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Biomedical subjects

A E Stuart

Publications and source records attributed to A E Stuart.

At least 73 records · Page 4Linked to original sources
PubMed1976

The effect of cholesterol oleate treatment of mice on the rosette forming cell response against sheep erythrocytes.

Mice injected with a single dose of 60 mg cholesterol oleate emulsion showed substantial blockade of the monoclear phagocyte system measured by the rate of vascular clearance of radio-labelled sheep erythrocytes. The labelled rythrocytes, in lipid treated mice, localized mainly in the spleen, contrasting with control mice in which localization was mainly in the liver. Treatment with this lipid, 24 hr before the intravenous of two different doses of sheep erythrocytes, resulted in significant depression of the rosette forming cell response in the spleen, whereas the responses in the lymph nodes of both control and lipid treated mice were at a low level and not significantly different. Intravenously administered cholesterol oleate emulsion is known to localize mainly in the Kupffer cells and in splenic red pulp macrophages. Cultured macrophages treated with this lipid show inhibition of antigen-binding and depressed phagocytosis of heterologous erythrocytes. The lipid does not affect lymphocytes. These findings are in keeping with the hypothesis that macrophages play a direct role in the induction of an immune response against a particulate antigen.

Animals↗
PubMed1976

Receptor studies on 19 cases of non-Hodgkin malignant lymphocytic lymphoma.

The receptor profile of 19 cases of non-Hodgkin lymphocytic lymphoma has been described. In nodular lymphomas, the values commonly fall within normal limits. In 2 cases, the nodules separated from the paranodular tissue contained a proportion of sheep-erythrocyte-rosetting cells as well as fluorescent cells. The diffuse lymphomas show predominance of fluorescent cells but some contain numerous B and T cells. These represent 'co-operative' neoplasms. In 3 cases, a receptor profile characterized by high frequency of surface fluorescence and a low incidence of IgG and EAG receptors was noted; this may represent a pattern for a new subclass of malignant lymphoma. Tumour cells rich in surface fluorescence contained abundant ribosomes. Receptor silence was noted only in a single case.

Antibodies, Neoplasm↗
PubMed1975

Effects of simple lipids on macrophages in vitro.

Tricaprin increase the rate of spreading of macrophages on glass. The treated cells showed increased pinocytosis and are avidly phagocytic. Cholesterol oleate delays the onset of phagocytosis which however proceeds efficiently once it has begun. Ethyl palmitate induces abnormal movement and the formation of unusual club shaped pseudopodia; ethyl palmitate prevents the ingestion of antibody coated erythrocytes and it is postulated that this substance interferes with the syntheses of IgG receptors.

Animals↗
PubMed1975

Histological effects of lipids on the liver and spleen of mice.

Lipids administered by the intravenous route produced ultrastructural changes in the liver and spleen of mice. Treatment with tricaprin resulted in prominent and enlarged Kupffer cells possibly with increase numbers of dense bodies. Cholesterol oleate was found within Kupffer cells and splenic macrophages. The Kupffer cells were distended by lipid and obtruded on the sinusoidal lumen; similar changes were seen in the macrophages of the red pulp and marginal zone of the spleen. Cholesterol oleate delayed but did not prevent eventual phagocytosis or colloidal carbon; the spleen contained much more carbon than usual after treatment with this substance. Treatment with ethyl palmitate resulted in diminished retention of carbon in both liver and spleen; this lipid, unlike tricaprin or cholesterol oleate, was cytotoxic to both macrophages and lymphocytes.

Animals↗
PubMed1975

Cell receptor studies on seven cases of diffuse histiocytic malignant lymphoma (reticulum cell sarcoma).

Expression of B and T lymphocyte receptors has been studied in seven cases of reticulum cell sarcoma. In one case, surface receptors and tests of phagocytic function demonstrated the histiocytic origin of the neoplastic cells. In four cases, tumour cells expressed both B and T lymphocyte markers (two cases) or showed a normal pattern of expression of B and T lymphocyte markers. In the other two cases, lymphocyte receptors were not detected, and there was no evidence of phagocytic function: this class of receptor-silent tumours is of uncertain pathogenesis. The significance of these observations is discussed.

B-Lymphocytes↗
PubMed1975

Eosinophil chemotaxis of supernatants from cultured Hodgkin's lymph node cells.

Cultured lymph node cell supernatants from five out of six cases of Hodgkin's disease were relatively more chemotactic for peripheral blood eosinophils than neutrophils. In contrast, supernatants from two cases of lymphocytic lymphoma and four nodes showing reactive hyperplasia were chemotactic for neutrophils but had little eosinophil chemotactic activity. In most instances the dgree of eosinophil infiltration observed histologically in the Hodgkin's lymph node correlated with elaboration of eosinophil chemotactic activity from the cultured cells. Following gel-filtration of three of the Hodgkin's lymphnode supernatants, four peaks of eosinophil chemotactic activity were demonstrated in each case. One of these corresponded in molecular size to th previously described eosinophil chemotactic factor of anaphylaxis (ECF-A). It is suggested that the eosinophil chemotactic activity of cultured lymph node cell supernatants may be on value in the diagnosis and classification of Hodgkin's disease.

Anaphylaxis↗