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Biomedical subjects

A E Minetti

Publications and source records attributed to A E Minetti.

At least 19 recordsLinked to original sources

The optimal locomotion on gradients: walking, running or cycling?

On level ground, cycling is more economical than running, which in turn is more economical than walking in the high speed range. This paper investigates whether this ranking still holds when moving on a gradient, where the three modes are expected to be mainly facing the same burden, i.e. to counter gravity. By using data from the literature we have built a theoretical framework to predict the optimal mode as a function of the gradient. Cycling was found to be the mode of choice only below 10-15% gradient, while above it walking was the least expensive locomotion type. Seven amateur bikers were then asked to walk, run and ride on a treadmill at different gradients. The speed was set so as to maintain almost constant the metabolic demand across the different gradients. The results indicate that the "critical slope", i.e. the one above which walking is less expensive than cycling (and running), is about 13-15%. One subject was loaded during bipedal gaits with a bicycle-equivalent mass, to simulate to cross-country cycling situation. The critical slope was close to 20%, due to the higher metabolic cost of loaded walking and running. Part of the findings can be explained by the mechanically different paradigms of the three locomotion types.

Adult↗

Interplay among the changes of muscle strength, cross-sectional area and maximal explosive power: theory and facts.

A model has recently been proposed to predict the changes of mechanical power (W) during a maximal explosive effort (such as a standing high jump off both feet) following an adaptation (e.g. training/de-training). The model is based on the assumption that, all other things being equal (ceteris paribus), the predicted changes in W depend on the measured changes of muscle force (F) or cross-sectional area (CSA) only. It follows that, if the measured changes in W are not equal to those predicted by the model, factors other than a change in F (or CSA) must be responsible for this difference. The model does not allow the determination of factors specifically involved in the adaptation process but it helps in discriminating whether an adaptation has taken place at a local level (when the observed changes in F would be attributed to factors other than the observed changes in CSA, e.g. co-contractions, fibre type modifications...), or at a central level (when the observed changes in W would be attributed to other factors than the observed changes in F, e.g. co-ordination of multiple joints and muscle groups...), or in both regions. In this paper the model has been applied to data reported in the literature on disuse (BR, bed rest), de-conditioning (SF, space flight), strength training (ST) and de-training (DT). The results of these calculations have confirmed previous observations on the determinants of the adaptation process and further suggest: (1) that training for one specific motor task (e.g. ST) could affect the performance of a second task (e.g. a maximal explosive jump) but that, as soon as the trained motor task is terminated (DT), this ability is re-gained; and (2) that neuromuscular impairment in disuse (BR) is closer to de-training than to the de-conditioning brought about by weightlessness (SF).

Anatomy, Cross-Sectional↗

Plantar flexor activation capacity and H reflex in older adults: adaptations to strength training.

The purpose of this study was to investigate whether the voluntary neural drive and the excitability of the reflex arc could be modulated by training, even in old age. To this aim, the effects of a 16-wk strengthening program on plantar flexor voluntary activation (VA) and on the maximum Hoffman reflex (H(max))-to-maximum M wave (M(max)) ratio were investigated in 14 elderly men (65-80 yr). After training, isometric maximum voluntary contraction (MVC) increased by 18% (P < 0.05) and weight-lifting ability by 24% (P < 0.001). Twitch contraction time decreased by 8% (P < 0.01), but no changes in half relaxation time and in peak twitch torque were observed. The VA, assessed by twitch interpolation, increased from 95 to 98% (P < 0.05). Pretraining VA, also evaluated from the expected MVC for total twitch occlusion, was 7% higher (P < 0.01) than MVC. This discrepancy persisted after training. The interpolated twitch torque-voluntary torque relationship was fitted by a nonlinear model and was found to deviate from linearity for torque levels >65% MVC. Compared with younger men (24-35 yr), the H(max)- to M(max) ratio and nerve conduction velocity (H index) of the older group were significantly lower (42%, P < 0.05; and 29%, P < 0.001, respectively) and were not modulated by training. In conclusion, older men seem to preserve a high VA of plantar flexors. However, the impaired functionality of the reflex pathway with aging and the lack of modulation with exercise suggest that the decrease in the H(max)- to M(max) ratio and H index may be related to degenerative phenomena.

Adaptation, Physiological↗

How fins affect the economy and efficiency of human swimming.

