PubMed Health⌕ Search

Biomedical subjects

A F Farid

Publications and source records attributed to A F Farid.

11 recordsLinked to original sources

A comprehensive study of environmental and human pathogenic Vibrio alginolyticus strains.

An investigation comprising 49 environmental (VAE), 7 human isolates (VAH) and 3 type culture (VAT) strains of Vibrio alginolyticus showed a very high similarity among the strains. By primary isolation a swarming activity on blood agar and a yellow, hemispheric relatively large colony type on TCBS were very conspicuous. Besides the characteristics of Vibrionaceae the most typical and differential diagnostic important features were: sucrose fermentation, lysine decarboxylase activity, acetoine and 2,3 butanediol production, no growth on an electrolyte deficient medium (CLED), arginine and arabinose negative reaction. Swarming on blood agar and growth in 10% NaCl were also important although one of these criteria might be negative. An obvious difference was observed in ornithine where the percentage of positive VAE, VAH and VAT were 51, 100 and 0, respectively. In cellobiose all VAE and VAT but only two VAH were positive. MR positive strains were also found among all categories. The G + C mol% in the strains ranged from 45.9 - 46.8.

Amino Acids↗

Occurrence of Vibrio parahaemolyticus and Vibrio alginolyticus in marine and estuarine bathing areas in Danish coast.

From seventeen sites in three marine and two estuarine bathing areas at the Danish coast water samples were collected during the period from May to November 1979. Total bacterial counts were performed on blood agar. The prevalence of Vibrio parahaemolyticus and Vibrio alginolyticus in 100 ml water samples using the filter membrane technique and TCBS agar plates was investigated with the possible correlation to water temperature and salinity. Only five strains of V. parahaemolyticus were isolated, two from marine and three from estuarine water. V. alginolyticus was isolated in 8.33 and 15.63 per cent, in relation to the total bacterial count/100 ml on TCBS agar, in marine and estuarine water samples, respectively. The maximum was registered in June and July, corresponding with the highest temperatures recorded (12-18 degrees C). V. alginolyticus should be considered a widely distributed bacterium in Danish coastal areas and could be regarded as a bacterial indicator of hygienic and public health importance.

Bathing Beaches↗

In vitro antibiotic sensitivity testing of Vibrio alginolyticus.

V. alginolyticus from environmental sources of different geographical areas and some human pathogenic strains were investigated for their sensitivity to 29 different antimicrobial agents, using the agar diffusion method. All strains were sensitive to Gentamicin, Neomycin, Sulfaisodimidin, Sulfamethoxazole-Trimethoprim, Rifamycin, Nalidixan and Linco-Spectin and all except one to Trimethoprim, Tetracycline, Chloramphenicol, Nitrofurantoin and Tobramycin. Between 80 and 94% of tested strains were sensitive to Clindamycin, Cephalotin, Kanamycin, Tylosin and Spiramycin, while Ampicillin, Vancomycin and Novobiocin were effective to approximately 50%. A high percentage of resistance was found to Meticillin, Carbenicillin and Lincomycin. All strains were resistant to Penicillin and Fucidin. No variation in sensitivity pattern between environmental strains isolated from different areas was detected. Great correlation was found among environmental and human pathogenic strains, which could be helpful as guidance in therapy.

Anti-Bacterial Agents↗