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Biomedical subjects

A Engberg

Publications and source records attributed to A Engberg.

7 recordsLinked to original sources
PubMed1979

Actin in spindles of Haemanthus katherinae endosperm. II. Distribution of actin in chromosomal spindle fibres, determined by analysis of serial sections.

We have studied the arrangements of actin-containing filaments in 13 bundles of kinetochore microtubules in glycerinated, heavy meromyosin-treated Haemanthus endosperm cells: 7 bundles were in a cell at anaphase, and 6 were in a cell at metaphase. Actin-containing filaments were present in each of the 13 bundles of kinetochore microtubules: they were in amongst the microtubules in the bundle and seemed to be associated with the microtubules. Actin-containing filaments in each bundle seemed to terminate at the kinetochores. Actin-containing filaments associated with the kinetochore microtubules were of consistent polarity (the arrowheads pointed towards the kinetochores) whereas those associated with other microtubles and those not associated with microtubules did not have consistent polarity (some pointed towards the spindle pole, others pointed away from it). Roughly, there were as many individual stretches of actin-containing filaments identified per bundle of kinetochore microtubules as there were microtubules which terminated at the kinetochore. These data suggest that actin-containing filaments in spindles have a functional role. We used 2 glycerination procedures in our studies (one for each cell), and neither seemed to disrupt the basic microtubule arrangements: the arrangements of spindle microtubules seen after glycerination of Haemanthus endosperm were identical to those described previously by others in non-glycerinated glutaraldehyde-fixed Haemanthus endosperm. Thus we argue that spindle structure is not disrupted by the procedures, and therefore that the arrangements of actin-containing filaments are not artifacts of the glycerination procedures. The only difference between microtubules in glycerinated cells and microtubules in untreated cells is that there seem to be fewer in the glycerinated cells. The possible role of actin-containing filaments in the spindle is discussed.

Actins↗
PubMed1978

Intrarenal hemodynamics in the transplanted rat kidney.

Regional and single glomerular blood flow conditions in the transplanted rat kidney after various periods of cold ischemia were investigated with use of the microsphere method. Intravascular injection of a silicon rubber compound (Microfil) allowed identification and sampling of single glomeruli. The periods of ischemia were two hours (minor damage), 12 hr (intermediate damage), and 16 hr (severe damage). After two hours of cold ischemia, the regional and total renal blood flows were fairly normal. After 12 hr and 16 hr of cold ischemia, the total and regional blood flows were reduced five minutes after recirculation, the reduction being pronounced in the deep cortex and juxtamedullary glomeruli. In the 12-hr group, the blood flow showed complete restitution after 65 min, whereas in the 16-hr group, the blood flow in the inner cortex and juxtamedullary glomeruli remained decreased. An impairment of medullary circulation would seem to be an important component in the pathophysiology of acute renal failure in this model.

Animals↗
PubMed1978

Nephron function of the transplanted rat kidney.

Tubular function in the early phase (one to three hours) after transplantation of rat kidneys was analyzed with respect to glomerular filtration, vascular and tubular pressures, and excretory variables. Kidneys exposed to a short period of cold ischemia (two hours) functioned almost normally, except for a polyuria. After 12 and 16 hr of cold ischemia, nephron heterogeneity appeared with 1) "normal" tubules, 2) dilated tubules, and 3) collapsed tubules. In the "normal" tubules, the pressure was increased to 20 mm Hg, and the filtration was reduced in proportion to the mean net driving force. The dilated tubules had no filtration due to a more or less complete tubular obstruction, probably located in the thin loop of Henle and in the collecting ducts. The collapsed tubules had no filtration due to glomerular ischemia, which in turn might be the consequence of afferent arteriolar constriction. The total GFR was greatly reduced since only the "normal" tubules contributed to the total filtration. Concentrating ability and potassium secretion were also impaired. We interpreted this impairment as being due to medullary dysfunction, which would explain the isosthenuria and the impaired potassium transport.

Animals↗
PubMed1978

The use of dimethyl-sulfoxide (DMSO) in the treatment of interstitial cystitis.

Dimethyl-sulfoxide (DMSO) was used in 17 patients with interstitial cystitis. The diagnosis was made on the basis of clinical and laboratory findings and the characteristic picture with Hunner ulcera. The majority of the patients had responded poorly to other forms of conservative treatment. Subjective symptoms were controlled in 2/3 of the cases but repeated treatment was needed and 5 patients did not respond to the therapy. The DMSO treatment is an alternative worth to try and has in some cases a dramatic and lasting effect.

Administration, Topical↗
PubMed1977

Malignancy grading of epithelial bladder tumours. Reproducibility of grading and comparison between forceps biopsy, aspiration biopsy and exfoliative cytology.

107 bladder tumours cystoscopically suspected to be malignant were examined morphologically by forceps biopsy, aspiration biopsy and exfoliative cytology. The malignancy was graded from 0 through 4. The reproducibility of each method was established, and was found to be 80%, 65% and 90%, respectively. Exfoliative cytology underestimated the malignancy grade as compared with histopathology (59% of the malignant tumours were not jduged as malignant in the cytological grading), but showed no tendency towards overestimation and gave no falsely postiive diagnoses of malignancy. It is concluded that diagnosis of bladder tumours requires both biopsy and cytological techniques, since they proved complementary in a number of cases. Grading by aspiration biopsy is less reliable but may be of benefit in selected cases where forceps biopsy is less suitable.

Biopsy↗
PubMed1977

Repair of renal pelvic defects with a capsular flap.

In a case of recurrent renal pelvic tumour a renal capsular flap was successfully used to repair the defect after excision of the tumour. This technique is recommended in difficult clinical situations when sacrifice of renal parenchyma is the only other alternative.

Aged↗