The aim of the present study was to quantify the improvements in the economy and efficiency of surface swimming brought about by the use of fins over a range of speeds (v) that could be sustained aerobically. At comparable speeds, the energy cost (C) when swimming with fins was about 40 % lower than when swimming without them; when compared at the same metabolic power, the decrease in C allowed an increase in v of about 0.2 ms(-1). Fins only slightly decrease the amplitude of the kick (by about 10 %) but cause a large reduction (about 40 %) in the kick frequency. The decrease in kick frequency leads to a parallel decrease of the internal work rate ((int), about 75 % at comparable speeds) and of the power wasted to impart kinetic energy to the water ((k), about 40 %). These two components of total power expenditure were calculated from video analysis ((int)) and from measurements of Froude efficiency ((k)). Froude efficiency (eta(F)) was calculated by computing the speed of the bending waves moving along the body in a caudal direction (as proposed for the undulating movements of slender fish); eta(F) was found to be 0.70 when swimming with fins and 0.61 when swimming without them. No difference in the power to overcome frictional forces ((d)) was observed between the two conditions at comparable speeds. Mechanical efficiency [(tot)/(Cv), where (tot)=(k)+(int)+(d)] was found to be about 10 % larger when swimming with fins, i.e. 0.13+/-0.02 with and 0.11+/-0.02 without fins (average for all subjects at comparable speeds).

Adolescent↗

From bipedalism to bicyclism: evolution in energetics and biomechanics of historic bicycles.

We measured the metabolic cost (C) and mechanical work of riding historic bicycles at different speeds: these bicycles included the Hobby Horse (1820s), the Boneshaker (1860s), the High Wheeler (1870s), the Rover (1880s), the Safety (1890s) and a modern bicycle (1980s) as a mean of comparison. The rolling resistance and air resistance of each vehicle were assessed. The mechanical internal work (W(INT)) was measured from three-dimensional motion analysis of the Hobby Horse and modern bicycle moving on a treadmill at different speeds. The equation obtained from the modern bicycle data was applied to the other vehicles. We found the following results. (i) Apart from the Rover, which was introduced for safety reasons, every newly invented bicycle improved metabolic economy. (ii) The rolling resistance decreased with subsequent designs while the frontal area and, hence, aerodynamic drag was fairly constant (except for the High Wheeler). (iii) The saddle-assisted body weight relief (which was inaugurated by the Hobby Horse) was responsible for most of the reduction in metabolic cost compared with walking or running. Further reductions in C were due to decreases in stride/pedalling frequency and, hence, W(INT) at the same speeds. (iv) The introduction of gear ratios allowed the use of pedalling frequencies that optimize the power/contraction velocity properties of the propulsive muscles. As a consequence, net mechanical efficiency (the ratio between the total mechanical work and C) was almost constant (0.273 +/- 0.015s.d.) for all bicycle designs, despite the increase in cruising speed. In the period from 1820 to 1890, improved design of bicycles increased the metabolically equivalent speed by threefold compared with walking at an average pace of ca. + 0.5 ms(-1) per decade [corrected]. The speed gain was the result of concurrent technological advancements in wheeled, human-powered vehicles and of 'smart' adaptation of the same actuator (the muscle) to different operational conditions.

Adult↗

The transmission efficiency of backward walking at different gradients.

The specialized design of the bipedal system towards forward locomotion has been assessed by measuring the metabolic cost and the mechanical work of both forward and backward walking on a treadmill at seven gradients from 0 to +32%. With respect to forward locomotion, backward walking implies: (1) a higher metabolic cost particularly at level gradient, while at steeper inclines the difference decreases, (2) the same mechanical internal work despite an increased stride frequency, (3) higher mechanical external work within a gradient range from 0 to +15%, (4) lower "energy recovery", i.e. the ability to save mechanical energy by moving as an inverted pendulum, mainly in level walking, and (5) as a consequence of the above results, a decrease of the efficiency of locomotion particularly at the 0% gradient. The transmission efficiency of backward walking, relative to the forward progression, was found to be about 65% in level locomotion, while at higher gradients it increased to and was maintained at a value of about 93%. The poorer economy of level backward walking could also be explained by an impaired elastic contribution in the last part of the double contact phase, while the similarity of the two gaits on higher gradients is caused by disruption of the pendulum-like paradigm due to the trajectory geometry of the body's centre of mass progressively losing its downward portion.

Adult↗

Maximal instantaneous muscular power after prolonged bed rest in humans.

