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A Expert-Bezançon

Publications and source records attributed to A Expert-Bezançon.

26 records · Page 2Linked to original sources
PubMed1980

Synthesis and properties of N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine, a new reagent for RNA-RNA and RNA-protein cross-linking.

The synthesis of N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine (Gbz-Cyn2-Ac) is described. Like other glyoxal-type reagents it reacts with guanine and arginine. The kinetics and pH dependence of these reactions are studied. (Gbz-Cyn2-Ac) reacts with non-base-paired guanines at about 20 sites in 16-S rRNA. After reduction of disulfide bonds each derivatized guanine residue carries a free -- SH group which can be used to create RNA-RNA bridges or, after introducing an additional photoactivatable derivative, RNA-protein bridges.

Arginine↗
PubMed1975

[Nearest-neighbor relationships among 50S ribosomal proteins of E. coli].

Dimethyl suberimidate has been used to study the proximity relations between E. coli 50 S ribosomal proteins. The composition of two cross-linked protein complexes has been determined; one is a quaternary complex containg proteins L2, L20, L27 and L32-33, and the other is a ternary complex containing proteins L2, L13 and L20. The vicinity of these proteins in the ribosome is discussed.

Dimethyl Suberimidate↗