PubMed Health⌕ Search

Biomedical subjects

A F Yassin

Publications and source records attributed to A F Yassin.

28 records · Page 2Linked to original sources
PubMed1997

Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei.

Data from chemotaxonomic and 16S ribosomal DNA sequence analyses of an isolate obtained from the sputum of a kidney transplant patient identified the isolate as a member of the genus Nocardiopsis. DNA-DNA hybridization data, as well as physiological characteristics, indicated that the isolate represents a new species of the genus Nocardiopsis, designated Nocardiopsis synnemataformans; the type strain is strain IMMIB D-1215 (= DSM 44143). In addition, DNA-DNA hybridization data, as well as the results of biochemical tests, indicated that Nocardiopsis alborubida DSM 40465T, Nocardiopsis antarctica DSM 43884T, and Nocardiopsis dassonvillei DSM 43111T represent a single species designated N. dassonvillei. We also found that Nocardiopsis alba subsp. alba DSM 43377T and N. alba subsp. prasina DSM 43845T are genetically different and therefore propose that N. alba subsp. prasina be elevated to species status as Nocardiopsis prasina comb. nov., whose type strain is strain DSM 43845.

Base Composition↗
PubMed1996

Rapid chemotaxonomic diagnosis of human tuberculosis.

65 different clinical specimens from patients suspected of being infected with Mycobacterium tuberculosis were examined by three different diagnostic methods. Two of these methods were the conventional microscopic and cultural examinations. The third, a modern chemotaxonomical method is based upon the detection of tuberculostearic acid by GC-MS analysis using selected ion monitoring (GC-MS/SIM). Comparison of the results of the GC-MS analysis with those of the conventional methods has indicated that tuberculostearic acid analysis can be used for diagnosing tuberculosis under diagnostic routine conditions. The GC-MS method is rapid, usually providing results within 20 hours or less.

Gas Chromatography-Mass Spectrometry↗
PubMed1996

Tsukamurella pulmonis sp. nov.

Chemotaxonomic and 16S ribosomal DNA sequence analyses of an isolate from the sputum of a patient with a mycobacterial lung infection clearly delineated a new species of the genus Tsukamurella. This new species can be defined on the basis of genotypic and phenotypic data. The name Tsukamurella pulmonis sp. nov. is proposed for this organism; the type strain is IMMIB D-1321T (= DSM 44142T). This isolate shows 44.2 and 36.2% DNA relatedness to Tsukamurella paurometabola DSM 20162T (T = type strain) and Tsukamurella inchonensis DSM 44067T, respectively.

Aged↗
PubMed1995

Lentzea gen. nov., a new genus of the order Actinomycetales.

We describe a new genus of mesophilic actinomycetes, for which we propose the name Lentzea. The strains of this genus form abundant aerial hyphae that fragment into rod-shaped elements. Whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar (wall chemotype III). The phospholipid pattern type is type PII (phosphatidylethanolamine is the characteristic phospholipid); the major menaquinone is MK-9. The fatty acid profile comprises saturated, unsaturated, and branched-chain fatty acids of the iso and anteiso types in addition to tuberculostearic acid (10Me-C18:0). A 16S ribosomal DNA sequence analysis revealed that the genus Lentzea is phylogenically related to the genera Actinosynnema, Saccharothrix, and Kutzneria. The type species of this genus is Lentzea albidocapillata sp. nov.; the type strain of this species is strain IMMIB D-958 (= DSM 44073).

Actinomycetales↗
PubMed1995

Tsukamurella inchonensis sp. nov.

Chemotaxonomic and genomic 16S ribosomal DNA sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus Tsukamurella. This new species can be identified by its 16S ribosomal DNA similarity values, as well as its physiological characteristics. The name Tsukamurella inchonensis sp. nov. is proposed for these isolates, which are represented by strain IMMIB D-771T (= DSM 44067T) (T = type strain). This strain exhibits only 45% DNA relatedness to Tsukamurella paurometabola.

Actinomycetales↗
PubMed1993

Identification of mycobacterial isolates by thin-layer and capillary gas-liquid chromatography under diagnostic routine conditions.

