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Biomedical subjects

A E Sukharev

Publications and source records attributed to A E Sukharev.

15 recordsLinked to original sources
PubMed1998

[Clinico-diagnostic role of ferritin and lactoferrin assays in benign and malignant affections of lungs and pleura].

Immunochemical tests of blood sera from 160 healthy residents of the town of Astrakhan were performed to determine standard quantities of ferritin and lactoferrin in the serum. Examinations of the saliva from 280 healthy subjects provided the frequency of occurrence and levels of excretory lactoferrin. These iron-containing proteins were also assessed in different biosubstrates (blood serum, sputum, saliva, pleural fluid, lung tissues) from 550 patients with benign and malignant lesions of the lungs and pleura. Tests for ferritin and lactoferrin proved useful in evaluation of bronchopulmonary inflammation activity, early detection of pulmonary and intrapleural supputation, differential diagnosis of chronic nonspecific pulmonary diseases and lung cancer. The excretory salivary lactoferrin provided sufficient information on the condition of adaptive mechanisms of local defense of the lungs in premorbid state in 420 workers of the gas-processing plant exposed to aggressive occupational pollutants.

Adult↗
PubMed1996

[Tumor-associated and acute-phase proteins in the diagnosis of cancerous exudative pleurisy].

The examination of 150 patients with pleural exudate of different origin (43 cases of malignant and 107 cases of benign genesis) was made to elucidate diagnostic value of immunochemical serum and pleural fluid estimation of four tumor-associated proteins: carcinoembryonic antigen (CEA), beta 2-microglobulin (beta 2-MG), placental alkaline phosphatase (PAP), ferritin; three acute-phase proteins: C-reactive protein (CRP), lactoferrin, fibrin degradation products (FDP); expression of epithelial membrane antigen (EMA) in e date cells using monoclonal antibodies ICO-25. Determination CEA, beta 2-MG in the serum and pleural fluid, antituberculous an bodies in biological fluids proved diagnostically valuable for verification of pleural exudate characteristics. The discriminant analysis provided formulas for this differential diagnosis. The method identification in pleural fluid of cells expressing EMA with the use of monoclonal antibodies ICO-25 was found to be 2 times more efficient in detection of cancer cells than the standard light microscopy.

Acute-Phase Proteins↗
PubMed1991

[Lactoferrin and fibrinogen degradation products in saliva].

The authors suggest a simple and convenient procedure for measuring acute-phase proteins, lactoferrin, fibrinogen and its degradation products, in mixed human saliva by the agar immunodiffusion test with appropriate monospecific antisera. Examinations of 1205 salivary samples have revealed that salivary fibrinogen degradation products and lactoferrin detection rates do not depend on the subject's sex but are growing with age and intensity of the aggressive admixture effects in inhaled air; they are also related to the intensity of the inflammatory and proliferative processes in the oral cavity and bronchopulmonary system, the values varying from 4-16 percent in health to 68.8-71.8 percent in disease. In monitoring salivary acute-phase proteins detection rates make up 100 percent in the patients and 0-4 percent in normal subjects. The authors come to a conclusion that measurements of salivary acute-phase proteins may be used in screening of the population to detect subjects at risk for further diagnostic examinations.

Acute-Phase Proteins↗
PubMed1991

[The clinico-diagnostic importance of studying the ferritin content of the blood serum and sputum in pulmonary pathology].

Immunochemical and radioimmunological assays were used to measure the content of ferritin in blood, sputum and pleural fluid in patients with different pathology as well as in tissues of the pathologically altered lungs from patients who had died of chronic nonspecific pulmonary diseases (CNPD) and lung carcinoma. The measurements of ferritin in blood and sputum provide additional possibilities of recognizing the process activity in CNPD and tuberculosis, in differential diagnosis between lung carcinoma and tuberculosis during comprehensive examination of patients. In the pathologically altered lungs, the greatest content of ferritin was discovered in the areas of pneumofibrosis associated with CNPD, in the stromal structures of the cancerous node.

