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Biomedical subjects

A F Bykovskiĭ

Publications and source records attributed to A F Bykovskiĭ.

At least 19 recordsLinked to original sources
PubMed1991

[The effect of interferon preparations on the ultrastructural organization of Legionella pneumophila].

The influence of the preparations of interferon on morphological changes in L. pneumophila on the ultrastructural level has been studied. Disturbances in the ultrastructure of L. pneumophila result from the direct bactericidal action of interferons without any interference of immune mechanisms. These disturbances are manifested by damages in the cell wall, plasma membrane, nuclear and ribosomal apparatuses of microbial cells. Leukinferon exhibits pronounced anti-Legionella activity, both in vitro in a liquid culture medium and in ovo, than reaferon.

Animals↗
PubMed1990

[The isolation and characteristics of long-term continuous lymphoid cell lines (IK-10 and IK-12) from patients with mycosis fungoides].

Examinations of 26 cell cultures have detected in two cases continuous IK-10 and IK-12 cell lines from patients with mycosis fungoides. Studies of these cell ultrastructure, carried out by transmission electron microscopy, have shown organoids of all types, that are characteristic of both minor and medium lymphocytes and of lymphoblasts. Markers of T-, B-cells and mononuclears are also detectable in these cell lines. The cells express surface immunoglobulins and interleukin-2 receptors. Noteworthy that association of these cells in a population may be in vitro cultivated for more than 2 years.

Cell Line↗
PubMed1990

[A clinico-experimental study of patients with mycosis fungoides and Kaposi's sarcoma].

Twenty-six cultures were prepared by the cultivation method. Continuous cells lines (IK-10 and IK-12) were obtained in two cases, in the rest short-lived cell cultures were prepared. All the isolated cell cultures were studied by transmission electron microscopy. Abnormal lymphoblasts were detected in some cell cultures isolated from the blood and lymph nodes of mycosis fungoides (IJ-137, IK-140, IK-142, IK-143) and Kaposi's sarcoma patients (IK-145). Cell population consisted of small, medium, and large lymphocytes. Electron microscopic examination has revealed retroviruses in IK-143 culture, isolated from the lymph node of a patient with mycosis fungoides. Morphologically these particles were typical of type C oncoviruses, 120-140 nm in diameter, with a spherical core in the center of the virion.

Adolescent↗
PubMed1989

[The molecular bases of the pathogenesis of AIDS].

The comparative study of the amino acid sequence of gp120 in human immunodeficiency virus (HIV) strains HTLV-III and ARV-2 and the amino end areas of the growth hormone receptors of human skin and the insulin receptors has been carried out, thus making it possible to predict the existence of two compact domains connected with an area of a peptide chain. This area is incapable of the formation of a compact globular structure due to a high content of the remnants of proline. The data obtained as the result of electron microscopic study in combination with image processing have confirmed the predicted three-dimensional structure of gp120. This study has also shown that the amino acid sequence of some regions in the domains of gp120 has a significant degree of homology with similarly located regions of the growth hormone and insulin receptors; in its turn, this amino acid sequence is homologous to the framework regions of the VH domain of immunoglobulin. Antibodies to this VH domain specifically react with recombinant HTLV-III antigen. On the basis of the data obtained in our experiments and from the analysis clinico-immunological information, we have come to the conclusion that AIDS is an autoimmune disease induced by HIV due to the structural homology of gp120 with highly important receptors of human cells.

Acquired Immunodeficiency Syndrome↗
PubMed1989

[The morphological characteristics of the chondriome of human lymphoblastoid cells producing the human immunodeficiency virus].

The mitochondrial complex condition of continuous CEMT4 cell line infected by the human immunodeficiency virus has been investigated. The mitochondrial morphology of these and of intact cells was similar in great extent, though several changes were observed. For example, mitochondrial profiles with multiple dichotomous branches and anastomosis cristae were noted in the former. These changes resulted in the augmentation of the inner membrane square of mitochondrion. The formation of mitochondrial clusters connected with special junctions was a very characteristic part of the infected cell. Contacts were seen to be formed between the outer membranes neighboring profiles. These contacts look as X-like little bridges, or net-like or plate-like structures. The mutual transition of all these structures was observed using goniometer adapter. As has been shown by the three-dimensional reconstruction of mitochondrial junction zones, this area is presented by a single mitochondrion being structurally very complicated and very large in size compared to the neighbouring ones.

Cell Line↗
PubMed1989

[Electron microscopic analysis of the effect of Trichophyton rubrum on the reproduction of the human immunodeficiency virus].

The authors have examined the effect of a Tr. rubrum infection on HIV reproduction in H9 cell culture. A statistically significant activation on HIV multiplication has been observed 24 hrs after cell culture infection with the fungus: the infected cells produced more viral particles than the reference cells. The number of HIV virions has been increased mainly at the sites of Tr. rubrum localization and near H9 cell sites containing phagocytized elements of the fungus. In case of a mixed infection abnormal HIV forms (minimal and giant ones) have been detected along with the common virions. The ability of HIV virions to adsorption on Tr. rubrum surface has been revealed.