A reduction in lower limb cross-sectional area (CSA) occurs after bed rest (BR). This should lead to an equivalent reduction in maximal instantaneous muscular power (W(p)) if the body segments' lengths remain unchanged. W(p) was determined during maximal jumps off both feet on a force platform before and on days 2, 6, 10, 32, and 48 after a 42-day duration BR. CSA of thigh muscles was measured by magnetic resonance imaging before and on day 5 after BR. Before BR, W(p) was 3.63 +/- 0.43 kW or 48.6 +/- 3.3 W/kg. On days 2 and 6 after BR, W(p) was reduced by 23.7 +/- 6.9 and 22.7 +/- 5.4% (P < 0.01), respectively. Thigh extensors CSA (CSAEXT) was 16.7 +/- 4.7% (P < 0.01) lower than before. When normalized per CSAEXT, W(p) was reduced by only 4.8 +/- 4.5% (P < 0.05). By day 48 of recovery, W(p) had returned to baseline values. Therefore, if W(p) is appropriately normalized for CSA of the extensor muscles, the reduction in CSAEXT explains most of the decrease in W(p) decrease after BR. Other factors such as a deficit in neural activation or a decrease in fiber-specific tension may account for only 5% of the W(p) loss after BR.

Adult↗

Mechanical and metabolic profile of locomotion in adults with childhood-onset GH deficiency.

OBJECTIVE: The aim of the present study was to evaluate the energy cost and the mechanical work of locomotion in a group of adults with childhood-onset GH deficiency (GHD). SUBJECTS: Eight males with childhood-onset GHD (mean age+/-s.d.: 31.7+/-3.6 years; mean height: 145.1+/-6.7cm) and six age-, sex- and exercise-matched normal subjects were studied. DESIGN: GHD patients and healthy controls were requested to walk and run in the speed range of 2-11km h(-1). For each condition, simultaneous mechanical and metabolic measurements were taken. METHODS: Oxygen consumption, and mechanical internal and external work of locomotion were evaluated with standard open-circuit respirometry and three-dimensional motion analysis respectively. RESULTS: External work was not significantly different between GHD patients and healthy controls, while internal work was higher for patients at all speeds. In walking, the relationships between both the mechanical energy recovery and the metabolic cost with speed were shifted towards lower speeds in patients. As a consequence, the optimal speed of walking, i.e. the speed at which the cost of locomotion is minimum, was lower for GHD patients. Stride frequency was significantly higher (11.2-11.3%) for GHD patients at all speeds of walking and running. GHD patients were unable to run at speeds higher than 8km h(-1) for the time needed to reach a metabolic steady state. CONCLUSION: It appears that both the mechanics and energetics of locomotion in short-statured adults with childhood-onset GHD are not strikingly different from those of healthy controls, thus demonstrating a substantial 'normality' in this group of GHD patients at metabolically attainable speeds. The 'harmonic' body structure and the adherence to allometric transformations in these patients do not exclude the possibility of a different metabolic role of GH in normally statured adults with childhood-onset GHD and in those with acquired GHD, taking into account the well recognized heterogeneity of the adult GHD syndrome.

Adult↗

The relationship between mechanical work and energy expenditure of locomotion in horses.

Three-dimensional motion capture and metabolic assessment were performed on four standardbred horses while walking, trotting and galloping on a motorized treadmill at different speeds. The mechanical work was partitioned into the internal work (W(INT)), due to the speed changes of body segments with respect to the body centre of mass, and the external work (W(EXT)), due to the position and speed changes of the body centre of mass with respect to the environment. The estimated total mechanical work (W(TOT)=W(INT)+W(EXT)) increased with speed, while metabolic work (C) remained rather constant. As a consequence, the 'apparent efficiency' (eff(APP)=W(TOT)/C) increased from 10 % (walking) to over 100 % (galloping), setting the highest value to date for terrestrial locomotion. The contribution of elastic structures in the horse's limbs was evaluated by calculating the elastic energy stored and released during a single bounce (W(EL,BOUNCE)), which was approximately 1.23 J kg(-)(1) for trotting and up to 6 J kg(-)(1) for galloping. When taking into account the elastic energy stored by the spine bending and released as W(INT), as suggested in the literature for galloping, W(EL,BOUNCE) was reduced by 0.88 J kg(-)(1). Indirect evidence indicates that force, in addition to mechanical work, is also a determinant of the metabolic energy expenditure in horse locomotion.

Animals↗

The biomechanics of skipping gaits: a third locomotion paradigm?

