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Biomedical subjects

A Eskild-Jensen

Publications and source records attributed to A Eskild-Jensen.

8 recordsLinked to original sources
PubMed2003

Renal function may not be restored when using decreasing differential function as the criterion for surgery in unilateral hydronephrosis.

OBJECTIVE: To evaluate the use of decreasing differential function (DF) as an indication for surgery in children with congenital hydronephrosis followed according to a flow chart with repeated renography and ultrasonography. PATIENTS AND METHODS: The hospital records of consecutive children (0-12 years old) who underwent an Anderson-Hynes pyeloplasty between 1993 and 2000 were reviewed. Follow-up was recommended according to a flow chart, with isotope renography and ultrasonography at 1, 3 and 6 months, and at 1 (2), 3 and 5 years of age. The diagnosis and follow-up were often at the referring hospital. The criteria for surgery included a decrease in renographic DF of > 10% of DF, and a DF of < 42%, breakthrough pyelonephritis despite antibiotic prophylaxis, pain or an anterior-posterior pelvic diameter of > 50 mm. RESULTS: Fifty-two children with unilateral hydronephrosis were included; eight had surgery because of decreasing DF of the hydronephrotic kidney. Seven children had a prenatal diagnosis. Only one of these eight children was managed according to the recommended procedures for follow-up. In two and possibly three children there was significant irreversible kidney damage since the initial renography before surgery. In one child the initial renography was at 15 months and the DF was < 10%. There was a functional improvement after surgery in three children. CONCLUSION: Few children undergo surgery for decreasing DF of the hydronephrotic kidney in this study. Failure to adhere to the follow-up schedule was common; this may jeopardize kidney function when using decreasing DF as a criterion for surgery. Therefore, not complying (by medical staff or the patient) with the follow-up should be considered when using expectant management for asymptomatic hydronephrosis. The serious consequences for renal function of not complying with follow-up can be avoided by surgery if the patient risks renal functional deterioration, by close cooperation with medical staff at referring hospitals, and by close follow-up at an early age with renography and ultrasonography.

Child↗
PubMed2001

Renal function outcome in unilateral hydronephrosis in newborn pigs. II. Function and volume of contralateral kidneys.

PURPOSE: We evaluated the compensatory response of contralateral kidney growth and function in pigs with unilateral hydronephrosis. MATERIALS AND METHODS: Unilateral partial ureteral obstruction causing severe hydronephrosis was induced at age 2 days in 12 piglets, while 10 underwent sham operation. At ages 4, 12 and 24 weeks single kidney function was assessed using 99mtechnetium diethylenetriaminepentaacetic acid differential uptake on renography combined with the glomerular filtration rate estimated from the plasma clearance of 99mtechnetium diethylenetriaminepentaacetic acid. Kidney size was measured in parallel by magnetic resonance imaging. RESULTS: At 4 weeks the glomerular filtration rate and volume of the contralateral kidneys did not differ from those in controls, although obstructed kidney function was significantly decreased. At 12 weeks the mean glomerular filtration rate plus or minus standard error of mean of the contralateral kidneys significantly increased to 1.60 +/- 0.11 versus 1.33 +/- 0.11 ml. per minute per kg. (p <0. 05), whereas kidney volume did not differ from that in sham operated controls. At 24 weeks the glomerular filtration rate and volume of the contralateral kidneys did not differ from those in controls. Glomerular filtration rate and volume of the contralateral kidneys did not correlate at 4 weeks but they correlated at 12 and 24 weeks (r = 0.94 and 0.89, respectively). CONCLUSIONS: Initially kidneys contralateral to obstructed kidneys with decreased function had no increased growth or function. Furthermore, function and volume of the contralateral kidneys were not associated at the early age. Thus, the results of our study imply that determining the size (growth) or function of the contralateral kidney at an early age does not predict function decrease in a partially obstructed kidney in this pig model.

Animals↗
PubMed2001

Partial unilateral ureteropelvic junction obstruction induced in 2-week-old piglets.

PURPOSE: We examined effects on kidney function and morphology of a partial ureteropelvic junction obstruction induced late in the postnatal nephrogenesis period of the pig. We compared the results to a similar study on pigs with obstruction induced early in the nephrogenesis period. MATERIALS AND METHODS: A total of 20 14-day-old pigs were included in the study. Fifteen animals were randomized to partial unilateral obstruction and 5 were sham operated. Kidney function and morphology were analyzed regarding glomerular filtration rate, renographic functional share and total kidney volume at the age of 4, 12 and 24 weeks. RESULTS: At age 4 weeks hydronephrosis was found in all obstructed kidneys included in the study. Four obstructed kidneys had renographic functional share 40% or greater and 1 deteriorated to less than 40% at 24 weeks. A total of 11 obstructed kidneys had a functional share less than 40%, 5 increased to a share greater than 40% at age 24 weeks and 3 had decreasing share from age 4 to 24 weeks. Single kidney glomerular filtration rate of the contralateral kidneys was significantly increased at all ages. Volume of the contralateral kidney was significantly increased at age 4 and 12 weeks. CONCLUSIONS: The variability and course of renographic functional share in kidneys subjected to partial obstruction at age 2 weeks was similar to findings obtained in pigs obstructed at age 2 days. In contrast to these our study showed compensatory increase in function and size of the contralateral kidney, suggesting that contralateral compensation is an ability gained late in the nephrogenesis period.

Animals↗
PubMed2000

Topical application of cod liver oil ointment accelerates wound healing: an experimental study in wounds in the ears of hairless mice.

