this feeding period.

Animal Feed↗

PubMed2005

Assessment of dietary amino acid scarcity on growth and blood plasma proteome status of broiler chickens.

Dietary Lys needs for chicks were studied. A titration diet consisting of progressive amounts of dietary Lys from 0.95% up to 1.40% was fed to broiler chicks from 0 to 18 d of age. Optimal dietary Lys level was calculated using regression analysis. Body weight gain and feed conversion were maximized at Lys levels of 1.24% (1.10% digestible) and 1.27% (1.13% digestible) of diet, respectively. Blood samples were then collected from 2 groups: birds fed the lowest Lys level and birds fed dietary Lys nearest the determined requirement level (1.25% Lys). Plasma was analyzed for protein spectra via mass spectrometry and then classified by their functional characteristics. The number of proteins was similar between the 2 samples, but there was a tendency toward increased peptides for specific proteins in plasma from chicks fed adequate Lys levels. Furthermore, after these proteins were classified, more muscle-related proteins were found in plasma samples of birds fed Lys-adequate diets. It would appear that an individual dietary amino acid deficiency does not necessarily translate into decreasing protein synthesis proportionate to body weight, but rather significant changes may be occurring within the types of proteins undergoing anabolism. In conclusion, results herein illustrate the potential for using functional genomics in nutritionally related responses of poultry.

Amino Acids↗

PubMed2005

Broiler responsiveness (Ross x 708) to diets varying in amino acid density.

Sex-separate male and female broilers (2,592 broilers; Ross x 708) were placed in 144 floor pens (12 replications per treatment) and fed diets containing high (H) and moderate (M) amino acid density from 1 to 55 d of age. Diets were formulated using ileal digestible amino acid ratios to Lys. Six dietary treatment combinations (MMMMM, HMMMM, HHMMM, HHHMM, HHHHM, and HHHHH) were implemented in 5 diet phases (1 to 5, 6 to 14, 15 to 35, 36 to 45, and 46 to 55 d of age). Male birds were heavier (P < or = 0.05) and had lower (P < or = 0.05) feed conversion, abdominal fat, and breast yield than female birds. Birds fed H diets in the first 3 phases had optimal (P < or = 0.05) BW and feed conversion (d 35, and 45), but optimal (P < or = 0.05) feed conversion at d 55 warranted H diets in all phases. Breast meat (d 35) and carcass (d 55) relative to BW were highest (P < or = 0.05) in birds fed H diets in the first 3 phases; however, differences in 55 d breast meat yield did not occur. Results indicate that amino acid needs of Ross x 708 broilers are most critical from 1 to 35 d of age. Predicted economic margins were advantageous in birds fed H diets resulting in dollar 0.12 and dollar 0.05/bird more income over feed costs at 35 and 55 d, respectively, in comparison with birds fed M diets.

Abdomen↗

PubMed2004

Isoleucine needs of thirty- to forty-day-old female chickens: immunity.