Skipping, a gait children display when they are about four- to five-years-old, is revealed to be more than a behavioural peculiarity. By means of metabolic and biomechanical measurements at several speeds, the relevance of skipping is shown to extend from links between bipedal and quadrupedal locomotion (namely galloping) to understanding why it could be a gait of choice in low-gravity conditions, and to some aspects of locomotion evolution (ground reaction forces of skipping seem to originate from pushing the walking gait to unnaturally high speeds). When the time-courses of mechanical energy and the horizontal ground reaction force are considered, a different locomotion paradigm emerges, enabling us to separate, among the bouncing gaits, the trot from the gallop (quadrupeds) and running from skipping (bipeds). The simultaneous use of pendulum-like and elastic mechanisms in skipping gaits, as shown by the energy curve analysis, helps us to understand the low cost of transport of galloping quadrupeds.

Adult↗

A model equation for the prediction of mechanical internal work of terrestrial locomotion.

By refining a previously published model, a simple equation for the estimation of the mechanical internal work during locomotion is presented. The only input variables are the progression speed, the stride frequency and the duty factor, i.e. the fraction of the stride duration at which a foot is in contact with the ground. The inclusion of this last variable, easily measurable, allows to obtain a single equation for both walking and running. The model predictions have been compared with the mechanical internal work experimentally obtained on humans in several conditions: speeds (range 0.8-3.3 m s(-1)), gaits (walking and running) and gradients (+/-15%). The close match between the two indicates that the model equation can be used whenever a direct measurement of the mechanical internal work is unavailable.

Algorithms↗

Using leg muscles as shock absorbers: theoretical predictions and experimental results of drop landing performance.

The use of muscles as power dissipators is investigated in this study, both from the modellistic and the experimental points of view. Theoretical predictions of the drop landing manoeuvre for a range of initial conditions have been obtained by accounting for the mechanical characteristics of knee extensor muscles, the limb geometry and assuming maximum neural activation. Resulting dynamics have been represented in the phase plane (vertical displacement versus speed) to better classify the damping performance. Predictions of safe landing in sedentary subjects were associated to dropping from a maximum (feet) height of 1.6-2.0 m (about 11 m on the moon). Athletes can extend up to 2.6-3.0 m, while for obese males (m = 100 kg, standard stature) the limit should reduce to 0.9-1.3 m. These results have been calculated by including in the model the estimated stiffness of the 'global elastic elements' acting below the squat position. Experimental landings from a height of 0.4, 0.7, 1.1 m (sedentary males (SM) and male (AM) and female (AF) athletes from the alpine ski national team) showed dynamics similar to the model predictions. While the peak power (for a drop height of about 0.7 m) was similar in SM and AF (AM shows a +40% increase, about 33 W/kg), AF stopped the downward movement after a time interval (0.219 +/- 0.030 s) from touch-down 20% significantly shorter than SM. Landing strategy and the effect of anatomical constraints are discussed in the paper.

Adult↗

A theory of metabolic costs for bipedal gaits.

A simple model predicts the energy cost of bipedal locomotion for given speed, stride length, duty factor and shape factor. (The duty factor is the fraction of stride duration, for which a foot is on the ground, and the shape factor describes the pattern of force exerted on the ground). The parameters are varied to find the gait that minimizes metabolic energy cost, for each speed. A previous model by Alexander calculated the work that muscles have to do, but the metabolic cost (calculated in this paper) is more likely to be the principal criterion for gait selection. This model gives good predictions of human stride lengths, and of the speed at which we break into a run. It predicts lower duty factors and higher shape factors than are normally used, but the relationships between these gait parameters and speed parallel the empirical relationships.

Energy Metabolism↗

The interplay of central and peripheral factors in limiting maximal O2 consumption in man after prolonged bed rest.

1. The effects of bed rest on the cardiovascular and muscular parameters which affect maximal O2 consumption (VO2,max) were studied. The fractional limitation of VO2,max imposed by these parameters after bed rest was analysed. 2. The VO2,max, by standard procedure, and the maximal cardiac output (Qmax), by the pulse contour method, were measured during graded cyclo-ergometric exercise on seven subjects before and after a 42-day head-down tilt bed rest. Blood haemoglobin concentration ([Hb]) and arterialized blood gas analysis were determined at the highest work load. 3. Muscle fibre types, oxidative enzyme activities, and capillary and mitochondrial densities were measured on biopsy samples from the vastus lateralis muscle before and at the end of bed rest. The measure of muscle cross-sectional area (CSA) by NMR imaging at the level of biopsy site allowed computation of muscle oxidative capacity and capillary length. 4. The VO2,max was reduced after bed rest (-16.6%). The concomitant decreases in Qmax (-30.8%), essentially due to a change in stroke volume, and in [Hb] led to a huge decrease in O2 delivery (-39.7%). 5. Fibre type distribution was unaffected by bed rest. The decrease in fibre area corresponded to the significant reduction in muscle CSA (-17%). The volume density of mitochondria was reduced after bed rest (-16.6%), as were the oxidative enzyme activities (-11%). The total mitochondrial volume was reduced by 28.5%. Capillary density was unchanged. Total capillary length was 22.2% lower after bed rest, due to muscle atrophy. 6. The interaction between these muscular and cardiovascular changes led to a smaller reduction in VO2,max than in cardiovascular O2 transport. Yet the latter appears to play the greatest role in limiting VO2,max after bed rest (> 70% of overall limitation), the remaining fraction being shared between peripheral O2 diffusion and utilization.

