Biomedical subjects
A Duarte
Publications and source records attributed to A Duarte.
[Current data on immunotherapy].
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A fertile male with cystic fibrosis: molecular genetic analysis.
A family study is presented in which the father of a girl with severe cystic fibrosis (CF) was also found to have CF but was mildly affected. He was diagnosed with three positive sweat tests including one after suppression with fludrocortisone. Genetic analysis showed that he is a compound heterozygote with the delta F508 CF mutation associated with haplotype B and a second CF mutation associated with haplotype C. In this unusual, fertile CF male, the late age of diagnosis (30 years) and the mild clinical picture suggest that the compound genotype (delta F508/other CF mutation) determines a much less severe form of the disease which might have gone unnoticed in the absence of a severely affected child. The implications of these findings for genetic counselling of families with CF are discussed.
Cystic fibrosis in the Portuguese population: haplotype distribution and molecular pathology.
The aim of this study was to obtain an estimate of the frequency of the delta F508 mutation in the Portuguese population, and of the tightness of its association with specific haplotypes. Furthermore, the genotype/clinical phenotype relationship and the feasibility of prenatal diagnosis were also investigated. The analysis of 42 cystic fibrosis (CF) families revealed that (1) 52% of CF chromosomes carry the deletion of codon 508; (2) there seems to be a positive correlation between the occurrence of the delta F508 mutation and the severity of the disease; and (3) fully informative prenatal diagnosis can be offered in 76% of at-risk pregnancies by using both genomic and allele specific oligonucleotide probes.
Apex columellar cartilage graft.
The classical rhinoplasty techniques do not give the best results when used on thick skin noses. We have developed a new technique, apex columellar cartilage graft, for correcting nasal tip projection in thick skin noses.
Atelectasis caused by acute hyperosmolality.
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Creating a free muscle flap by neovascularization: an experimental investigation.
Experimental free muscle flaps were created by inducing transmural neovascularization. Free muscle flaps were developed in Sprague-Dawley rats through a three-stage microsurgical procedure. In the first stage, a muscle strip from the external oblique abdominus muscle was elevated and folded around the superficial epigastric vessels. At six days, the second stage was performed in which the muscle flap was detached from its origin and partially elevated, demonstrating that the muscle had developed an acquired terminal axial pedicle. At 12 days, the third stage was performed in which the muscle flap on its new pedicle was transplanted to a new site. Neovascular free flap survival was documented at intervals of three hours, one day, and six days post free transfer. Plastic casts of the muscle flap vascular tree were made to demonstrate neovascularization.
Microvascular thermic sleeve anastomosis: a sutureless technique.
Microvascular thermic sleeve anastomosis performed with bipolar coagulation was investigated under experimental conditions. Patency rates of 100 percent for arteries (approximately 1.0 mm in external diameter) and 78 percent for veins (approximately 0.7 mm in external diameter) were achieved. The lumen contour was studied with plastic casts done at intervals of three hours, one day, three days, seven days, 15 days, and 30 days postoperative. Histopathologic studies were carried out to determine the changes caused by intussusception and heating spots.
Importance of vessel tension in end-to-end and end-in-end anastomoses.
Arterial end-in-end and end-to-end microanastomoses were done experimentally under natural tension and without tension. Intraluminal plastic casts were made at different postoperative time intervals, and the resulting stenosis at the site of anastomosis compared by lumen area measurements. Both anastomotic techniques revealed marked stenosis at the first hour postoperative, even when there was no tension applied to the vessels. The end-in-end technique (EIE) showed a luminal area decrease with increasing longitudinal tension. The end-to-end (ETE) did not, however, show any significant difference in stenosis, either in the nontension or the normal tension groups. No occluded anastomosis was found in either the EIE or ETE technique groups. This was remarkable, because it is during the first postoperative hour that residual luminal areas were as low as 23 percent in the successful EIE anastomoses.
Donor specific blood transfusions do not improve graft survival in living related donor transplantation.
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[The RCT--the international rehabilitation and research center for torture victims. A presentation].
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[Evaluation of the severity of trachoma in the northeastern Brazil].
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Histochemical study of the endometrium in normal cycles of Colombian women.
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