A D McNamer | PubMed Health

PubMed1974

Nicotinamide Adenine Dinucleotide-dependent Proline Dehydrogenase in Chlorella.

An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compounds. The enzyme is NAD and l-proline specific and is not an oxidase; NADP is not inhibitory. The Michaelis constant for NAD is 0.08 mm and for proline is 0.73 mm.

Journal Article↗

PubMed1973

Proline Uptake and Utilization by Chlorella pyrenoidosa.

Conditions for proline uptake and utilization by Chlorella pyrenoidosa Chick are described. Proline is taken up by growing cultures during late log phase growth after depletion of glucose from the medium. However, proline uptake by stationary phase cultures requires the presence of glucose in the medium. The results are consistent with the interpretation that some carbohydrate is required for proline uptake but proline uptake is inhibited by the accumulation of intracellular carbohydrates.Metabolism of proline was by incorporation into protein and oxidation to CO(2) by conversion to glutamic acid and subsequent metabolism of the carbon by the Krebs cycle.

Journal Article↗