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Biomedical subjects

A Cushieri

Publications and source records attributed to A Cushieri.

5 recordsLinked to original sources
PubMed1995

Distribution of single organ malformations in European populations. EUROCAT Working Group.

The aim of this study was to evaluate the prevalence of congenital anomalies in 19 areas from 13 European countries. A total of 291,126 births were surveyed. On average most of the anomalies were recorded in livebirths. The total prevalence of congenital anomalies was 21.8 per 10,000 births. Four groups of congenital anomalies were of particular note in the consideration of geographic variations: neural tube defects, cleft lip, limb reduction defect and Down syndrome. For neural tube defects, 24 cases per 10,000 were recorded in the British Isles whereas only 9.6 cases per 10,000 were registered in Continental Europe. Cleft lip had a much higher prevalence in Groningen (The Netherlands) and in Odense (Denmark) than in the other European countries under study. The prevalence of limb reduction defect was high in Bilbao (Spain), in Odense, in Strasbourg (France) and in Groningen. In the study population the prevalence of Down syndrome ranged from 5.6 to 21.3 per 10,000 livebirths.

Cleft Lip↗
PubMed1983

Total and subtotal replacement of the common bile duct: effect of transhepatic silicone tube stenting.

The effect of silicone tube stenting on the outcome of total and subtotal replacement of the bile duct with autologous vein was investigated in experiments performed in pigs. All but one of the unstented animals developed dense strictures of the extrahepatic ductal system. This complication was abolished by the use of silicone stents passed transhepatically. The vein autografts in the stented animals underwent progressive shortening with elongation of the distal common bile duct so that beyond four months only one suture line was encountered, and no recognisable venous element was detectable on histological examination. Apart from minor dilatation of the intrahepatic biliary tree encountered in some animals, all the surviving stented animals progressed well and had normal serial liver function tests.

Animals↗
PubMedDate not supplied

Semi-permeable microcapsules for cell culture: ultra-structural characterization.

Microcapsules made from alginate-poly(L-lysine)-alginate membranes have been studied as vehicles for cell culture in a number of laboratories. We have examined their permeability, robustness and ultrastructure in detail. Permeability to globular proteins could be controlled by using poly-lysine of different mean MW in their construction. However, this parameter also affected the degree to which microencapsulated living cells leaked out of the capsules during and after preparation. Poly-lysine of low MW produced a relatively permeable and robust membrane whereas a high MW produced capsules with the reverse characteristics. A MW of 22,000 appears to be optimal in forming robust capsules which are relatively impermeable to high MW species such as immunoglobulins. The structure of the semipermeable membrane was investigated by electron microscopy and found to be complex but entirely consistent with the data on protein permeability and cell leakage. Microcapsules were not disrupted by gentle treatment with trypsin or chelating agents but dissolved with the addition of heparin, sodium dodecyl sulphate or sodium hydroxide. Empty microcapsules implanted into the peritoneal cavity of rats elicited a host cellular reaction but remained intact for at least three months.

Alginates↗