Broilers fed diets with reduced amino acid levels may be limiting in isoleucine. Because research addressing daily Ile needs for broiler immunity is sparse, Ile responses for immunity in female broilers were evaluated in 2 experiments in broilers from 30 to 42 d of age. Cellular and humoral immunity were evaluated in diets limiting in Ile and diets varying in Ile from deficient to adequate. Pen was the experimental unit in both experiments. Treatments in experiment 1 consisted of 2 levels of Ile (0.42 vs. 0.72% total of diet) and 3 strains of broilers, Arbor Acres+, Ross 508, Ross 708 (6 treatments; 5 pens each). In experiment 1, measurements consisted of: a cutaneous basophil hypersensitivity test to phytohemagglutinin-P (PHA-P) on d 37 and 38; cell quantification of CD4+, CD8+, and BU-1+ lymphocytes at d 41 and 42; and relative immune organ weights at 42 d. No Ile x strain interaction occurred. Feeding an Ile-deficient diet to broilers suppressed the cell mediated response to PHA-P, and reduced thymus weight and the percentage of CD8+ T cells. There were no significant differences between strains. In experiment 2, gradations of Ile (0.42, 0.50, 0.58, 0.66, 0.74, and 0.82% total of diet) were fed to one strain (Ross 508) of female broilers (7 pens per diet). A control diet containing 0.70% Ile (6 pens) was compared with an Ile surfeit concentration. Measurements in experiment 2 consisted of a hypersensitivity test to PHA-P on d 35 and 36; a primary antibody response to SRBC from 35 to 42 d; cell quantification of CD8+ alpha, beta, and T cell receptor (TCR)-1 (delta/gamma) lymphocytes on d 41 and 42; and immune organ weights at 42 d. Immunity measurements in birds fed surfeit Ile in the titration diets were equal to birds fed the control diet. A linear response to increasing Ile was obtained for relative bursa, but no Ile quadratic responses were noted for other measurements in experiment 2. Although feeding broilers a diet deficient in Ile suppressed some immune criteria, it does not appear that a marginal Ile deficiency will compromise immunity in growing female broilers.

Animal Feed↗

PubMed2004

Isoleucine needs of thirty- to forty-two-day-old female chickens: growth and carcass responses.

Two experiments were conducted to evaluate Ile responses in female broilers. In experiment 1, dietary Ile was 0.42 or 0.72% total of diet. Diets were fed to 3 broiler strains: multipurpose Arbor Acres+, high-yield Ross 508, and high-yield Ross 708. In experiment 2, dietary Ile dose responses (0.42 to 0.82% total of diet in 0.08% increments) were evaluated. A corn-soybean meal control (0.70%) diet was used in experiment 2 (6 replications). No Ile x strain interactions occurred. Feeding broilers 0.42% Ile suppressed (P < 0.05) BW gain, feed intake, feed conversion, and breast and thigh yields. Arbor Acres+ strain had a higher BW gain and feed intake (P < 0.05) than the Ross 508 and 708 strains. Differences (P < 0.05) among strains were observed in breast and drumstick yields; Ross 708 broilers had increased breast and drumstick yields in comparison to birds from the Arbor Acres+ and Ross 508 strains. Birds fed surfeit Ile in the titration diets grew as well as (P < 0.05) the birds fed the control diet. Quadratic responses (P < 0.05; 95% of response) were obtained for BW gain (0.67%), feed intake (0.66%), feed conversion (0.68%), and breast meat yield (0.63%). The 30- to 42-d Ile need for female broilers is between 0.63 and 0.68% of total diet (0.59 to 0.64% digestible Ile).

Animal Feed↗

PubMed2004

Valine needs of male broilers from 42 to 56 days of age.

An experiment was conducted using Ross x Ross 308 males to estimate the proportion of dietary valine needed to optimize performance in broilers from 42 to 56 d of age. All birds received common feeds from 0 to 42 d, and then experimental diets were given to 56 d of age. A diet consisting of corn, soybean meal, and corn gluten meal (17% CP, 3.25 kcal of ME/g) having 0.60% valine served as basal feed. All other essential amino acids were above recommended levels. Successive additions of 0.07% of L-valine were isonitrogenously substituted for L-glutamic acid up to a total of 0.81%. Regression analysis (95% of response) indicated that valine at 0.72% of the diet maximized body weight gain, whereas 0.73% optimized feed conversion. Depot fat removed from the abdominal cavity after processing was unaltered, and weights of resultant chilled carcasses maximized at 0.73% valine in parallel with final live weight. The amount of fillets recovered from chilled carcasses optimized at 0.73% valine; however, the incidence of distinctive blood streaks in the meat (splash) progressively increased with valine as did the level of redness apart from streaking, based on light reflectance. Given lysine at 0.85%, a ratio of 0.86 with valine appears to be adequate. The presently determined requirement of 0.73% total valine (0.67% digestible) for broiler males from 42 to 56 d of age is slightly higher than the 0.70% recommended by the NRC.

