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Biomedical subjects

A E Dewar

Publications and source records attributed to A E Dewar.

8 recordsLinked to original sources
PubMed1979

Properties of anti-hairy cell serum.

An antiserum was prepared in rabbits against hairy leukaemic cells. After absorption with human erythrocytes, thymus and liver powder, the serum was passed through immunoabsorbent columns to remove extensive anti-plasma protein activity. Following these procedures, sensitive techniques failed to reveal residual activity against alpha-macroglobulins, beta2-microglobulin and other human plasma proteins. The antiserum reacted with normal B lymphocytes but not with T lymphocytes or monocytes. Positive reactions were seen in B cell lymphomas, chronic lymphocytic leukaemia, hairy-cell leukaemia and uninucleated Reed-Sternberg cells.

B-Lymphocytes↗
PubMed1978

Surface membrane characteristics of cells from human acute monocytic leukaemia.

The clinical and laboratory features of 4 patients with acute monocytic leukaemia are described. Surface membrane receptor studies of these patients have shown increasing strength of receptor expression occurs with increasing morphological maturity of the monocyte. In addition, the study shows that expression of membrane receptors can vary from patient to patient and also with time and treatment in an individual patient. A further finding has been the loss of positivity with specific anti-monocyte serum when the disease becomes refractory to therapy.

Adult↗
PubMed1978

Cell receptor studies on six anaplastic tumours of the thyroid.

Cell suspensions from six anaplastic thyroid tumours were studied for expression of lymphocyte and macrophage surface markers, and results were correlated with electron microscopy, clinical extent of disease, and response to radiotherapy. The clinical presentation of the disease was similar in all six patients. In five cases, many of the cells showed surface immunoglobulin. Electron microscopy was available on three of these and showed appearances in keeping with malignant lymphoma. The single case whose cells did not show surface immunoglobulin had an entirely different ultrastructure and was probably a carcinoma. This case was the only one that did not achieve complete remission with radiotherapy and the patient died from extensive local recurrence. It is concluded that receptor techniques are of value in distinguishing between malignant lymphoma and other anaplastic tumours of the thyroid, particularly when the results are correlated with histology.

Aged↗
PubMed1977

Activated T-cell in autoimmune haemolytic anaemia.

In 12 of 14 patients with clinically active autoimmune haemolytic anaemia high levels (9-78%) of homologous and autologous rosetting cells were found in the peripheral blood. In 10 of these patients, who were observed over a period of 2 years, the levels bore a direct relationship to the activity of the disease. The cells so identified were T-lymphocytes. The role of cells, identified by this method in AIHA, is discussed.

Adolescent↗
PubMed1976

The effect of cholesterol oleate treatment of mice on the rosette forming cell response against sheep erythrocytes.

Mice injected with a single dose of 60 mg cholesterol oleate emulsion showed substantial blockade of the monoclear phagocyte system measured by the rate of vascular clearance of radio-labelled sheep erythrocytes. The labelled rythrocytes, in lipid treated mice, localized mainly in the spleen, contrasting with control mice in which localization was mainly in the liver. Treatment with this lipid, 24 hr before the intravenous of two different doses of sheep erythrocytes, resulted in significant depression of the rosette forming cell response in the spleen, whereas the responses in the lymph nodes of both control and lipid treated mice were at a low level and not significantly different. Intravenously administered cholesterol oleate emulsion is known to localize mainly in the Kupffer cells and in splenic red pulp macrophages. Cultured macrophages treated with this lipid show inhibition of antigen-binding and depressed phagocytosis of heterologous erythrocytes. The lipid does not affect lymphocytes. These findings are in keeping with the hypothesis that macrophages play a direct role in the induction of an immune response against a particulate antigen.

Animals↗