A simple microassay for detection of antibodies to fetal calf serum and related antigens and its application to the serological definition of human tumor antigens.
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Animals↗
Biomedical subjects
A F Rahman
Publications and source records attributed to A F Rahman.
Characterization of human malignant melanoma cell lines. VII. Glycoprotein synthesis and shedding as revealed by [3H]glucosamine labeling.
We have established conditions for the study of membrane glycoprotein synthesis and turnover in cultured human malignant melanoma cell lines using the labeled precursor [3H]glucosamine. Uptake of label increased parallel with cell growth, reaching a steady state in resting cultures. Fifteen to 30% of incorporated label can be released from the cells by trypsin treatment depending on the conditions of exposure to the enzyme, and about 50% of the incorporated label is spontaneously shed from the cells within 96 hr of incubation. Labeling in exhausted medium gave a 5- to 8-fold increase in uptake which was inhibited by addition of glucose (2 mg per ml) into the culture medium. The percentage of trypsin-releasable material was identical in fresh and exhausted medium; however, the percentage shed was less in cells initially labeled in exhausted medium. These data provide background information for further studies on the antigenic composition of the glycoproteins of cultured melanoma cells.
Cell Division↗
Letter: 'Bacteruritest' strips.
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Bacteriological Techniques↗
Comparison of the sensitivity of human embryo kidney cells, HeLa cells, and WI38 cells for the primary isolation of viruses from the eye.
A comparison has been made of the efficiency of human embryo kidney (HEK) cills, HeLa cells, and WI38 cells for the isolation of viruses from the eyes of patients suffering from acute conjunctivitis or keratoconjunctivitis. From a total of 99 specimens 21 adenoviruses (serotypes 3, 4, 7, 8, and 13) were isolated in HEK cells, eight (serotypes 3 and 8) in HeLa cells, and four (serotype 3) in WI38 cells. Of the ten herpes simplex viruses isolated nine were recovered in HEK cells, seven in WI38 cells, and none in HeLa cells. The combination of HeLa cells and WI38 cells is not considered an adequate alternative to the difficult-to-obtain HEK cells for the isolation of viruses from the eye.
Adenoviridae↗
Rat immunoglobulins in serum and secretions: comparison of IgM, IgA and IgG in serum, colostrum, milk and saliva of protein malnourished and normal rats.
Immunoglobulin levels in colostrum and milk of normal and protein malnourished rat dams and in serum and saliva of their offspring were quantitated by radial immunodiffusion employing purified anti-rat alpha, gamma and mu. In general, no significant difference was observed in the level of colostral IgA in malnourished as compared to normal rat dams or in the level of serum IgA and IgM and salivary IgA and IgG2a of their offspring. On the other hand, malnourished mothers demonstrated approximately twofold lower levels of colostral IgG2a than normal mothers throughout the entire period of lactation. In addition, at every interval tested, the level of IgG was approximately 1.5- to 2-fold less in malnourished as compared to normal offspring.
Animals↗
Effective immunity to dental caries: protection of gnotobiotic rats by local immunization with Streptococcus mutans.
Gnotobiotic rats injected in the submandibular region with killed, whole Streptococcus mutans cells developed salivary antibodies directed to this microorganism. Increased levels of salivary IgA and inhibition and augmentation of agglutinin titers with anti-rat alpha-antiglobulin suggested that these antibodies were of the immunoglobulin A class. Furthermore, the rats monoinfected and immunized with homologous organisms always had lower mean caries scores than monoinfected, non-immunized rats. This reduction was evident in carious lesions on the buccal surfaces of molars and in those in sulcal areas. These results suggest that local immunization with whole S. mutans cells stimulates a specific salivary IgA response protective against caries resulting from S. mutans infection.
Agglutination Tests↗