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A F Burton

Publications and source records attributed to A F Burton.

35 records · Page 2Linked to original sources
PubMedDate not supplied

Effect of the dietary fatty acid component on the release of 14C-taurocholate, 14C-bilirubin, alkaline phosphatase, and aspartate transaminase by isolated rat liver cells.

Rats were fed liquid diets for 7 days containing either triolein or Liposyn, which is rich in linoleic acid, as fat sources, and liver cell suspensions were prepared following collagenase perfusion. The release from isolated cells of alkaline phosphatase and aspartate transaminase during a 3-hr incubation did not differ. The uptake and release of 14C-taurocholate during a brief incubation was lower but not significantly in Liposyn-fed rats (0.1 greater than p greater than 0.05): the uptake was 9.74 +/- 1.58 vs 16.7 +/- 3.3 nmol/mg protein in triolein-fed rats; the release was 3.17 +/- 0.65 vs 5.35 +/- 1.01 nmol/mg protein in triolein-fed rats. The uptake of 14C-aminolevulinic acid was similar in both groups, but release of 14C-bilirubin during a 30-min incubation was 5,420 +/- 1010 in the Liposyn group vs 12,030 +/- 2,200 dpm/mg protein in the triolein group (p = 0.02). It is concluded that a diet high in linoleic acid decreases bilirubin release in isolated liver cells consistent with the ability of this diet to cause cholestasis in vivo.

Alkaline Phosphatase↗
PubMedDate not supplied

Increased cholesteryl ester content in liver of mice fed lipid emulsion diets high in polyunsaturated fats.

Mice were fed a liquid diet containing different fat sources for 6 days and several biochemical parameters in the liver were examined. Mice fed diets containing Nutralipid or Liposyn as 45% of total calories had 30.5 +/- 2.5 and 25.8 +/- 3.7 nmol cholesteryl esters per milligram liver protein, respectively, as compared with 13.14 +/- 2.4 for those fed regular mouse food and 13.7 +/- 2.45 for those fed an emulsion containing mostly triolein as fat source. A similar increase in liver cholesteryl esters resulting from estrogen treatment has been proposed as the basis for changes resulting in decreased bile flow in the rat. It is suggested that the high content of polyunsaturated fatty acids in nutrient emulsions might be responsible for cholestasis sometimes observed in patients receiving these preparations. This is further supported by the observation that, as in the case of estrogen treatment, the cholesteryl ester level returned to normal when mice were treated with the detergent Triton WR-1339.

Animals↗