Biomedical subjects

A F de Carvalho

Publications and source records attributed to A F de Carvalho.

9 recordsLinked to original sources

PubMed2001

Harmonisation and dosimetric quality assurance in individual monitoring for external radiation.

The current situation amongst Member States is that there are widely differing national requirements for dosimetric services and for dosemeter performance. It is clear that with the free movement of workers within the European Union (EU) and the requirements for individual dosimetry given in Council Directive 96/29 EURATOM, a degree of harmonisation of requirements and procedures of EU Member States would be desirable. A EURADOS action group, made up of members from each of the EU Member States plus Switzerland, was set up with the overall objectives of consolidating within the EU the quality of individual monitoring using personal dosemeters and assisting movement towards harmonised procedures. An outline of the work of the action group is given and the term 'harmonisation' is discussed.

Europe↗

PubMed2001

Present status of approval procedures in the EU member states and Switzerland.

The European Union Council Directive 96/29/EURATOM requires that 'individual monitoring shall be ... based on individual measurements which are established by an approved dosimetric service' and that 'Each Member State shall make arrangements to recognise, as appropriate, the capacity of ... approved dosimetric services'. At present, approval of dosimetric services does not have the same meaning within EU Member States and Switzerland. In some countries, service and dosemeter approval is clearly separated, in some others only one of the two is supposed to be tested, and in others no approval is required. Dosimetric requirements and criteria are based on different international documents (e.g. IEC, ISO, ANSI, CEC report) or national specific rules. Approval frequency can be once, every 2 or more years. Approval can be based on either evaluation of technical and management reports, irradiation tests, inspection on-site or the three steps together. In most cases, approval involves photon dosimetry while beta and neutron dosimetry test procedures are not as well established. However, comparisons may lead to some convergent evolution of procedures and to a greater degree of harmonisation and quality consolidation.

European Union↗

PubMed2000

NABC1 (BCAS1): alternative splicing and downregulation in colorectal tumors.

We have identified a new splicing variant of the gene "novel amplified in breast cancer 1," NABC1 (HGMW-approved symbol BCAS1). This variant, which we call NABC1_5B, uses a previously unidentified 135-bp exon. Also in this report, we confirm that NABC1 is overexpressed in breast tumors and show that both NABC1 and NABC1_5B are downregulated in colorectal tumors.

Alternative Splicing↗

PubMed1998

Lipid accumulation in obese Zucker rats is reduced by inclusion of raw kidney bean (Phaseolus vulgaris) in the diet.

The effects of inclusion of different levels of raw kidney bean (Phaseolus vulgaris) of high lectin content (27 g/kg meal) in a high-quality (lactalbumin) control diet were tested in nutritional trials on the growth and metabolism of obese Zucker (fafa) rats and their lean littermates in comparison with pair-fed controls. All diets contained 100 g total protein/kg and either 50 g lipids/kg (low fat) or 150 g lipids/kg (moderate fat). The growth of both obese and lean rats on bean diets was retarded by the daily bean intake in a dose-dependent manner. However, most of this was because bean-fed rats contained less body fat than the controls after 10 d. Thus, after feeding low-fat diets containing up to 130 g kidney bean/kg (lectin intake < or = 0.2 g/kg body weight (BW) per d) in both 10 d and 70 d trials, the bodies of obese rats contained less fat but not protein than their pair-fed controls. Moreover, by increasing the lipid content of the diet to 150 g/kg, the level of bean inclusion could be increased to 280 g/kg (lectin intake > or = 0.4 g/kg BW per d) without loss of body protein and skeletal muscle. Although these rats contained more body fat than those which were fed on low-fat diets, their weight reduction could be accounted for exclusively by reduced lipid content. In contrast, significant body protein loss occurred when the same diet of high lectin content was fed to lean littermates. Plasma insulin levels were significantly depressed in the obese Zucker rats on bean diets but the pancreas was not significantly enlarged nor its insulin content changed in 10 d trials. However, significant pancreatic growth occurred on long-term (70 d) bean feeding compared with pair-fed controls. The results suggest that, in addition to animal nutrition, it may also be possible to use the bean lectin as a dietary adjunct or therapeutic agent to stimulate gut function and ameliorate obesity if a safe and effective dose-range can be established for human subjects.

Animals↗

PubMed1995

Reclassification of "Propionibacterium rubrum" as P. jensenii.

