A Fatou | PubMed Health

PubMed1990

Electrophoretic analysis of gene-enzyme systems in Chabertia ovina.

In Chaberia ovina species an electrophoretic study of 15 loci of the following enzymes has been conducted: glucose phosphate isomerase, mannose phosphate isomerase, glucose-6-phosphate dehydrogenase, glutamate-oxaloacetate transaminase, superoxide dismutase, isocitrate dehydrogenase, hexokinase, adenylate kinase, malate dehydrogenase, malic enzyme, carbonic anhydrase and 6-phosphogluconate dehydrogenase. The genetic variability has been relatively high, with 40% polymorphism values noted, an 0.10 mean heterozygosity observed and an 0.17 mean heterozygosity expected. The greater part of the allele frequencies were not in Hardy-Weinberg equilibrium.

Alleles↗

PubMed1988

Superoxide dismutase from Ascaris suum.

Three superoxide dismutases (SOD) (EC 1.15.1.1) were detected in homogenates of Ascaris suum. Each of the three forms of SOD was purified by a sequence of multiple differential centrifugations, ammonium sulphate precipitation, ion-exchange chromatography and G-75 Sephadex column chromatography. The three forms of SOD were present in different cellular locations; one in the cytoplasmic fraction, sensitive to cyanide and hydrogen peroxide, and two in the mitochondrial fraction, one of which was cyanide sensitive. The SOD forms presented clear differences in their electrophoretic patterns. The sexual organs of females showed the highest SOD activities of all the tissues examined. The finding of such high levels of superoxide dismutase in A. suum reflects the importance of this enzyme in the metabolism of this helminth parasite.

Animals↗