Acquired Immunodeficiency Syndrome↗
PubMed1978

[Alterations of Rous virus and polyoma virus during their synthesis in the cells of unnatural hosts].

The line of human embryo fibroblasts transformed by Rous sarcoma virus (strain Schmidt-Ruppin) contained and produced Rous sarcoma virus; this was shown by the complement fixation test, immunofluorescent test, electron microscopy and labelling with 3H-uridine peak in sucrose gradient. Biological properties of the new synthesized virus differed from that of the parent Schmidt-Ruppin strain; the range of the sensitive cells and the protein envelope antigens altered in particular. Analogous results by the change of the viral biological properties produced in the unnatural host tissue were obtained for polioma virus synthesized in the human embryo fibroblasts transformed by this virus.

Antigens, Viral↗
PubMed1978

[Ultrastructure of the conjugates of cytolytic T-lymphocytes and target cells].

Conjugates of target cells and of cytological T-lymphocytes obtained on the 11th day after alloimmunization were investigated. The conjugates formed small and medium lymphocytes; mature secretory granules, crystal-like structures and lipids were revealed in their cytoplasm. The lymphocyte is spherical, the area of contact with the target cell does not exceed 5 to 15%. Cytolysis of target cells is observed after 30 to 60 minutes of incubation. The lymphocyte becomes flattened, its nucleus acquires an oval form, and the area of contact with the target cell increases considerably. At the same time hypertrophy and change of orientation of Golgi's complex to the area of contact with the target cell, coalescence of the secretory granules with lipids and crystal-like structures, the appearance of immature secretory granules and vacuolar degeneration of mitochondria are demonstrated. The lymphocyte membrane becomes "desquamated"; structures connected with it, named "membranosomes" are described. It is suggested that the secretory processes in the cytoplasm of cytolytic T-lymphocytes are activated in their interaction with target cells.

Animals↗
PubMed1977

[Electron microscopic study of the interaction between cytolytic T-lymphocytes and target cells].

Thymocytes stimulated in vitro in a mixed culture was sorbed by centrifugation on the surface of target cells for electron microscope study of cytology of immune T-lymphocytes and early cytolysis periods. A well developed Golgi apparatus was revealed in the cytoplasm of lymphocytes; there was also accumulation of tubular structures 50 to 60 nm in diameter which communicated with the cysterns of the granular endoplasmic reticulum, with "descended" vesiculi and plasmatic membrane of lymphocyte. This membrane formed numerous contacts with the membrane of target cells thus producing closed clefts. Taking into consideration these data and also current views on the intertransformation of membranes and intracellular transport a hypothetic scheme of the mechanism involved in the cytolysis of the target cell by immune T-lymphocyte was put forward.

Animals↗
PubMed1977

[Electron microscopic study of the usual and "minimal forms" of oncornaviruses in human embryonic lung cells cataminated with mouse cells].

Electron microscopic examinations of KLChKM (human embryo cells spontaneously contaminated with mouse cells) cell cultures revealed extracellular oncornaviruses of A and C types and intracisternal virions of A type which in their submicroscopical organization and morphogenesis were identical to oncornaviruses of mouse L cells. Also "minimal forms" (MiF) of oncornaviruses of A and C types the diameter of which was 30--70 nm were found. MiF of oncornaviruses were found to be formed in the process of budding through the cytoplasmic membrane or membranes of intracellular vacuoles.

Cells, Cultured↗
PubMed1975

[Comparative study of the ultrastructure of immune and normal phytohemagglutinin-treated lymphocytes].

Immune lymphocytes sorbed on the surface of the target cells were characterized during the period of the first three hours of combined incubation by the presence of the electron-dense matrix, abundance of mitochondria and lipids; small lymphocytes had disseminated ribosome organized into polysomes in the medium lymphocytes forming individual cysterns of the granular endoplasmic reticulum in the large lymphocytes, this indicating active protein synthesis by these cells. There were also revealed cells of plasmatic type. Cells incubated with the PHA for one hour represented a homogenous population of small lymphocytes of the same size as the clear cytoplasm containing free ribosomes and individual mitochondria. The proportion of the medium lymphocytes and the blasts increased with increase of the incubation period. These are cells with the clear cytoplasm freely disseminated polyribosomes in which no developed granular endoplasmic reticulum was sometimes revealed. The presence of two types of cells whose ultrastructure reflected their functional characteristics is discussed.

Animals↗
PubMed1975

[Elimination of large pyroninophilic cells due to the effect of phytohemagglutinin].

Large pyroninophilic lymphocytes adsorbed on the surface of target-cells disappeared after phytohemagglutinin (PHA) addition. Incubation during 45 minutes in the presence of PHA did not reveal cisternas of the granular endoplasmatic reticulum and the number of mitochondrias decreased. The H3-thymidine-labeled cells were almost eliminated. There appeared population of small lymphocytes with even outline and clear cytoplasm, poor in organellas, with ribosomas freely scattered in it. After 24--48 hours of incubation they transformed into blasts, large cells with clear nucleus and clear cytoplasm in which no cisternas of granular endoplasmatic reticulum were revealed.

Animals↗