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Biomedical subjects

A Daunizeau

Publications and source records attributed to A Daunizeau.

7 recordsLinked to original sources
PubMedDate not supplied

Preparative electroelution of specific protein antigens from Mycoplasma pneumoniae: use in an enzyme-linked immunosorbent assay (ELISA).

A rapid, simple and preparative method is described for the recovery of the seven highest molecular weight proteins (HMWP) from Mycoplasma pneumoniae membrane. The yield of proteins obtained was approximately 90%. The method involved the separation of M. pneumoniae proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), followed by electroelution of HMWP. These eluted antigens were used in an ELISA to measure IgG antibodies in sera from 9 blood donors and 9 patients with M. pneumoniae infection. The specificity of M. pneumoniae HMWP was examined by competition ELISA and immunoblotting with different mycoplasma species encountered in the respiratory tract.

Antibodies, Bacterial↗
PubMedDate not supplied

[Monoclonal immunoglobulin: national external quality assessment and diagnostic reasoning].

In 2003, for the scheme of the French national external quality assessment, Afssaps sent to medical laboratories a sample for which two analyses could be carried out: the electrophoresis of proteins and the characterization for monoclonal immunoglobulin. The purpose of this new approach was to make it possible to the laboratories to transpose their usual diagnostic reasoning. This survey recalled to the biologists that it is necessary to check the sensitivity of the test of electrophoresis of proteins used in first intention and to use advisedly the anti-serums anti-free chains. Moreover this operation reinforced the educational aspect of the external quality assessment, pointing out the importance to ensure the coherence of the results of these two analyses carried out with a same diagnostic aim.

Antibodies, Monoclonal↗
PubMedDate not supplied

[A further case of IgD myeloma (author's transl)].

A 76-year-old woman was hospitalized for stabilization of her diabetic condition. The results of radiological, hematological, and biochemical investigations suggested the diagnosis of an IgD myeloma. A specific immune-serum was used to demonstrate the presence of monoclonal protein of the IgD lambda type. The course of the disease was very rapid and death occurred within 19 days from acute renal failure. The authors emphasize the various factors demonstrating the original nature of this observation and conclude by insisting on the necessity to use anti-heavy chains delta and epsilon during the diagnostic investigations for minoclonal gammapathies.

Acute Kidney Injury↗