Blood Donors↗
PubMed1990

[Lactoferrin and leukocytes in the blood of oncologic patients].

Peripheral blood lactoferrin level and leukocyte count were measured by enzyme immunoassay in the sera of 288 normal subjects and 247 patients with malignant tumors of various localization. Normal lactoferrin level has been found 1055 +/- 191.0 ng/ml. In the patients it was changed either higher or lower, irrespective of the tumor site. This has led the authors to a conclusion on the acute phase pattern of the time course of lactoferrin level in oncologic patients. Elevation of lactoferrin level anticipated changes in leukocyte count.

Female↗
PubMed1990

[Stimulation of the synthesis of C-reactive protein using pyrogenal in non-neoplastic diseases and cancer of the lungs].

Method of immunodiffusion titration of rabbit monospecific antiserum (The I. M. Mechnikov Central Research Institute of Vaccines and Sera of the USSR Ministry of Health) in agar was used to measure blood C-reactive protein (C-RP) level in patients with acute pneumonia (32), protracted pneumonia or activation of chronic non-specific lung pathology (101) and lung cancer (153) after a 3-4-week course of complex antiinflammatory treatment and a 10-14-day course of immunostimulation with pyrogenal (The N. F. Gamaleya Research Institute of Epidemiology and Microbiology). A decrease in the incidence (from 81.2% to 14.2%) and level (from 1.4 +/- 0.3 to 0.3 +/- 0.03 mg%) of C-RP was observed in cases of non-tumor pathology whereas in patients with lung cancer those values rose in step with tumor progression (from 72.2% and 0.4 +/- 0.07 mg% at stage I to 96.3% and 1.2 +/- 0.12 mg% at stage IV). Treatment with pyrogenal allowed to identify C-RP in patients with false-negative reaction in all subgroups. Two-week dynamic C-RP curves characterizing non-tumor and tumor pathology of the lung were plotted.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1988

[Clinical significance of the immunochemical study of serum and tissue proteins of the sputum in pulmonary pathology].

The content of normal serum proteins, acute phase (C-reactive protein, pregnancy-associated alpha 2-glycoprotein) and tissue proteins (ferritin, nonspecific tissue esterase) was studied in the sputum of 256 patients with different pulmonary pathology, over time using immunochemical methods. Protein elimination with the sputum was shown to depend upon the nature and gravity of bronchitis and pulmonary destruction. Analysis of the qualitative composition of the sputum proteins and their content can be used in pulmonology for differential diagnosis and assessment of a course of pulmonary diseases.

Acute-Phase Proteins↗
PubMedDate not supplied

[Serum lactoferrin level in normal conditions and in pathology].

Lactoferrin was estimated in human blood serum of 292 healthy persons and of 518 patients with various tumoral and non-tumoral diseases by means of an immunoenzymatic assay. The highest content of lactoferrin was detected under conditions of lymphogranulomatosis, malignant tumors of colon and acute pneumonia, while the protein concentration was decreased in impairments of liver tissue and in cancer of prostate.

Humans↗
PubMedDate not supplied

[The proteins of human mixed saliva in galvanism and yeast-induced stomatitis].

Mixed salivary pools of normal subjects and patients with galvanism, yeast-induced stomatitis, and diabetes mellitus were examined. The examinations have revealed elevated alpha-amylase levels in the patients with galvanism and still higher levels of this enzyme in yeast-induced stomatitis and diabetes mellitus. These diseases are also associated with a rise of lactoferrin content and with appearance of fibrinogen degradation products.

Adult↗
PubMedDate not supplied

[Study of salivary lactoferrin in prophylactic examinations].

Immunochemical testing of salivary samples of 73 patients revealed a positive correlation between increased level of lactoferrin and the presence of chronic pancreatoduodenal diseases (gastroduodenitis, gastroduodenal ulcers, polyps of the antral portion of the stomach, cholecystitis, cholangitis, cholelithiasis, hepatitis, and pancreatitis, correlation coefficient 0.9). The test is proposed as a cheap and informative method for screening of the above diseases.

Adult↗