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A Dujić

Publications and source records attributed to A Dujić.

At least 37 records · Page 2Linked to original sources
PubMed1990

[Kidney transplantation in high-risk patients].

Immunosuppression with Cyclosporine A in kidney transplantation, triple therapy (CyA + Imuran + corticosteroids) and plasmapheresis before and after kidney transplantation in high risk recipients (positive cytotoxic antibody, MLC at the level of non related persons), also in high risk patients (juvenile diabetes, patients over 50 years old). In 1988 we had done in our Centre, kidney transplantation in 52.8% (28: 53) in high and increased risk patients. Triple therapy with plasmapheresis before and after kidney transplantation (if the level of cytotoxic antibodies is over 15%) allows successful kidney transplantation in high risk kidney recipients. Patients with juvenile diabetes are also available kidney recipients with therapy and permanent regulation of blood sugar. The patients of the age group between 50-60 years should be considered as suitable for kidney transplantation.

Graft Survival↗
PubMed1989

Thymic response to thermal injury in mice: I. Alterations of thymocyte subsets studied by flow cytometry and immunohistochemistry.

The dynamics of thymocyte subset changes in mice subjected to sublethal thermal injury were studied in cell suspensions by flow cytometry and in situ by immunohistochemistry. Thermal injury caused acute thymic involution in the first 2 days which was the consequence of a considerable decrease in numbers of Thyl.2high+ CD4+ CD8+, cortical thymocytes. Medullary, Thyl.2low+ thymocytes were more resistant and their relative values increased. In the regenerative phase (2-14 days) the recovery of large CD4- CD8-, early thymocytes, mainly localized in the subcapsular area of the thymus, preceded the regeneration thymocytes of the cortical phenotype. Judged by the absolute numbers of medullary thymocytes it can be seen that CD4+ CD8- (T-helper/inducer cells) were more sensitive to the effect of thermal injury than CD4- CD8+ (T-suppressor/cytotoxic cells). While values of CD4+ CD8- cells were constantly and progressively lower during 2 weeks after thermal injury, absolute numbers of CD4- CD8+ cells showed cyclic changes with lower and higher values compared to controls. An increase in the numbers of CD4- CD8+ cells was found at day 6 after thermal injury.

Animals↗
PubMed1989

Immunohistochemical identification of six cytokeratin-defined subsets of the rat thymic epithelial cells.

Rat thymic epithelial cells (TEC) have been studied by a panel of monoclonal antibodies specific for single cytokeratin polypeptides or cytokeratin pairs. Using various combinations of single and double immunostainings 6 TEC subsets (CK types) were identified, each characterized by different cytokeratin expression. Subcapsular/perivascular TEC (TEC-CK type 1) share cytokeratins 7, 8, 19 with a subset of medullary TEC, while cortical TEC were reactive with anti-CK 8 and anti-CK 18 mAbs only (TEC-CK type 2). Additional 4 subsets were identified in the medulla; TEC-CK type 3 (CK 8 + 18 + 19 +), TEC-CK type 4 (CK 8 + 10 + 18 + 19 +), TEC-CK Type 5 (CK 8 + 10 + 10 +) and TEC-CK type 6 positive only with CK 8 of all above cytokeratins. This study extends the concept of TEC heterogeneity and might also be useful to further understanding of TEC origin, development and functions.

Animals↗
PubMed1988

Immunohistochemical characterization of rat thymic non-lymphoid cells. I. Epithelial and mesenchymal components defined by monoclonal antibodies.

Molecular microenvironmental heterogeneity within rat thymus was studied by a panel of 13 monoclonal antibodies (mAbs) raised to thymic epithelial (TE) cells and mesenchymal stroma. Based on their anatomical distribution patterns observed with immunohistological techniques on frozen sections and double immunostaining using anti-keratin antibodies to identify epithelium, they were subdivided into five groups: (i) pan TE cells antibodies (R-MC 2, 3 and 8); (ii) cortical TE cells antibodies (R-MC 13-17); (iii) antibodies detecting subcapsular and subtrabecular TE cells and most medullary TE cells (R-MC 18-20); (iv) antibody to Hassall's corpuscles (HC) and a small subpopulation of medullary TE cells (R-MC 22); (v) mesenchymal stroma antibody (R-MC 23). The obtained results show phenotypic heterogeneity of rat thymic epithelium and its distinction compared to mesodermal-derived compartment.

