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Biomedical subjects

A D'Elia

Publications and source records attributed to A D'Elia.

At least 19 recordsLinked to original sources
PubMed2007

The use of a dedicated rectosigmoidoscope for ultrasound staging of tumours of the upper and middle third of the rectum.

OBJECTIVE: Tumours of the upper rectum, and many in the middle third, are not accessible to endorectal ultrasound staging because of the difficulty in reaching all sites of the rectum with a rigid probe. The aim of this prospective study was to assess whether using a dedicated rectosigmoidoscope, endorectal ultrasonography (ERUS) can accurately stage any rectal lesion irrespective of its distance from the anal verge. METHOD: A total of 173 consecutive patients with a primary rectal tumour were included. A rotating, high multifrequency (5.0-10 MHz) endoprobe was introduced through a dedicated rectosigmoidoscope and advanced above the lesion. A computer allowed for three-dimensional (3D) reconstruction of 2D images. Treatment was selected on the basis of 3D-ERUS findings. ERUS staging was correlated with pathological staging. RESULTS: The depth of invasion was correctly determined by 3D-ERUS in 78.2% of tumours of the lower rectum, 76.4% of tumours extending between the lower and middle third of the rectum, 80.9% of tumours of the middle third of the rectum, 78.5% of tumours extending between the middle and upper third of the rectum and 78.9% of tumours of the upper rectum. The accuracy for the absence of lymph node metastases was 81.2% for tumours of the lower rectum, 78.5% for tumours extending between the lower and middle third of the rectum, 85.7% for tumours of the middle third of the rectum, 83.3% for tumours extending between the middle and upper third of the rectum and 78.5% for tumours of the upper rectum. Analysis showed that there was no difference between the various tumour sites. CONCLUSION: Our findings indicate that using a dedicated proctosigmoidoscope, tumours of the upper and middle third of the rectum are equally accessible to ultrasonographic evaluation. The distance of the tumour from the anal verge does not influence the accuracy of examinations considered adequate by the operator.

Adult↗
PubMed2005

Vibroacoustic stimulation in normal term human pregnancy.

OBJECTIVE: To examine the effects of vibroacoustic stimulation (VAS) on fetal heart rate (FHR) in term human fetuses by computerized carditocography system. METHODS: FHR was analyzed 20 min before and 30 min after vibroacoustic stimulation using the Oxford Sonicaid System 8002 for computerized FHR measurement. Recordings were made in 31 uncomplicated pregnancies at 36-42 weeks' gestation. RESULTS: Vibroacustic stimulation of the fetus evoked a significant increase in all the parameters evaluated (number of fetal movements, of accelerations above 10 and 15 bpm, in high- and low-variability episodes, and in short-term variations). Concerning the effect of behavioural states on the response to VAS, some changes (FHR, high-variability episodes) occurred independently of behavioural states, while other parameters (accelerations >10 and 15 bpm: short-term variation) underwent statistically significant changes only for behavioural states 1F and 2F. CONCLUSIONS: Our study supports the hypothesis of a significant fetal response in normal term pregnancy, as clearly shown by computerized cardiotocography. The immediate response occurred independently of behavioural states, although some differences were present (mainly for F1 and F2 states) if the evaluation was extended in time.

Acoustic Stimulation↗
PubMed2005

Genetic thrombophilias and uterine artery Doppler velocimetry and preeclampsia.

OBJECTIVE: The aim of this study was to evaluate the correlation between genetic thrombophilic mutations, uterine artery Doppler at 24 weeks of gestation and preeclampsia. METHODS: In a case control study we performed the genetic analysis for Leiden mutation of factor V gene (FV), G20210A mutation of the prothrombin gene (PT) and C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene in 103 women that had already attended routine ultrasonography scanner at 20 weeks at our Department. RESULTS: The frequency of heterozygous carriers of the factor V Leiden was 17.4% in the women with preeclampsia and abnormal artery Doppler compared with 3.12% in the patients with normal pregnancies. This difference was statistically significant (P<0.05). The frequency of mutation G20210A of prothrombin gene was 1.5 vs. 4.3% between women with normal pregnancies and with preeclampsia. This difference is not statistically significant. The frequency of homozygous patients for the C677T mutation of MTHFR gene among the patients with preeclampsia was 21.7% and in the control group was 10.3%, but this difference is not statistically significant. No thrombophilic gene variants were found in women with preeclampsia and normal uterine artery Doppler. CONCLUSION: We demonstrated the important association between factor V Leiden mutation, abnormal uterine Doppler at 24 weeks and preeclampsia in our population.