Aging↗

PubMed2004

Threonine needs of broiler chickens with different growth rates.

The Thr needs in 3 commercial broiler strains (A, multipurpose; B, high yield; C, high yield) known to differ in terms of feed intake, growth rate, and breast yield were evaluated. Birds were randomized across 96 floor pens (12 birds/pen), received a common diet from d 1 to 20, and were fed graduations of Thr (0.52 to 0.87% total Thr in 0.07% increments) from d 21 to 42. Treatments (3 x 6 factorial) were replicated 5 or 6 times. The corn, soybean meal, and peanut meal test diet contained 0.43 and 0.96% digestible Thr and Lys, respectively. An additional group of strain C birds (6 pens) was maintained on a corn-soybean meal diet containing surfeit Thr (0.73% of diet). Birds fed the corn and soybean meal diet performed similarly (P < or = 0.05) to birds fed peanut meal diets. A feed conversion interaction (P < or = 0.05) occurred indicating that strain C was more sensitive to Thr deficiency than strains A and B. The abdominal fat interaction (P < or = 0.05) indicated that strain A had more relative abdominal fat than strains B and C. All strains differed (P < or = 0.05) in terms of BW gain (A, 78.2; B, 75.1; C, 72.9 g/d). Strain C had the lowest (P < or = 0.05) feed intake, which resulted in the lowest (P < 0.05) Thr intake, but it had the highest (P < or = 0.05) breast meat yield. Most parameters tested yielded quadratic (P < or = 0.05) models whereby Thr estimates could be predicted. Namely, BW gain and breast meat yield resulted in total Thr estimates (95% of maximum response) of 0.74 and 0.71%, respectively, which are in close agreement with the 1994 NRC (0.74%). The plasma Thr sigmoid response verified the former estimates. Analysis of strain intercepts and slopes as affected by Thr differed (P < or = 0.05) in terms of feed intake but not BW gain or breast meat yield. The 21 to 42 d Thr need across strains was estimated as 0.74% total or 0.65% digestible. Because dietary Lys was not in excess of the bird's needs, the former digestibility estimate equated to a Thr/Lys of 0.68.

Abdomen↗

PubMed2004

Dietary glycine needs of broiler chicks.

Dietary Gly might become a limiting factor in all-vegetable diets fed to broiler chicks when low CP is formulated in combination with marginal levels of dietary Thr and Ser. A study was conducted to evaluate dietary Gly needs of broiler chicks. Day-old Ross 508 male chicks were placed in 32 floor pens (15 chicks/pen). Chicks were fed a common prestarter diet from 0 to 7 d of age and then fed a diet that contained progressive amounts of dietary Gly ranging from 0.62 to 1.22% from 7 to 20 d of age. Treatment effects were observed for weight gain and feed conversion. Chicks responded in a quadratic manner to supplementation with dietary Gly. The dietary Gly level necessary to support maximum growth and feed conversion for the chick from 7 to 20 d of age was estimated to be at 0.98 (1.76% Gly + Ser) and 1.02% (1.80% Gly + Ser), respectively. Plasma Thr and Ser were unaffected by Gly supplementation, but plasma free Gly increased linearly. Dietary Gly may need to be considered as a limiting nutrient in early nutrition, especially if CP is low, and only vegetable ingredients are being used.

Animal Nutritional Physiological Phenomena↗

PubMed2003

Lysine needs of summer-reared male broilers from six to eight weeks of age.

