[Topographic relation between glutamic-oxalacetic transaminase and the different regions of electrical impairment of the heart].
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Aged↗
Biomedical subjects
A Fleury
Publications and source records attributed to A Fleury.
[Percussive symptomatology. New method].
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Humans↗
Detection of secreted cysticercal antigen: a useful tool in the diagnosis of inflammatory neurocysticercosis.
Neurocysticercosis is a common parasitic disease of the human central nervous system. It is particularly prevalent in developing countries, where it has a serious public health and economic impact. A major diagnostic problem with neurocysticercosis is its pleomorphic nature. Conventional diagnosis of neurocysticercosis still requires brain-computed tomography and/or magnetic resonance imaging, which are definitive but often prohibitively expensive and inaccessible in endemic areas. Herein, the monoclonal antibody HP10 antigen-trapping enzyme-linked immunosorbent assay, which has been used successfully to detect viable Taenia solium cysticercosis, was evaluated using cerebrospinal fluid (CSF) from Mexican neurocysticercosis patients with various defined pathologies. Sensitivity was higher in cases of inflammatory compared with non-inflammatory disease (94.1% vs. 33.3%) and in cases of multiple- compared with single-cyst cysticercosis (85% vs. 33.3%). Positivity was a strong indicator of active, inflammatory, multiple-cyst neurocysticercosis detecting 100% (15/15) of such cases. The overall specificity, as determined using CSF samples from patients with other neurological symptoms, was 97.7% (42/43). Since the assay only detects viable infection, it is of known value in the follow-up of treated patients to determine whether treatment has been successful. Thus, antigen detection may be of particular value in the assessment of symptomatic patients, who may potentially benefit from rapid treatment.
Animals↗
High prevalence of calcified silent neurocysticercosis in a rural village of Mexico.
Human neurocysticercosis (NC) is a parasitic disease caused by TAENIA SOLIUM when its larvae lodge in the central nervous system. NC prevalence estimates are obscured by the variable and often asymptomatic clinical picture. While infection depends on exposure, severity is possibly related with various host factors (immunity, genes and gender). This epidemiological study of cranial CT scans in an endemic rural community found that 9.1% of apparently healthy subjects had calcified lesions and were completely asymptomatic. Silent NC cases did not correlate with the exposure factors tested but showed family aggregation and higher rates of positive serology. Thus, NC prevalence may be higher than currently considered and host-related factors appear to be involved in infection and pathogenesis.
Adolescent↗
Studies of the guinea pig adrenal cytochrome P450c17 cDNA.
Cytochrome P450c17 is a single enzyme that catalyzes two successive reactions within the delta 5 and delta 4 pathways. The proteins expressed with human, bovine, and rat cDNAs convert both pregnenolone and progesterone into delta 5-delta 4-C19 steroids, although the rat cDNA prefers the delta 4 pathway. Our results showed that the guinea pig adrenal possesses the enzymatic machinery to produce C19 steroids and suggest that the lyase activity plays a major role in regulating these syntheses. To obtain more information on the structure-function relationship we isolated a full-length cDNA clone encoding guinea pig P450c17. Northern blots of total RNA extracted from the testis, ovary, and adrenals of the guinea pig show that the P450c17 cDNA hybridized with a predicted 1.8-kb mRNA and with two other mRNAs of 3 and 4 kb. No signal other than the 1.8-kb mRNA was observed in the human adrenocortical NCI-H295 cells. Activation of the cAMP-dependent protein kinase A pathway increased the levels of the three mRNAs. Transfection of vectors expressing guinea pig P450c17 cDNA into nonsteroidogenic cells confers 17 alpha-hydroxylase and 17,20-lyase activities, showing that a single protein in the guinea pig supports both activities. However, the analysis of the enzymatic properties showed that the guinea pig P450c17 recombinant, in contrast to the human, supports hydroxylase and lyase activities only with delta 4 substrates. These results were further confirmed with isolated guinea pig adrenocortical cells. Our data demonstrate, first, that guinea pig P450c17 cDNA hybridizes with three different transcripts and second, that the expressed protein has characteristics associated exclusively with the guinea pig enzyme.
Animals↗
Acute in vivo effects of ACTH on the expression of steroidogenic acute regulatory protein in rat adrenal.
We have studied the effects of adrenocorticotropin (ACTH) on the expression of steroidogenic acute regulatory protein (StAR) in rat adrenals in vivo. Following ACTH stimulation, the level of StAR mRNA was increased within 1 h in zona glomerulosa (ZG) and zona fasciculata-reticularis (ZFR), with a maximum increase at 3 h. The increase in StAR protein was delayed when compared to its mRNA. The increase in the mitochondrial StAR protein at 3 h was concomitant with that of the homogenate indicating that the entry of StAR into mitochondria might not be necessary to increase steroidogenesis during the early stimulatory phase. In conclusion, we showed that ACTH increases StAR mRNA followed, after a delay, by an increase in the level of StAR protein; this suggests that post-translational modifications of StAR precursor occur during the early stimulatory phase and this occurs before the apparent translation of the newly formed mRNA.
Adrenocorticotropic Hormone↗