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Biomedical subjects

A F Voevodin

Publications and source records attributed to A F Voevodin.

28 records · Page 2Linked to original sources
PubMed1979

Prevalence of antibodies to soluble antigen of Epstein-Barr virus-producing cells (P3HR-1) in the sera of hamadryas baboons of the high lymphoma incidence stock.

Soluble antigen of P3HR-1 cells (SA-P3HR-1) was identified in indirect double immunodiffusion enhanced with tannic acid using serum of a nasopharyngeal carcinoma patient containing high-titer antibodies to Epstein-Barr virus (EBV) antigens. SA-P3HR-1 was nonidentical to soluble antigen of Raji cells. Human and baboon sera containing antibodies to all the known antigen of EBV and HVP respectively were anti-SA-3HR-1-positive. Human and baboon sera containing antibodies to all the known antigens of EBV and herpesvirus Papio (HVP) were also anti-SA-P3HR-1-negative. Prevalence of anti-SA-P3HR-1 was very high in two groups of the high-lymphoma incidence stock of hamadryas baboons of the Sukhumi monkey colony. 54% (15 of 28) and 38% (13 of 34) of clinically lymphomatous and clinical normal monkeys, respectively, were anti-SA-P3HR-1-positive.. Only 1 of 30 normal baboons studied, living in the forest and having no contacts with the baboons in the main stock of the Sukhumi monkey colony, was anti-SA-P3HR-1-positive (3%).

Animals↗
PubMed1978

Identification of the soluble HVP-associated antigen of the lymphoblastoid cell line established from lymphomatous baboon (Papio hamadryas).

A new technique (indirect double immunodiffusion) for detection of EBV-associated soluble antigen and corresponding antibodies has been developed. This technique includes three steps: 1) simple double immunodiffusion with extracts of Raji cells (or other EBV-genome positive cells) and human sera containing antibodies against EBV-associated soluble antigen; 2) extensive washing and treatment with anti-human globulin; 3) extensive washing and treatment with tannic acid. Using this test it was shown that the soluble antigen indistinguishable from EBV-associated soluble antigen was present in KMPG-1 cells producing HVP.

Animals↗
PubMed1976

Antigenic characterization of c-type oncornavirus isolated from lymphomatous baboon (Papio hamadryas).

Antigens of BILN C-type oncornavirus isolated from lymphomatous baboon were studied in the double immunodiffusion. The major viral protein (P-30) of this virus possesses interspecies antigenic determinants (GS-3). This protein also revealed close antigenic relation to the P-30 of RD-114 and M-7 C-type oncornaviruses. Specific serum against BILN P-30 has been produced. The comparison of the three test-system (P-30s of M-7, RD-114, and BILN, and specific sera against them) did not allow to distinguish these proteins by the method used.

Animals↗
PubMed1975

Transmission of human leukaemia to nonhuman primates.

The experiments described show that inoculation of two monkey species, M. arctoides and P. hamadryas, with human leukaemic blood or its filtrates causes a viral disease with the characteristics of malignant lymphoma of mixed type. The disease was passed in subsequent passages. The virus was isolated and identified as an oncornavirus of C-type by its characteristic morphological appearance and buoyant density (1.16 g/cm3 in sucrose and 1.21 g/cm3 in caesium chloride), and by the presence of 60-70S RNA and RNA-dependent DNA polymerase. There is some evidence that it differs immunologically from other known oncornaviruses of mammals including primates. It is postulated that both horizontal and vertical transmission of this oncornavirus can occur.

Adolescent↗
PubMedDate not supplied

[Antigenic characteristics of oncovirus persisting in continuous Syrian hamster cell culture].

The antigenic properties of an oncornavirus persisting in a continuous Syrian hamster cell culture (X-100) were studied. By the mutual immunodiffusion test it was shown that virions and virion-producing cells contained a protein antigenically identical to the main structural protein of oncornaviruses type C of Syrian hamsters. The materials tested contained no "GS-1" antigens (species-specific antigenic determinants of the main structural protein of mammalian oncornaviruses type C) of murine, feline, simian viruses or group-specific antigens of Bitner and Mason-Pfiser viruses.

Animals↗
PubMedDate not supplied

[The isolation and characteristics of the green monkey lentivirus].

We characterized the simian immunodeficiency virus isolated from Cercopithecus aethiops (subspecies C. a. pygerythrus) originating from Kenya. SIV was isolated and continuously produced with the MOLT4 clone 8 cell line and was designated as SIV-SU1. SIV-SU1 isolate replicated with high efficiency in MOLT4 clone 8, MT-2 with moderate efficiency in CEM x 174 and with poor efficiency in HUT-78, U937, C8166. The infection of MT-2, C8166 and HUT-78 resulted in extensive cell killing. Western blotting of purified preparations of SIV-SU1 revealed viral proteins of 130, 68, 55, 41, 24, 17 kDa. Cross-reactivity of SIV-SU1 proteins with HIV-1, HIV-2, SIVmac, SIVsm, SIVmnd was studied by radioimmunoprecipitation assay. The most extensive cross-reactivity was observed with SIVmac. Total cellular DNA from chronically infected cells was hybridized to SIVagm266 DNA probes. Detection of cross-hybridizing DNA sequences required very low stringency, and the restriction endonuclease fragmentation pattern of SIV-SU1 differed from other SIVs.

Animals↗