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Biomedical subjects

A D Tamarkina

Publications and source records attributed to A D Tamarkina.

17 recordsLinked to original sources
PubMed1985

[Enzymological evaluation of the hepatotoxicity of ampicillin and its therapeutic form, roscillin, in the treatment of pyelonephritis in pregnancy].

The combined enzymological investigation including determination of the total activity of asparagine transaminase and alanine transaminase, two serum enzymes, alkaline phosphatase, gamma-glutamyl transpeptidase, acetyl cholinesterase, and butyryl cholinesterase was applied to two groups of pregnant women with pyelonephritis treated with ampicillin (12 patients) and roscillin (14 patients). The investigation was performed at the following stages: before the treatment, on the 7th and on the 12th day of the treatment. No statistically significant differences in the average values of the activity of the above enzymes at these stages were observed in patients of the both groups which indicated the absence of the hepatotoxic effect of the preparations on the patients of a group as a whole. An increase in the levels of transaminases recorded in some patients after discontinuation of the treatment course was evident of a possible cytotoxic effect of the drugs without the signs of cholestasis. The effect was connected with the initial functional renal insufficiency.

Ampicillin↗
PubMed1978

[Changes in the mole fraction ratio of lactate dehydrogenase subunits in the lymphocytes of Down's syndrome patients].

A comparative study of the total activity and mole quota ratio of lactate dehydrogenase subunits in lymphocytes of 14 patients with Down's syndrome (trisomy-21) and in 10 healthy persons is carried out. Differences in the total activity in both groups were insignificant. In patients with Down's syndrome the mole quota ratio of H and M subunits of LDH was found to be significantly altered (p greater than 0.999): H = 33.2%, M - 66,8%, as compared to 51.5% and 48.4% in the control (healthy) group respectively. These differences are evaluated as a result of changed gene expression of both loci controlling H and M polypeptide chains of heteromeric enzyme molecule.

Chemical Phenomena↗
PubMed1977

[Dosage effect of the cytoplasmic superoxide dismutase (SOD-1) gene in the erythrocytes of Down's syndrome patients].

The activity of cytoplasmic superoxydase (SOD-1) was studied in erthrocytes of 17 patients affected with Down's syndrome (trisomy 21) and in 26 healthy persons. A 1.56-fold increase of the enzyme activity was observed in the group of patients as compared with the control group. This could be explained as the dosage effect of the corresponding gene located in the chromosome 21.

Cytoplasm↗
PubMed1975

Human triploid cell strain. Phenotype on cellular level.

The complex investigation of the bilogical properties of the triploid cell strain derived from a spontaneous abortus was carried out. Cytomorphological, autoradiographic, cytochemical, biochemical and immunochemical investigation showed that, according to most of the investigated properties, triploid cells did not differ from normal diploid cells. The cells had normal form, were well orientated, revealed expressed fibrillar apparatus and viability in the culture during 15--17 passages. The decrease of the alkaline phosphatase level, increase of acid phosphatase, lactate and malatdehydrogenase and greater nuclei area were the essential differences from the control. The cells had normal mitotic cycle parameters and the antigenic spectrum was practically identical to the normal cells.

Acid Phosphatase↗
PubMed1975

[Cultured human fibroblast enzymes. III. Enzyme activity in a triploid strain].

Complex investigation of 5 enzymes was carried out in a cell strain with triploidy 69, XXY, derived from a human spontaneous abortus. The activity of 3 enzymes (acid phosphatase, lactate and malate dehydrogenases) in triploid cells proved to be significantly increased as compared to those of 3 diploid strains, whereas the activity of alkaline phosphatase was decreased. The activity of glutamate-oxalacetate transaminase did not change. The absence of the pronounced genetic dose effect and different alteration of the activities of the enzymes studied may be considered as an expression of a disbalance of enzymes in cytogenetically defective cells.

Acid Phosphatase↗
PubMed1975

[Enzymes of cultured human fibroblasts. IV. Enzyme activity in trisomy C strains].

The activity of five enzymes (AIP, AcP, GOT, LDH, MDH) was investigated in four cell strains derived from spontaneous abortuses with C-trisomy (three cell strains with trisomy 7, one--with trisomy 9). Significant differences in the activity of three enzymes were revealed. In all the strains AIP activity was lower and GOT activity--higher than in diploid strains. Lowering of AcP level was found in three strains (two cell strains with trisomy 7, one--with trisomy 9). The data obtained are evaluated as a result of disturbed regulatory interrelations in an abnormal genome.

Acid Phosphatase↗
PubMedDate not supplied

[Enzyme diagnosis of mechanical jaundice].

Combined enzymodiagnostic program--estimation of total activity of blood serum enzymes aspartate aminotransferase (AST), alanine aminotransferase (AAT), alkaline phosphatase (AP), gamma-glutamyl transferase (gamma-GT), glutamate dehydrogenase (GDH), acetyl cholinesterase (ACE), butyryl cholinesterase (BCE)--was studied to evaluate its diagnostic validity in surgical clinic. In mechanical jaundice of various origin mean values of the enzymatic activities studied as well as the enzymatic coefficients were distinctly altered as compared with control values: AST/AAT, gamma-GT/AST as well as the newer coefficients BCE/ACE, AP/gamma-GT, (formula; see text). The jaundices of tumoral and non-tumoral genesis caused markedly dissimilar alterations in the coefficients AST/AAT and (formula; see text). The data of enzymological analysis may be used for differential diagnosis in jaundices.

Adult↗