Experimentation was conducted to estimate dietary lysine needed to optimize production of summer-reared broilers between 42 to 56 d of age. Male Ross x Ross 308 chicks were placed in floor pens of an open-sided house and provided common feeds from placement to 42 d of age. During the subsequent 42 to 56 d, birds received a corn-soybean meal basal diet (18% CP and 3,250 kcal/kg ME) established to provide limiting essential amino acids favorably balanced near requirement levels with the exception of lysine. Four 0.10% increments of L-lysine isonitrogenously displaced L-glutamic acid from the basal diet to provide analyzed values progressing from 0.85 to 1.25%. Body weight gain and mortality were not altered as dietary lysine increased; however, feed conversion linearly improved. Chilled carcass yield, amount of abdominal fat, and recovery of skinless boneless breast meats were not affected. Measurements on nitrogen balance and plasma levels of total protein, albumin, glucose, and uric acid taken at 49 d from a concurrent study using sample birds in raised-wire cages and identical feeds also failed to define a requirement. However, plasma aspartic transferase increased to a maximum approximating 1.05% lysine, whereas free lysine concentration linearly increased to the highest level. Overall data supported a lysine requirement no less than 0.95% that was greater than the previous minimum of 0.85% obtained under similar terms without heat stress. Suppression of growth from heat stress appears to reduce the absolute need for lysine; however, increased dietary concentration appears necessary to accommodate depressed feed intake and improve its effectiveness.

Aging↗

PubMed2003

Arginine need of heavy broiler males: applying the ideal protein concept.

An experiment was conducted to determine arginine need of male broilers between the ages of 42 to 56 d, in conjunction with dietary protein approaching a previously advocated ideal amino acid pattern. Ross x Ross 308 chicks were reared in floor pens (32 pens with 35 birds each) of an open-sided house on common feeds until 42 d of age. From 42 to 56 d of age, birds were fed a corn-soybean meal diet (17% CP, 3,250 kcal/kg ME, and 0.85% lysine) having basal arginine at 0.80%, and then progressive additions of 0.15% were made until 1.25% was reached to form the dietary treatments. Final body weight together with body weight gain and feed conversion through the 42-to-56-d experimental period were optimized at 0.98% arginine. Weight of the chilled carcass was optimized at 1.00% arginine, whereas depot fat that had been removed from the abdominal cavity continued to decrease to the highest level of supplementation. Additional total arginine to 1.05% was needed to maximize weight recovery of fillets and total breast meat. An arginine requirement for nutritional purposes approximating 1.00% as advocated by NRC (1994) is in general agreement with present results for live production and meat yield; however, carcass incidence of skin scratch infections and parts defects from processing stresses continually responded until the highest level to suggest that additional amounts would be needed for immunological and connective tissue challenges.

Aging↗

PubMed2002

Lysine need of heavy broiler males applying the ideal protein concept.

An experiment was conducted to measure the response of broiler males to dietary lysine progressing from 0.75 to 1.15% between 42 and 56 d of age. Chicks (Ross x Ross 308) were placed in floor pens (30 pens having 35 chicks each) of an open-sided house and provided common feeds to 42 d of age. From 42 to 56 d, a corn-soybean meal diet (18% CP and 3,250 kcal/kg ME) having total lysine at 0.75% was supplemented with additions of 0.10% until 1.15%. All other essential amino adds were "ideally" balanced to one another within the limits of practicality assuming 0.85% total lysine. Birds had continuous access to feed, water, and light. Live performance during experimentation was particularly favorable. Weight gain between 42 and 56 d of age was similar among birds receiving all levels of lysine, while feed conversion was optimized at 0.85%. Depot fat removed from the abdominal cavity, yield of the resultant chilled carcass, and the amount of fillet (pectoralis major) cone deboned from the breast were unaltered by dietary lysine level. However, yield of tenders (pectoralis minor) decreased as supplemental lysine increased, whereas the incidence of myopathy (green muscle disease) increased. The lysine requirement of 0.85% as advocated by NRC (1994) for broilers between 42 and 56 d of age is in agreement with present results and may have been predisposed by its favorability of balance with all other essential amino adds.

Adipose Tissue↗