Adult↗
PubMed2004

Serum leptin levels and uterine Doppler flow velocimetry at 20 weeks' gestation as markers for the development of pre-eclampsia.

Altered Doppler flow velocimetry of the uterine arteries during the second trimester is correlated with the risk of developing pre-eclampsia. Serum levels of leptin, a protein regulating body weight and secreted by the placenta, are higher in women with severe pre-eclampsia. We investigated whether alterations of uterine arteries' Doppler flow velocimetry during the early second-trimester scan were accompanied by changes in leptin levels, and whether these changes might be an early risk factor for pre-eclampsia. We retrospectively selected 50 women with altered uterine artery velocimetry at the second-trimester scan who subsequently developed pre-eclampsia (group A) and 100 women who did not develop pre-eclampsia, divided into two groups: 50 women with normal velocimetry at the second-trimester scan (group B) and 50 women with altered velocimetry at the second-trimester scan (group C). Serum leptin levels during the second and third trimesters and bilateral uterine artery resistance index during the second trimester were evaluated. No differences were observed in serum leptin levels in the second trimester among the three groups. During the third trimester, women in group A showed significantly higher serum leptin levels in comparison with women in groups B and C (p < 0.01). Serum leptin levels do not seem to be a useful early marker for the development of pre-eclampsia in the presence of altered uterine blood flow, and may be a late compensatory mechanism or reflect a generalized response of the trophoblast to hypoxic stimuli.

Adult↗
PubMed2004

[Screening for pre-eclampsia in a low-risk population at 24 weeks: uterine artery Doppler flow velocimetry and genetic variants of factor V, prothrombin and methylenetetrahydrofolate reductase].

AIM: Pre-eclampsia is one of the major causes of maternal and fetal morbidity and mortality. The aim of this study was to evaluate the clinical usefulness of screening of genetic thrombophilic mutations and uterine artery Doppler flow velocimetry at 24 weeks of gestation in the prediction of pre-eclampsia in low risk pregnant women. METHODS: We performed the genetic analysis for Leiden mutation of factor V gene (FV), G20210A mutation of the prothrombin gene (PT) and C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene in 103 women that had already attended routine ultrasonography scanner at 24 weeks at our Department. RESULTS: The frequency of heterozygous carriers of the Leiden FV was 17.4% in women with pre-eclampsia and abnormal artery Doppler flow velocimetry compared with 3.12% in patients with normal pregnancies. This difference was statistically significant (P<0.05). The frequency of mutation G20210A of PT gene was 1.5% vs 4.3% between women with normal pregnancies and with pre-eclampsia. This difference is not statistically significant. The frequency of homozygous patients for the C677T mutation of MTHFR gene among patients with pre-eclampsia was 21.7% and in the control group was 10.3%, but this difference is not statistically significant. No thrombophilic genes variants were found in women with pre-eclampsia and normal uterine artery Doppler flow velocimetry. CONCLUSION: We demonstrated the important association between FV Leiden mutation, abnormal uterine artery Doppler flow velocimetry at 24 weeks and pre-eclampsia in our low-risk population.

Adult↗
PubMed2001

One-step separation from lactose: recovery and purification of major cheese-whey proteins by hydroxyapatite--a flexible procedure suitable for small- and medium-scale preparations.

The recovery of cheese-whey proteins and lactose represents an important task both in environmental and in food sciences. Optimization of whey processing requires the quantitative separation of whey proteins from lactose, lower costs, harmless environmental impact, flexibility in protein recovery, and adaptability of the process to type and amount of available whey. Here we present a method based on the use of self-made, low-price, and nontoxic hydroxyapatite for one-step separation of lactose (non adsorbed) from bovine whey proteins (adsorbed). Recovery of proteins can be performed with high flexibility. Total protein fraction can be eluted with 0.4 M phosphate at pH 7.0. In alternative, proteins can be recovered in pairs with 0.4 M phosphate but at different pH's. About 56% of the proteins, primarily alpha-lactalbulmin and IgG, were eluted at pH 5.0. The other major proteins, beta-lactoglobulin and BSA, were eluted at pH 6.0. Fractions eluted with the two first eluants at pH 5.0 and pH 6.0 were applied to a Superdex 75 column for final purification by gel filtration. This method provides flexibility in whey protein recovery and quantitative separation of proteins from lactose before ultrafiltration and nanofiltration.

