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Biomedical subjects

A De la Chapelle

Publications and source records attributed to A De la Chapelle.

At least 19 recordsLinked to original sources
PubMed1981

Radioimmunoassay for H-Y antigen.

A sensitive direct radioimmunoassay for the detection of H-Y antigen on human blood mononuclear leukocytes is described. The cells were incubated with antisera obtained by immunization of inbred female rats or mice with male cells and tissues. The bound antibodies were detected by 125I-labeled protein A. Results are expressed as cpm bound to the cells.

Antigen-Antibody Complex↗
PubMed1976

Polyploidy of the bone marrow.

In a consecutive series of 841 patients whose bone marrows were cytogenetically investigated because of verified or suspected haematological disease, 11 patients were found to have at least 10% polyploid bone marrow mitoses. The chromosome numbers varied greatly between the cells of the same patients and between the patients. In 4 cases, the number was nearly or exactly tetraploid and in 1 patient a prevalent octaploid line was seen. Structurally abnormal marker chromosomes were seen in 8 of the patients. A total of 31 bone marrow chromosome counts were performed on a young woman with acute myelomonocytic leukaemia who had had several drug-induced remissions during the 3 1/2 years of disease. The results were related to the clinical findings. On several occasions a clear-cut correlation was noted between high proportions (nearly 100%) of polyploid cells and relapse on the one hand and low proportions (as low as 0%) of polyploids and remission on the other. Of the 11 patients, 2 had chronic myeloid leukaemia, 3 acute myelomonocytic leukaemia, 3 acute myeloid leukaemia and a further 3 some other malignant haematological disorders. We conclude that polyploidy is a feature associated with rare cases of leukaemia and other malignant diseases. It is often a sign of a poor prognosis.

Adolescent↗
PubMed1975

Transplacental passage of foetal blood cells.

With the brightly fluorescent Y chromatin as a marker, the proportion of foetal lymphocytes in maternal blood was found to be 0.02-0.16% in 7 of 11 primigravidae bearing a boy, but not a single XY mitosis was found among 112,000 leucoagglutinin-stimulated mitoses from the same women. The proportion of foetal lymphocytes did not change appreciably during pregnancy (first appearance detected at 15 weeks' gestation), nor were there any unequivocal foetal erythrocytes in spite of ABO and Rh compatibility. These findings suggest that foetal lymphocytes pass actively into the mother's blood relatively early during pregnancy. The cells are protected from immunological elimination, and therefore may remain in the maternal blood for long periods. They are a valuable potential source of material for antenatal foetal diagnosis.

Adult↗
PubMed1973

Interaction of quinacrine mustard with calf thymus histone fractions.

1. Interactions of histone fractions with quinacrine mustard were investigated by fluorimetry and spectrophotometry and the results were interpreted with the aid of thinlayer chromatography. 2. Characteristic differences were found between the various histone fractions of calf thymus. The conditions that favoured histone conformational changes and aggregation, also favoured interaction between histones and the dye; low concentrations of SO(4) (2-) brought about more interaction than did Cl(-); urea, guanidinium and iodide ions were inhibitory to binding. 3. Changes in the physical state of all the quinacrine mustard-protein complexes occurred as a function of ionic strength and pH. The most salt-dependent interaction was found in the arginine-rich histone fraction. 4. The interaction of the calf thymus histone fractions with quinacrine mustard was compared with the interaction of bovine plasma albumin and protamine with quinacrine mustard. 5. The relationship between dye-binding and the aggregation of histone fractions was discussed.

Animals↗