PubMed Health⌕ Search

Biomedical subjects

A D Morozkin

Publications and source records attributed to A D Morozkin.

30 records · Page 2Linked to original sources
PubMedDate not supplied

[Relative location of anthroyl derivatives of stearic acid in low density lipoproteins].

Effective quenching constants (K'sv) of 2-, 7- and 12-(9-anthroyloxy)stearic acid (n-AS) fluorescence in LDL were determined. Spin probes I(m, n) (n = 3, 7, 10, 14) and I- anions were used as quenchers. Quenching of 2-AS and 12-AS fluorescence by I(m, n) was the more effective, the deeper spin probe nitroxyl fragment was located (the greater n was); maximal K'sv value corresponded to I(1,14). By contrast, for 7-AS the quenching by I(12,3) was the most effective. 2-AS and 12-AS spectra maxima and fluorescence polarization were similar. We concluded that the 2-AS chromophore was located deeper in LDL phospholipid monolayer than chromophore of 7-AS (as was the case for 12-AS).

Electron Spin Resonance Spectroscopy↗
PubMedDate not supplied

[Physico-chemical properties of low density lipoproteins isolated in an equilibrium density gradient].

The tryptophanyls of total low density lipoproteins (LDL) (1.006-1.063 g/ml) from coronary heart disease (CHD) patients and subjects without CHD signs had different accessibility to fluorescence quenchers (I-and acrylamide). LDL were separated into subfractions in equilibrium density gradient. The coefficient of extinction , quantum yield and other spectral characteristics of LDL intrinsic fluorescence, rotational mobility of maleimide spin labels and fatty acid spin probe were different in LDL subfractions from healthy subjects. LDL subfractions with hydrated density 1.045-1.05 g/ml bound to B,E-receptors of cultured fibroblasts more effectively than did subfractions with density 1.01-1.03 g/ml. Structural differences of apo-B in the particles with different lipid to protein ratio are supposed.

Apolipoproteins B↗
PubMedDate not supplied

[Fractionation of low density lipoproteins in NaBr density gradient].

The method of atherogenic class lipoproteins fractionation by equilibrium zonal centrifugation in SW 41 Ti rotor (density range 1,006-1,063 g/ml) was proposed. Hydrated densities, flotation coefficients and mean diameters of LDL particles from different subfractions were estimated.

Bromides↗
PubMedDate not supplied

[Analysis of the distribution of low density lipoprotein flotation coefficients using an analytical centrifuge equipped with absorption optics].

Procedure of polydispersity determinations of low density lipoproteins (LDL) on low concentration scale (0.04%) using analytical ultracentrifuge with absorption optics was produced. No corrections for Johnston-Ogston effect and hydrostatic compressibility effect are required. Isothermal compressibility of LDL particles was estimated to be equal to 1.9 X 10(-5) Bar-1. An equation was obtained relating the flotation coefficients of LDL from different sources with solvent density and buoyant density of their particles. It was revealed that LDL particles from individual human plasma are divided into three-four subgroups having specific flotation characteristics and particular quantities of the material in these subgroups.

Absorption↗
PubMedDate not supplied

[Low density lipoprotein distribution by flotation characteristics in normal conditions and in dyslipoproteinemias].

Distribution of low density (LDL; density range of 1.006-1.063), using the LDL flotation velocity in ultracentrifugation as a criterion, was studied in 4 groups of patients with coronary atherosclerosis (31 patients was examined, 24 of which were with angiographically confirmed coronary atherosclerosis): control group, patients with ischemic heart disease-with normal level of blood plasma lipids, with increased content of triglycerides and with decreased content of LDL cholesterol. The ratio of LDL particles with high hydration density was elevated in the LDL spectrum of all the patients examined. At the same time, each group of patients examined exhibited specific alterations in the LDL distribution spectra.

Adult↗
PubMedDate not supplied

[Genetic change in the waxy trait of barley under the influence of wild type DNA. Analysis of the composition of starch and the electrophoretic spectrum of caryopsis hordein from altered plants and the retention of these changes to the fourth generation].

Injection of DNA isolated from the wild type of barley into grains of recipient mutant plants (waxy mutants) at the milk stage of maturity leads to a change in starch synthesis; type of spikes and hordein composition. In the first generation of injected plants the wild type starch synthesis was observed in some separate plants (these observations were made at a haploid level in pollen cells). In the second generation of transformed plants along with the change in starch and hordein synthesis a modification of the type of spikes was also revealed. Recipient plants had six-rowded (hexastichous) spikes, and donor plants--two rowded (distichous) spikes. Disc-electrophoresis of hordeins of the wild type barley (Yuzhny var.), hordeins of the waxy mutant (defected in synthesis of normal starch) and barley plants transformed under the action of wild type exogenous DNA reveals differences in the protein spectrum between donor, recipient and transformants. In the second generation in many of the transformed plants starch synthesis reverted to the recipient mutant type. Simultaneously a reversion of hordein composition to the initial mutant type was observed, and the distichous pikes became hexastichous. Analysis of the components of starch revealed that donor plant that have amilose and amilopectin in starch, and the recipient plants that lack amilose, can be distinguished by the spectra of light absorption of starch. For characterizing these differences the plot of absoprtions at 490 versus that at 590 nm was used. The tangens of angles of these curves for the waxy mutant were equal to 1.05 +/- 0.07 and 1.81 +/- 0.04 for the wild type barley. All transformants have a 1.78 ratio and for revertants this value was 1.02.

DNA↗