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Biomedical subjects

A E Bateman

Publications and source records attributed to A E Bateman.

11 recordsLinked to original sources
PubMed1980

In vitro chemosensitivity tests on xenografted human melanomas.

An in vitro chemosensitivity test has been applied to malignant melanoma cells from 5 patients. The tumour cells were first grown as xenografts in immune-suppressed mice, so that the results of the in vitro test could be compared with precise measurements of the sensitivity of the melanoma cells when exposed to chemotherapeutic drugs in vivo in the mouse. The in vitro assay involved exposing the tumour cells to each of 8 drugs, after which cell survival was determined by colony assay in soft agar. Dose-response curves were obtained and the surviving fraction at drug levels estimated to be achieved in man was used as a measure of in vitro drug sensitivity. Significant differences among the 8 drugs were detected, and these accorded with clinical experience. The correlation of in vivo (in the mouse) and in vitro sensitivities to Melphalan and MeCCNU was also significant.

Animals↗
PubMed1979

Assays of drug sensitivity for cells from human tumours: in vitro and in vivo tests on a xenografted tumour.

A human tumour which grows as a xenograft in immune-suppressed mice and forms colonies in vitro has been used to test the correlation between 2 methods of exposure of human tumour cells to chemotherapeutic agents. In vivo exposure to drugs was achieved by injection of tumour-bearing mice with each of 8 cytotoxic agents. For the in vitro exposure, cell suspensions were incubated for 1 h with the same series of drugs. The survival of tumour clonogenic cells was assayed in vitro after either treatment or dose-response curves were obtained. The 8 drugs were ranked according to their in vivo effect at doses equitoxic to mice, and according to their in vitro effect at concentrations designed to approximate to levels of drugs in human plasma. The ranks for in vivo and in vitro exposure correlated well.

Animals↗
PubMed1978

The proliferative state of clonogenic cells in the Lewis lung tumour after treatment with cytotoxic agents.

The proportion of clonogenic cells from the Lewis lung carcinoma which are in S-phase of the cell cycle has been measured as the fraction killed by a short exposure to hydroxyurea in vitro. Estimates of the proportions of S-phase cells before and 30 min after doses of gamma-radiation of 1000--2000 rad suggest no alternation in the cell cycle age distribution due to these doses of radiation. As the survivors of these high doses of radiation are predominantly hypoxic, the results imply that hypoxic cells have the same cell cycle age distribution as oxygenated cells in Lewis lung tumours. After treatment with cyclophosphamide or CCNU, the proportion of S-phase cells among the survivors exceeds the faction of S-phase cells in untreated populations. This increase is consistent with a relative resistance of S-phase cells to alkylating agents and nitrosoureas.

Animals↗
PubMed1976

An inhibitor of DNA synthesis in erythrocyte-conditioned medium and its separation from haemoglobin.

Erythrocyte-conditioned medium and erythrocyte lysate have been prepared from rat red blood cells. Molecular fractions of the conditioned medium and the lysate are used to test for the presence of erythrocyte chalone in short-term tissue cultures of mouse foetal liver cells. An inhibitor of erythroblast DNA synthesis has been found in the erythrocyte conditioned medium. The inhibitor is not haemoglobin, and it is not found in erythrocyte lysate soluble components.

Animals↗