Animals↗
PubMed2001

Spontaneous motor activity in normal fetuses.

AIM: The aim of this work was to perform a quantitative analysis of the different activities (mouth, eye and gross body movements) in the fetuses of 15 women with normally evolving pregnancies, and also to show the existence of intrauterine neurological maturation. METHODS: At 28, 34 and 38 weeks of gestation, 15 fetuses underwent a 60-min ultrasound observation. The data from the activities observed allowed the calculation, for each patient and each type of activity, of incidence, duration and intervals; relation between these and of each fetal activity for each gestational period; and the correlation between the different findings. RESULTS: The analysis of the data showed only a significant decrease in the incidence of gross body movements and a significant increase in the incidence of mouthing movements between 28 and 38 weeks of gestation. A correlation analysis failed to show any significant correlation between the various activities at 28 weeks, but found a positive correlation between eye, gross body movements and "other mouth movements," and a negative correlation between mouthing activity and the other activities examined at 34 and 38 weeks of gestation. CONCLUSION: The authors conclude that there is a trend of fetal activities, which is an expression of fetal neurological maturation.

Adult↗
PubMed2000

Expression and function of the homeodomain-containing protein Hex in thyroid cells.

The homeodomain-containing protein Hex (also named Prh) is expressed in primitive endoderm (during the early phases of development), in some endoderm-derived tissues and in endothelial and hematopoietic precursors. Hex expression is exting-uished during terminal differentiation of endothelial and hematopoietic cells as well as in adult lung. Previous investigations have demonstrated that Hex is expressed during early thyroid gland development. No information has been reported on Hex expression in adult thyroid gland or on the function of this protein in follicular thyroid cells. These issues represent the focus of the present study. We demonstrate that Hex mRNA is present in rat and human adult thyroid gland as well as in differentiated follicular thyroid cell lines. In FRTL-5 cells TSH reduces Hex expression. In thyroid cell lines transformed by several oncogenes Hex expression is completely abolished. By using co-transfection assays we demonstrate that Hex is a repressor of the thyroglobulin promoter and that it is able to abolish the activating effects of both TTF-1 and Pax8. These data would suggest that Hex may play an important role in thyroid cell differentiation. Protein-DNA interaction experiments indicate that Hex is able to bind sites of the thyroglobulin promoter containing either the core sequence 5'-TAAT-3' or 5'-CAAG-3'. The DNA binding specificity of the Hex homeodomain, therefore, is more 'relaxed' than that observed in the majority of other homeo-domains.

Amino Acid Sequence↗
PubMed1999

Conservative treatment by angiographic uterine artery embolization of a 12 week cervical ectopic pregnancy.

A 32 year old woman, gravid 1, nulliparous, was admitted to our department at 11 weeks and 2 days of gestation after being diagnosed with cervical pregnancy. She was unsuccessfully treated with methotrexate for 5 days. On the fifth day after admission she underwent bilateral uterine artery angiographic embolization followed by vacuum evacuation and curettage of the cervical canal. A Foley catheter was also inserted in the cervical canal and left in place for 4 days. The patient was discharged in good condition on the seventh postoperative day.

Adult↗
PubMed1998

Computer-assisted analysis of fetal movements in intrauterine growth retardation (IUGR).

A quantitative analysis of various fetal activities (mouth, eye and gross body movements) was made in 10 IUGR human fetuses. The aim of the study was to see whether IUGR fetuses move differently to normal fetuses. Each real-time ultrasound recording lasted 1 h and the analysis of various activities was carried out during replay of video recordings by means of a specially designed computer program. The following aspects have been investigated: (1) incidence, duration and interval for each of the fetal activities described; (2) the relationship between incidence, duration and interval for each single activity; (3) the correlations between the different activities. The results were compared with a group of 10 fetuses from normal pregnancies. On quantitative evaluation no clear effects due to uncomplicated IUGR could be detected except for median duration of eye movements, which turned out to be longer in the IUGR group. The evaluation of correlations between the characteristics (incidence, duration and interval) of each activity showed a positive correlation between incidence and duration of mouthing movements in the IUGR group, not found in the normal group. The study of the correlation between different fetal activities has shown an inverse correlation between mouthing and other activities in the normal fetuses, not found in the IUGR group. We conclude that in mildly affected fetuses with no evidence of hypoxia, there are no quantitative differences compared to normal fetuses in terms of the motility studied. The only differences found were in relation to the performance of such activities and they could reflect a dysfunction of the central nervous system resulting from a metabolic disturbance.

