Biomedical subjects
A D Ado
Publications and source records attributed to A D Ado.
[Various mechanisms of disorders of bronchial patency].
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The responsiveness of mice B-lymphocytes to neuromediators during an immune reaction.
It was shown that increases of cAMP levels before and after adrenaline (10(-6) mol/liter) and cAMP levels before and after acetylcholine (10(-6) mol/liter) occur in mouse spleen B-lymphocytes in 2-3 and 3-6 days, respectively, after immunization with ovalbumin. Using radiolabeled blockers of these neuroreceptors, it was shown that their receptors on B-lymphocytes increase 3 and 3-4 days, respectively, after immunization. The data indicate that increases of spleen B-lymphocyte reactivity to neuromediators occur during an immune reaction associated, in part, with increases in the expression of their receptors.
[A. D. Speranskiĭ and modern immunology].
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[Infectious-allergic bronchial asthma].
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[Clinico-functional and allergological characteristics of seasonal allergic rhinitis].
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[Effect of antiglobulin serum on the expression of M-cholinoreceptors of spleen lymphocytes of intact and immunized mice].
Using a labelled blocker of M-cholinoreceptors (M-CR)--3H-Quinuclidinyl benzilate--the number of the receptors on spleen lymphocytes has been determined before and after immunization of CBA and BALB/c mice with antiglobulin serum. The incubation of non-separated spleen cell suspension with antiglobulin serum decreased the number of M-CR by 14%, while the incubation of the enriched B-lymphocyte suspension decreased it by 32.5%. The immunization of animals with ovalbumin or bovine red blood cells increased the serum effect on M-CR expression in non-separated lymphocyte suspension and had practically no influence on the serum effect in B-lymphocyte suspension. Thus, the effect on immunoglobulin receptors of B lymphocytes has a pronounced influence on M-CR expression, which may be one of the mechanisms of nervous and immune systems interaction.
[Problem of classification of various forms of bronchial asthma].
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[M-cholinergic receptors of B-lymphocytes during immune response in mice].
The number of M-cholinergic receptors on spleen B-lymphocytes of CBA mice was determined using radioactive blocker 3H-Quinuclidinil benzilate. 3 and 4 days after the animals' immunization with ovalbumin the number of M-cholinergic receptors somewhat increased. Specific antigen attenuated M-cholinergic receptor expression on B-lymphocytes, most pronounced on day 4 after immunization, without affecting the receptor expression in control animals. Possible steric interaction between antigen-binding immunoglobulins and B-lymphocyte M-cholinergic receptors during immune response is suggested.
[Gas-liquid chromatography for the determination of acetylcholinesterase activity].
Method has been modified and used for quantitative gas chromatographic determination of microtraces in a large volume of test mixture under study. The method can be applied for the differentiated determination of genuine and false blood cholinesterase by final products of specific substrate hydrolysis.
[Cholinergic variant of noninfectious allergic bronchial asthma].
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[Bronchial asthma and endorphins].
The paper deals with the investigation of endorphin content in the blood of patients with asthma. The increase in alpha- and beta-endorphin concentration was shown to depend on the severity of clinical manifestations of infectious-allergic and atopic forms of bronchial asthma. This regularity was not observed for gamma-endorphin. The infectious-allergic form of asthma was characterized by drastic reduction in the content of all three endorphin types upon treatment with dexamethasone. The possible role of endorphin reactions in the pathogenesis of asthma is discussed.
[Effect of cholino- and adrenomimetic substances on B-lymphocyte proliferation in mice during a primary immune response to a protein antigen].
A study was made of the effects of the neurotransmitters acetylcholine and adrenaline on proliferation of B lymphocytes of mice immunized with ovalbumin. The neurotransmitters were noticed to be capable of changing B cell proliferation on cultivation in vitro. The effect (an increase or reduction of 3H-thymidine incorporation) depended on the time of immunization, antigen dose, and the type of a neurotransmitter. On the whole the pattern of the acetylcholine effect correlated with a natural trend of changes in B lymphocyte proliferation at different times of the immune response. Adrenaline produced a reciprocal effect.
[Regulation of the cell cycle of B-lymphocytes in mice by substances elevating the levels of intracellular cAMP and cGMP].
Spontaneous velocity sedimentation of B lymphocytes activated by intraperitoneal injection of ovalbumin into mice was used to obtain cell cycle synchronized cells, evidenced by differences in the incorporation of labeled precursors of protein and nucleic synthesis (14C-methionine and 3H-thymidine). The effects of acetylcholine and adrenaline, cAMP and cGMP on the intensity of 3H-thymidine incorporation into mouse B lymphocytes and on the amount of the cells entering mitosis were examined. It was shown that acetylcholine is capable of stimulating whereas adrenaline of inhibitin B lymphocyte entry into the stage of DNA synthesis and egress of these cells from the stage of DNA synthesis to the stage of mitosis. Adrenaline was found to have a reciprocal action. The acetylcholine effect could be mimetized by exogenous cGMP, that of adrenaline by cAMP. Stimulation of the G1/S transition was mediated by intracellular calcium ions but did not depend on exocellular calcium.
[Interrelation of immune and mediator lymphocyte receptors in mice].
A study was made of the action of a specific muscarinic antagonist 3H-quinuclidinyl benzylate on the immune rosette formation in BALB/c mice. It was shown that treatment of mouse spleen lymphocytes by 3H-QNB at a concentration of 10(-9) M-10(-14) M brought about rosette formation inhibition. The process was dose-dependent. Atropine reversed the action of 3H-QNB.
[Effect of various types of bronchial asthma therapy on the surfactant content of the lungs].
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[External respiration in patients with Neisseria and staphylococcal bronchial asthma].
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[Immunofluorescence studies of the immunoglobulin receptors of B-lymphocytes].
The surface membrane Ig receptors of B lymphocytes in patients with atopic pollen asthma were studied by the direct immunofluorescence method. Use was made of labelled sera against human IgE, and IgG. It was shown that on the average 2.1 +/- 0.33% lymphocytes had IgE receptors, 8.4 +/- 0.63% IgG receptors and 18.7 +/- 1.16% human globulin receptors. A specific nature of B lymphocytes is suggested.