The mycolic acid patterns of 75 strains of mycobacteria belonging to 46 different species were studied by thin-layer chromatography (TLC). Additionally, the mycolic acid pyrolytic cleavage products were determined by capillary gas-liquid chromatography (C-GLC). Eleven different patterns based on number, type and Rf values of the detected lipid spots were identified. The mycolic acid methyl ester profiles of clinical isolates were compared with those of reference strains. In this way, it was possible to relate these clinical isolates to mycobacterial groups defined by their mycolic acid profiles. The 11 patterns and the methods used are described in detail.

Chromatography, Gas↗
PubMed1993

Cellular fatty acid methyl ester profiles as a tool in the differentiation of members of the genus Mycobacterium.

The cellular fatty acid profiles of 84 strains belonging to 53 different species of the genus Mycobacterium were determined by gas liquid chromatography-mass spectrometry (GCMS). Two main types and four subtypes of fatty acid profiles were recognizable. The first main type is the G type, nominally referring to M. gordonae, members of which lack tuberculostearic acid or other 10-methyl branched-chain fatty acids, but contain normal saturated and unsaturated fatty acids. This type is further subdivided into the G alpha subtype that is characterized by 2-methyl tetradecanoic acid (2-Me-C14:0) as the only 2-methyl branched fatty acid. Strains belonging to the second main type, the T type, nominally referring to tuberculosis, contain tuberculostearic acid and other 10-methyl branched acids in addition to the normal saturated and unsaturated ones. This type has been further subdivided into three subtypes: the T alpha subtype that does not contain any 2-methyl branched fatty acids; the T beta subtype that contains both 2-methyl tetradecanoic (2-Me-C14:0) and 2,4-dimethyl tetradecanoic (2,4-DMe-C14:0) acids as 2-methyl branched fatty acids; the T gamma subtype which contains 2-methyl dodecanoic (2-Me-C12:0), 2,4-dimethyl dodecanoic (2,4-DMe-C12:0) and 2,4-dimethyl tetradecanoic (2,4-DMe-C14:0) acids as 2-methyl branched-chain acids. Fatty acid analysis showed a great homogeneity within the genus and the profiles produced were not very helpful in distinguishing between members of the genus Mycobacterium except for the identification of M. gordonae, M. kansasii, and M. gastri.

Chromatography, Gas↗
PubMed1991

Menaquinone patterns of Amycolatopsis species.

The menaquinones of representative strains of the actinomycete genus Amycolatopsis were examined by reverse phase thin-layer chromatography and mass spectrometry. Representatives of all seven validly described species contained various combinations of di-, tetra- and hexahydrogenated menaquinones with nine isoprene units as predominant isoprenologues. It seems likely that the variation in the predominant menaquinones merely reflects the stages from the growth cycle from which biomass was taken. The detection of major proportions of hydrogenated menaquinones with nine isoprene units serves to distinguish Amycolatopsis strains from most other actinomycetes, notably those belonging to related genera such as Amycolata and Pseudonocardia.

Actinomycetales↗
PubMed1988

Menaquinone composition in the classification and identification of aerobic actinomycetes.

Menaquinones were the only isoprenoid quinones found in 36 strains representing different species of the genera Nocardia, Mycobacterium, Rhodococcus, Amycolatopsis, Saccharothrix, Streptomyces, Nocardiopsis and Actinomadura. Dihydrogenated menaquinones with nine isoprene units [MK-9(H2)] were the main components isolated from Mycobacterium. Dihydrogenated and tetrahydrogenated menaquinones with eight isoprene units were the predominant compounds identified in typical Rhodococcus and Nocardia strains, respectively. "Nocardia phenotolerans" differed from all of the other Nocardia species included in the study, in that it contained the MK-9(H2) [MK-8(H2)] menaquinone system. Nocardioform bacteria lacking mycolic acids contained tetrahydrogenated menaquinones with nine isoprene units as the main component. The Streptomyces strains studied exhibited complex mixtures of partially saturated menaquinones with nine isoprene units with the hexa- and/or octahydrogenated components predominating. Actinomadurae contained major amounts of hexahydrogenated menaquinones with nine isoprene units. In contrast, the single Nocardiopsis strain examined possessed complex mixtures of menaquinones with ten isoprene units, the dihydrogenated components being main constituents.

Actinomycetales↗