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Biomedical subjects

A Devillechabrolle

Publications and source records attributed to A Devillechabrolle.

25 records · Page 2Linked to original sources
PubMedDate not supplied

Respective roles of immune and nutritional factors in the priming of the immune response in the elderly.

Ninety-six institutionalized elderly (greater than 70 years old) (mean age: 82 +/- 7 years) subjects, negative for tetanus toxoid antibodies were primed with tetanus toxoid vaccination and dinitrochlorobenzene (DNCB). Correlations were studied between some immunological parameters, nutritional parameters prior to immunization and the immune response intensity after it. Levels of tetanus toxoid specific IgG (ELISA assay) were positively correlated with monocyte phagocytosis, DNCB response and prealbumin levels, and negatively correlated with total IgG, monocyte immune degradation and tetanus toxoid lymphocyte stimulation. No correlation was observed with IgA, IgM, PHA stimulation. Tetanus toxoid lymphocyte stimulation correlated positively with response to DNCB, and negatively with tetanus toxoid IgG as well as total IgG. DNCB response correlated with prealbumin, tetanus toxoid IgG and tetanus toxoid lymphocyte stimulation. Therefore, it appears that malnutrition, as measured by prealbumin level, is one of the main factors contributing to the inconstant senile immunodeficiency. Monocyte antigenic degradation function unaltered with age can impair immune response while conserved or increased phagocytosis enhances immune response in the elderly. High total IgG levels were linked with low specific responses to priming antigens. High specific antibody levels also correlated negatively with cellular specific response. It is assumed that regulatory IgG antibody accumulation, likely anti-idiotypic antibodies, play an important role in senile immunological depletion.

Aged↗
PubMedDate not supplied

Prevalence of serum antibodies to herpes simplex virus types 1 and 2: application of an ELISA technique to 100 cases of anogenital herpes.

Sera of 100 patients affected by anogenital herpes were tested by an enzyme-linked immunosorbent assay (ELISA) technique using partially purified antigens obtained by extraction from the nuclei of infected Vero cells. Herpes simplex virus type 1 (HSV-1) was isolated from the anogenital lesions of 17 patients and herpes simplex virus type 2 (HSV-2) from 83 patients. The relative proportions of antibody of the IgG class to HSV-1 and HSV-2 were determined and their ratio (R) was calculated, except for 23 patients for whom the corrected optical density (COD) obtained for HSV-1 or HSV-2 antibody was less than the lower value limit of 0.025. A predominance of HSV-2 antibody (R less than 1) was found in 40 patients and was uniformly associated with isolation of HSV-2, whereas a predominance of HSV-1 antibody (R greater than 1) in 37 patients was found in the presence of infection with HSV-1 in 11 (30%) and with HSV-2 in 26 (70%) patients. This study also confirms the association of a higher frequency of recurrences with HSV-2: among the 55 patients with recurrent herpes, those from whom HSV-2 was isolated had a history of more recurrences than those infected with HSV-1 (P less than 0.05; Mann and Whitney's nonparametric test). No correlation between the number of recurrences and the level of HSV-2 antibody was found.

Antibodies, Viral↗
PubMedDate not supplied

Detection of antibodies to human herpesvirus-6 using immunofluorescence assay.

A serological study of human herpesvirus-6 (HHV-6) infection was performed by means of immunofluorescence assay on fixed HHV-6-infected cells. Comparison between indirect immunofluorescence assay (IFA) and anticomplement immunofluorescence assay (ACIF) showed that the latter test was the most appropriate for HHV-6 serology, although both methods exhibited identical sensitivity. ACIF, which was performed on HSB2 cells infected with the HBLV strain (provided by S.Z. Salahuddin), was then used to determine the titre of HHV-6 antibodies in serum by end-point dilution. The sera from 115 healthy subjects and 99 patients with presumed viral infection were tested. A similar distribution of HHV-6 titres was evidenced in both cases and the rate of individuals exhibiting a positive titre of 20 and above was about 30% of the whole population studied. No serological cross-reactivity was observed between HHV-6 and other herpesviruses, suggesting that the HHV-6 ACIF test was quite specific.

Adult↗