The taxonomic relationship of strains previously designated as "Propionibacterium rubrum" to P. thoenii and P. jensenii was investigated by use of 16S ribosomal RNA sequence comparison, biochemical characteristics and DNA hybridization. A total of 46 strains representing the species P. jensenii and P. thoenii and the former species "P. rubrum" and also including 21 reference strains and 25 strains isolated from dairy sources were studied. The 16S rRNA sequence of strain "P. rubrum" CNRZ 85 (= ATCC 4871) was found to be almost identical to that of the type strain of P. jensenii. DNA hybridization data indicated that "P. rubrum" should belong to the species P. jensenii rather than P. thoenii, as formerly proposed. The "P. rubrum" strains should then be reclassified as a beta-haemolytic biovar of P. jensenii. The genomic species P. jensenii and P. thoenii could be differentiated by biochemical characteristics such as the production of acid from myo-inositol and starch.

DNA, Bacterial↗

PubMed1992

Alterations in the level of insulin receptor and GLUT-4 mRNA in skeletal muscle from rats fed a kidney bean (Phaseolus vulgaris) diet.

1. A decline in the level of circulating insulin was observed in rats fed a diet containing kidney bean. 2. Consumption of a diet containing kidney bean caused an increase in the level of mRNAs for the insulin receptor (327%) and GLUT-4 (185%) in the gastrocnemius muscle. In contrast there was only a small increase in the amount of actin mRNA (125%). Since the kidney bean-fed rats are euglycaemic the results suggest that insulin receptor and GLUT-4 mRNA levels are regulated in response to circulating insulin concentrations rather than glucose. 3. No increases in the level of insulin receptor and actin mRNA were evident in the soleus muscle of rats fed the diet containing kidney bean; however a decline was observed in the level of GLUT-4 mRNA. 4. It is proposed that a component of kidney beans, most likely the lectin phytohaemagglutinin, has systemic effects which lead to changes in expression of the insulin receptor and GLUT-4 genes and to the sensitivity of muscle to insulin.

Actins↗

PubMedDate not supplied

Identification of diary Propionibacterium species by rRNA gene restriction patterns.

A total of 78 strains of dairy propionibacteria, 4 reference strains of Propionibacterium and 8 related bacteria were characterized by ribosomal ribonucleic acid (rRNA) gene restriction patterns (ribotyping). The patterns were obtained after cleavage of total DNA with either BamHI or ClaI restriction endonucleases and hybridization of fragments with acetylaminofluorene-labelled 16 + 23S rRNA from Escherichia coli. The four different species of dairy propionibacteria, P. freudenreichii, P. jensenii, P. thoenii and P. acidipropionici, gave different restriction patterns with species-specific fragments. Moreover, ribotyping allowed the differentiation of P. freudenreichii subsp. freudenreichii from P. freudenreichii subsp. shermanii. The patterns of dairy propionibacteria were different from those of closely related bacteria and other bacteria used in the dairy industry.

Bacterial Typing Techniques↗

PubMedDate not supplied

Syphilitic gumma.

Explore the source record for details and available documents.

Adult↗

PubMedDate not supplied

Biological activities of a human amniotic membrane interferon.

In order to characterize further the human amniotic membrane interferon (IFN-AM), an interferon antigenically unrelated to human IFN-alpha, -beta, and -gamma or TNF, we analysed its biological activities. Here, we present direct evidence of its ability to affect cell growth and to induce the IFN-stimulated genes (ISGs) 6-16 and 2'-5' oligoadenylate synthetase (OAS), in addition to its crossed anti-viral activity. The cellular growth arrest effect of IFN-AM was dose-dependent and paralleled that of IFN-beta. IFN-AM was also able to inhibit thymidine incorporation into DNA, similar to IFN-beta. The mRNA induction of 6-16 gene with IFN-AM treatment reached its highest level at 500 IU/ml and remained constant up to 2000 IU/ml. Conversely, 2'-5' OAS mRNA induction was dose-dependent, with the maximum level detected at 2000 IU/ml of IFN-AM treatment. The time course of mRNA accumulation by ISGs with IFN-AM (500 IU/ml) stimulation was also investigated. Gene induction reached a maximum at 16 h after IFN treatment for 2'-5' OAS and at 48 h for the 6-16 gene. IFN-AM and human IFN-alpha induced similar levels of the OAS enzyme. IFN-AM also showed small but significant activity in bovine cells. In conclusion, the amniotic membrane IFN here studied showed both anti-cellular activity and the ability to stimulate ISG-transcriptional activation in a similar manner to IFN-beta. In addition, IFN-AM was also as able to induce the expression of the enzyme 2'-5' OAS, as did IFN-alpha. Lastly, amniotic IFN showed a significant cross-species anti-viral activity, which was different from both human IFN-alpha and -beta. Taken together, these data strongly suggest that IFN-AM is a novel sub-type I IFN.

2',5'-Oligoadenylate Synthetase↗