Lymphocyte subsets in lymph node hyperplasias and B cell neoplasms as determined by fluoresceinated antibodies and flow cytometry.
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Antibodies, Monoclonal↗
Biomedical subjects
A D Ponzio
Publications and source records attributed to A D Ponzio.
Detection of platelet antibodies by flow cytometric analysis.
A rapid, sensitive indirect immunofluorescence assay utilizing flow cytometry to detect immune-mediated thrombocytopenia is described. Fluorescein-conjugated F(ab')2 antihuman IgG or IgGAM is reacted with donor platelets after their incubation with test sera and the resulting immune complex is measured using flow cytometric analysis. With this technique excess of Ig on the platelet, expressed as a ratio of fluorescence of test sera/autologous control sera, was noted in 24 of 30 patients (80%) with immune thrombocytopenias caused by auto- or allo-antiplatelet antibodies. Twenty-four sera from nonimmune thrombocytopenic patients were consistently negative. This technique has the advantage of being highly reproducible and avoids the subjective interpretation inherent with manual immunofluorescence assays.
Antibodies↗
Are lymphocyte subset determinations affected by storage conditions?
The effect of overnight storage, either at room temperature or at 4 degrees C, on total T cells, helper cells, suppressor cells, and HLA-DR-positive cells was determined by flow cytometry and fluoresceinated monoclonal antibodies on peripheral whole blood samples. No differences were noted in any of the subsets regardless of storage conditions when samples were analyzed within 24 hr.
Adult↗
A quantitative immunofluorescence assay for IgM rheumatoid factor.
A quantitative immunofluorescence assay for IgM rheumatoid factor (RF) has been developed using the FIAX fluorometer. Two hundred and six (206) patient serum samples submitted for RF or antinuclear antibody testing were assayed by the fluorometric method and by the sheep cell slide agglutination test (Rheumaton, Wampole Laboratories) currently used in our clinical laboratory. There was an overall agreement of 95.1% between the 2 methods. The fluorometric RF assay is simple, sensitive, reproducible, and does not suffer from the subjectivity present in slide agglutination tests.
Agglutination Tests↗