The effect of topical cod liver oil ointment on the rate of wound epithelialisation and neovascularisation was studied using the hairless mouse ear wound model (experiment I). The effect of local application of vitamin A in increasing concentrations was tested in the same model (experiment II). Experiment I: Bilateral standardised full thickness dermal wounds were created on the ears of 42 mice divided into three groups: group I: 25% cod liver oil ointment (n = 10) applied topically to one ear and vehicle (vaseline) to the other; group II: 25% cod liver oil ointment (n = 10) and saline; and group III: vehicle (n = 22) and saline. Experiment II: Using the same model and procedures wounds were made on 12 mice randomised to vitamin A treatment in various doses (250-3000 IU/g) on one ear and vehicle (vaseline) on the other ear. Using in-vivo microscopy and digitised planimetry, wound epithelialisation and neovascularisation were measured at regular intervals until the processes were complete. Wounds treated with 25% cod liver oil ointment epithelialized significantly (p < 0.05) faster (mean (SEM) 8.9 (0.7) days) than control ears treated with vehicle alone (13.9 (1.9) days). Neovascularisation developed significantly faster (p < 0.01) in the ears treated with cod liver oil ointment (22.5 (1.3) days) compared with their vehicle control (29.1 (0.6) days). Neovascularisation was also significantly (p < 0.05) faster in the ears treated with cod liver oil ointment (23.1 (1.4) days) than in those treated with saline (26.8 (1.1) days). There was no significant difference in speed of epithelialisation between cod liver oil ointment and saline. The vitamin A dose study showed that epithelialisation and neovascularisation of the vitamin A treated wounds pass at the same rate as wounds treated with cod liver oil ointment. In conclusion, topical 25% cod liver oil ointment significantly accelerated both the epithelial and the vascular component of healing compared with saline. Vitamin A seems to have an important role in accelerating wound healing and could be the active component in cod liver oil.

Animals↗
PubMed2000

Renal functional outcome in unilateral hydronephrosis in newborn pigs.

PURPOSE: We followed the course of neonatally induced, partial unilateral ureteral obstruction in pigs and established whether early function or morphological evaluation of the hydronephrotic kidney is predictive of outcome. MATERIALS AND METHODS: In 25 piglets unilateral partial ureteropelvic obstruction was induced 2 days after birth, while 11 underwent sham operation. Only obstructed kidneys with significant dilatation at 4 weeks were included for further study. Renal function was assessed on renography using (99m)technetium-diaminetriaminepentaacetic acid differential uptake. According to the plasma clearance of (99m)technetium-diaminetriaminepentaacetic acid the glomerular filtration rate was determined at ages 4, 12 and 24 weeks. Kidney morphology was studied in parallel by magnetic resonance imaging. RESULTS: A total of 12 obstructed kidneys with grade 3 or 4 hydronephrosis fulfilled study inclusion criteria. Of 6 kidneys with a functional share of 40% or greater at age 4 weeks 2 deteriorated to less than 40% at 24 weeks, while 4 of 6 with a share of less than 40% at age 4 weeks improved to greater than 40% at 24 weeks. The single kidney glomerular filtration rate at 4 weeks did not correlate with that at 12 or 24 weeks but the rate at 12 weeks correlated with that at 24 weeks (r2 = 0.8140, p <0.001). Neither relative volume of the obstructed kidney nor length of the contralateral nonobstructed kidney correlated with functional share of the obstructed kidney at any age. CONCLUSIONS: Early evaluation of kidney function or volume, or contralateral kidney length did not predict the outcome of neonatally induced unilateral hydronephrosis in pigs.

Age Factors↗
PubMed1997

Endogenous TGF-beta 1 and TGF-beta 2 are not essential for epithelialization and neovascularization in the hairless mouse ear wound model.

BACKGROUND AND AIMS: TGF-beta stimulates neovascularization and epithelialization in healing wounds, yet relatively little is known about the mechanisms involved. Using the hairless mouse ear wound model, we studied the effects endogenous TGF-beta 1 and TGF-beta 2 have on epithelialization and neovascularization following the application of neutralizing antibodies. MATERIAL AND METHODS: Forty-three adult male hairless mice had an excisional wound made on the dorsum of each ear. Using vital microscopy, epithelialization and neovascularization were measured every third day until completion. TGF-beta 1 and TGF-beta 2 antibody, control-IgG, or phosphate buffered saline (PBS) were applied to the wounds. RESULTS AND CONCLUSIONS: Excisional wounds treated with anti-TGF-beta 1 and anti-TGF-beta 2, IgGcontrol IgG, and PBS epithelialized in 11.2 +/- 0.5 days (N = 22), 10.9 +/- 0.6 days (N = 17), and 10.6 +/- 0.6 days (N = 15), respectively and neovascularized in 27.9 +/- 0.5 days (N = 17), 27.1 +/- 0.8 days (N = 14), and 26.1 +/- 0.8 days (N = 10), respectively (mean +/- SEM). There were no significant differences in time to epithelialization and neovascularization between the three groups. Furthermore, there were no differences in the average time course of epithelialization and neovascularization between the three groups throughout the healing process. We conclude that endogenous TGF-beta 1 and TGF-beta 2 are not essential for epithelialization and neovascularization in the hairless mouse ear wound model.

Animals↗
PubMedDate not supplied

Surgically induced urologic models in swine.

The porcine urological system is similar anatomically and physiologically to that of humans. Swine have a true multirenculate, multipapillate kidney with a calyceal system like that of humans. The gross anatomic and histologic characteristics of the porcine and the human kidney are more similar than most other commonly used laboratory animals. The physiologic functions of the urinary system including urodynamic parameters are also similar to humans. Swine have been used extensively as models of urologic conditions in humans, most commonly in obstructive urologic syndromes. A large number of investigative procedures can be performed in one experiment both in acute and chronic models. Recently, a new technique of surgically induced antenatal lower urinary tract obstruction in swine has been developed.

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