Biomedical subjects

A F Frost

Publications and source records attributed to A F Frost.

4 recordsLinked to original sources

PubMed1983

Dominant lethal effects of n-butyl glycidyl ether in mice.

Using the dominant lethal assay, the ability of n-butyl glycidyl ether to induce mutations in male mice was investigated. No significant dose-related changes either in pregnancy rates or in average number of implants per pregnant female were found. However, while the results were not altogether conclusive, there was evidence of a significant increase in fetal death rates by the end of the first week after the highest dosage was administered.

Animals↗

PubMed1982

Unscheduled DNA synthesis induced in human lymphocytes by butyl glycidyl ethers.

Unscheduled DNA synthesis (UDS) was examined in normal human peripheral blood lymphocytes (HPBL) exposed to n- and tert.-butyl glycidyl ethers (BGE). n-BGE, which is currently used as a major reactive diluent for industrial epoxy resin systems, has been implicated as a bacterial mutagen. t-BGE, a potential replacement for n-BGE, is less cytotoxic to HPBL. Both these BGEs have demonstrated genetic activity in human cells. Here, slide autoradiography and liquid scintillation counting techniques have been used to show that both n-BGE and t-BGE induce repair-DNA synthesis in normal HPBL. These agents demonstrate similar dose-response relationships over the range of concentrations up to 333 micrograms/ml. Cultures were examined for repair-DNA synthesis after a continuous 5-h exposure to varying concentration of n-BGE or t-BGE.

Cell Survival↗

PubMed1978

Cell interaction during lymphocyte activation.

Adherent cells were separated from stimulated lymphocytes by a layer of agarose to determine the requirement for lymphocyte interaction with adherent cells. The results demonstrated that the presence of plastic adherent cells was obligatory for oxidative activation, but contact of plastic adherent cells with oxidized nonadherent lymphocytes was not. A similar relationship was found to exist between adherent and nonadherent populations during PHA and Con A stimulation. In contrast, the responses of PWM- and ZnCl2-stimulated lymphocytes were not augmented by the presence of adherent cells unless the two populations were in contact.

Cell Adhesion↗

PubMed1976

Stimulation of human peripheral blood lymphocytes by a sodium periodate (NaIO4) induced growth factor.

Sodium periodate treated lymphocytes engage in cell to cell interaction and also yield a soluble growth factor that stimulates DNA synthesis when added to naive autologous cells. Adherent cells enhance the factor mediated activity. Sodium periodate stimulated lymphocytes, treated with mitomycin C, produced no growth factor activity. Partial characterization of the factor indicates that it is non-dialyzable, resistant to ribonuclease, and sensitive to heat, trypsin, and papain.

Cells, Cultured↗