Animals↗
PubMed1987

The effect of the rat-cyclophosphamide serum on the rat hematopoietic tissue recovery, previously damaged by cyclophosphamide or by gamma-irradiation.

The effect of post-cyclophosphamide serum (post-CY serum) humoral activity in vivo, on the rat hematopoietic regeneration after damage either by cyclophosphamide (CY) or by gamma-irradiation was investigated. Three experimental groups of rats were studied. The first group was treated by a single high dose of CY (200 mg/kg), the second group was whole body irradiated by 6.5 Gy, and the third group was whole body irradiated by 3.5 Gy. Each group was subdivided into three subgroups. The first among subgroups received immediately after damage 0.5 cc post-CY serum, the second received 0.5 normal rat sera per animal. The third subgroup of rats was used as control. Post-CY sera were collected from the Wistar rats, 2.5 months old 48 hours after the treatment by high single dose by CY (300 mg/kg). Post-CY serum activity in comparison to normal serum activity was evaluated in vivo conditions. In order to take samples of peripheral blood, spleen and bone marrow, the animals were sacrificed 24 hours, 48 hours, 72 hours and seven days after treatment. The assessment of post-CY sera effect is accomplished by measuring of the proliferative capacity of splenic, as well as bone marrow cells, and by means of the RBC and WBC counts. The results obtained indicate substantial stimulative and proliferative activity of the post-CY sera, when compared to normal sera, particularly in the animals previously damaged with CY.

Animals↗
PubMed1982

A laser nephelometric method for measuring alternative pathway complement activity of human and murine serum.

A kinetic method for the evaluation of alternative pathway complement activity in man and mice is presented. A laser nephelometer was employed for detection of non-sensitized rabbit erythrocyte lysis based on the observation that the intensity of laser scatter (LS) is proportional to the number of erythrocytes in suspension. During erythrocyte lysis a continuous decline in LS is observed since lytic products do not evoke LS. Utilizing the indirect Coombs test and cross-electrophoresis it was shown that rabbit erythrocyte lysis causes activation of the alternative complement pathway. This method is modified for room temperature, is independent of sample hemoglobin content and, in its micro version, it can be done with 10 microliter of human serum, i.e. 50 microliters of murine serum.

Animals↗
PubMed1977

[Analysis the incidence of HL-A-system antigen incidence in children treated for acute lymphoblastic leukemia].

Correlation between some histocompatible antigens and leukemia has been described both in men and in experimental animals. In 1970 Walford found an exceptionally high association between the illness and antigens of the HLA system in children with acute leukemia who had long life duration. It is therefore supposed that these antigens should not be associated with predispositional factors of the illness, instead, they are more likely to have influence over the course of the illness itself. Consequently, the authors have examined 15 children of various age who had acute leukemia. All children were examined for all antigens of the first and second HLA system, as well as for ABO and Rh genotype. The patients' blood was also checked for existence of cytotoxic antigens. Frequency of certain antigens and haplotypes was found in both children and their parents so that a comparison with antigen and haplotype frequency was also made.

ABO Blood-Group System↗
PubMed1977

[HLA system antigens in Hodgkin's disease in children].

The authors investigated HLA antigens in 11 children treated of Hodgkin's disease and compared them with 540 healthy nonrelated persons. Antigens HLA-A2, HLA-A9 and HLA-A10 were found in 5 treated children (45.4%). Antigen HLA-B5 was found in 4 children (33.4%). Antigen HLA-Bw16 was found in three patients (27.2%) which is much higher percentage than that of the control group. Haplotype A10-Bw16 which had not been found in the control group was present in two patients. Four patients (36,3%) had antigen HLA-B5 in localized form (I and II clinical stage). Four children (36,3%) had antigen HLA-A10 but only in histologic type of the lymphocytic predomination. Due to the small number of the investigated patients it was not possible to draw a definite conclusion on whether there is a significant occurence of certain antigens of the HLA system in children treated of Hodgkin's disease.

Adolescent↗