Eye Movements↗
PubMed1997

[Behavioral states. An in utero study].

OBJECTIVE: This study aims to show how a single linear transducer and different state variables can be used to enable accurate identification of behavioural states. DESIGN: Prospective observation study. SETTING: Clinic of Obstetrics and Gynaecology III at the "Federico II" University of Naples. SUBJECTS: Fifteen fetuses of women hospitalized in the Clinic of Gynaecology at the "Federico II" University of Naples at the end of a full-term, uncomplicated, single pregnancy with a reliably dated last menstruation. METHODS: Ultrasound observation, registration and data processing of various fetal activities in the uterus mouth movements (sucking), other mouth movements, eye movements, gross body movements. RESULTS: The fetal activities identified correspond exactly to the criteria established for the definition of state variables. An examination of the data thus obtained shows an inverse correlation between mouth movements and the other activities, and a direct correlation between the other three variables we considered. CONCLUSIONS: The results obtained in our study show that this method can be profitably used to identify the behavioural states, in full-term, uncomplicated pregnancies.

Behavior↗
PubMed1995

Reliability of antagonistic arch impression in dental prostheses: clinical evaluation of different preimpression preparation procedures.

This study compared the influence of different methods of preimpression preparation on the quality of occlusal reproduction in irreversible hydrocolloid impressions. A total of 30 impressions of the lower dental arch of a patient were made with five different preimpression preparation procedures. Stone casts were made and analyzed. Critical comparison showed that the preimpression preparation influenced the quality of the occlusal surface of the cast. Fingerpainting the occlusal surface with fluid hydrocolloid before positioning the loaded impression tray, associated with use of a saliva ejector, reduced the incidence of macroscopic defects on the occlusal surface of the impressions.

Adult↗
PubMed1995

Considerations on the dynamics of fraud and shoplifting in adult female offenders.

This paper compares two groups of adult female offenders involved in shoplifting and fraudulent behaviour. Twenty women were studied whose first offenses occurred in their mid-life period. The major findings conclude that an unresolved mourning or loss in the context of high stress and depression is one of the commonalities in the two groups. Another commonality is that a defined classical role for these women is conflictual and the relationship established with their spouses is a repetition of their relationship with their father. The authors suggest that these types of acting out in general are a depressive equivalent, and a larger sample could duplicate the findings and collect more precise data about the nature of the relationship in the family of origin to help these women feel better in their social role.

Acting Out↗
PubMed1994

[Materials for dies in fixed prosthesis: a comparative in-vitro analysis of their detail-reproducing capacity and of their surface quality].

The authors examine the detail reproduction capacity and surface roughness of 11 commercially available materials [6 plasters (stones?), 2 epoxy resins and 3 polyurethane resins] indicated for the construction of working models with individually extractable dies. The study was performed in vitro according to the method outlined by the ADA in specification no. 19 relating to materials for elastomeric imprints. This specification describes the use of a steel test-block with which it is possible to prepare a master imprint in polyvinylsiloxane. This imprint allows the grooves of the test-block to be reproduced in the form of angular crest which represent the details to be reproduced. Both the master imprint and the samples to be tested were prepared according to the manufactures instructions. The study was performed in three stages: macroscopic analysis, microscopic analysis, profilometric analysis. The macroscopic analysis did not show any differences between the different materials tested. The microscopic analysis showed that when enlarged 40 times the resins revealed a smoother surface than the plasters. The surface quality of plasters was improved by using a hardening solution recommended by the manufacturer as an alternative to water, or by using spacer paint. The plaster materials gave excellent angular definition contrary to the epoxy and polyurethane resins. The use, where advised, of the hardening solution as an alternative to water did not alter this parameter. The electronic profilometer used for the profilometric analysis comprised a diamond sensor which, when run along the surface, recorded all roughness, translating it into chart form.(ABSTRACT TRUNCATED AT 250 WORDS)

Dental Casting Investment↗
PubMed1984

Fetal tachypnea: prognostic significance and treatment.

We report the case of a pregnant woman with class B diabetes whose fetus, examined in utero by means of real-time ultrasound, showed tachypneic breathing movements. Having considered the clinical course of this case and of those reported in literature, we put forward a hypothesis concerning the pathogenesis and the prognostic significance of fetal tachypnea.

Adult↗