Adult↗

Metabolic and mechanical aspects of foot landing type, forefoot and rearfoot strike, in human running.

The study was undertaken to assess the metabolic and the mechanical aspects of two different foot strike patterns in running, i.e. forefoot and rearfoot striking (FFS and RFS), and to understand whether there is some advantage for a runner to use one or the other of the two landing styles. Eight subjects performed two series of runs (FFS and RFS) on a treadmill at an average speed of 2.50, 2.78, 3.06, 3.33, 3.61, 3.89, 4.17 m s-1. Step frequency, oxygen uptake, mechanical work, and its two components, external and internal, were measured. No differences were found for step frequency, mechanical internal work per unit time and oxygen uptake, while external and total mechanical work per unit time were significantly higher, 7-12%, for FFS. The higher external work was the result of an increase of the work performed against both gravitational and inertial forces. As the energy expenditure was the same it has been speculated that a higher storage and release of energy takes place in the elastic structures of the lower leg with FFS. In a different series of experiments on six subjects contact time, time of deceleration and time of acceleration were measured by means of a video camera while running on the treadmill at 2.50, 3.33 and 4.17 m s-1, both FFS and RFS. Time of deceleration is similar for FFS and RFS, but contact time and time of acceleration are shorter, respectively 12 and 25%, for FFS.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

Optimum gradient of mountain paths.

By combining the experiment results of R. Margaria (Atti Accad. Naz. Lincei Memorie 7: 299-368, 1938), regarding the metabolic cost of gradient locomotion, together with recent insights on gait biomechanics, a prediction about the most economical gradient of mountain paths (approximately 25%) is obtained and interpreted. The pendulum-like mechanism of walking produces a waste of mechanical work against gravity within the gradient range of up to 15% (the overall efficiency is dominated by the low transmission efficiency), whereas for steeper values only the muscular efficiency is responsible for the (slight) metabolic change (per meter of vertical displacement) with respect to gradient. The speeds at the optimum gradient turned out to be approximately 0.65 m/s (+0.16 m/s vertical) and 1.50 m/s (-0.36 m/s vertical), for uphill and downhill walking, respectively, and the ascensional energy expenditure was 0.4 and 2.0 ml O2.kg body mass-1.vertical m-1 climbed or descended. When the metabolic power becomes a burden, as in high-altitude mountaineering, the optimum gradient should be reduced. A sample of real mountain path gradients, experimentally measured, mimics the obtained predictions.

Biomechanical Phenomena↗

Effects of stride frequency on mechanical power and energy expenditure of walking.

The energetics and mechanics of walking were investigated at different speeds, both at the freely chosen stride frequency (FCSF) and at imposed ones (up to +/- 40% of FCSF). Metabolic energy expenditure was minimized at FCSF for each speed. Motion analysis allowed to calculate: the mechanical internal work rate (Wint), needed to move the segments with respect to the body center of mass (bcm); the external work rate (Wext), necessary to move bcm in the environment; and the total work rate (Wtot), equal to Wint+Wext. Wtot explains the metabolic optimization only at high speeds, while Wext, differently from previously reported, displays minima which better predict FCSF at all speeds (exception made for 1.39 m.s-1). This is probably caused by an overestimation of Wint due to a more ballistic movement of the limbs at low speeds (and low frequencies). The tendency of Wext to increase at high frequencies is due to a persistent minimal vertical excursion of bcm (about 0.02 m, the "locomotory dead space"). While the match between mechanics and energetics (at FCSF and imposed frequencies) occurs to a certain extent, it could be improved by removing the methodological assumptions about the energy transfer between segments and by the possibility to account for the coactivation of antagonist muscles.

Adult↗