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Biomedical subjects

A Florio

Publications and source records attributed to A Florio.

34 records · Page 2Linked to original sources
PubMed1992

A new ELISA assay for islet cell surface antibodies determination in type 1 diabetes mellitus of recent onset.

Sera from 46 newly diagnosed type 1 diabetic patients and from 100 normal controls were screened for ICA Cf ICA (standard immunofluorescence test) and for ICSA (ELISA assay) detection. Among 46 patients, 33 (71.7%) were ICA positive (16 of them also Cf ICA positive) and 21 resulted ICSA positive (45%). No correlation was found between ICSA, ICA and Cf ICA respectively. Therefore the ICSA detection, by a new and sensitive ELISA assay, in addition to ICA immunofluorescence determination, may be of significant diagnostic value in IDDM of recent onset.

Adolescent↗
PubMed1991

Transurethral resection of the prostate and metastatic prostate cancer.

Demonstration of malignant cells in blood specimens collected during transurethral resection of the prostate (TURP) has implicated TURP in the dissemination of prostatic cancer. Of 153 patients who underwent radiation therapy for prostate cancer between January 1977 and June 1990 and were retrospectively analyzed, 93 were evaluable. Fifty-nine patients required TURP before radiation therapy for prostatic obstruction (BPH and/or cancer); the remaining 34 patients underwent radiation therapy after fine-needle aspiration biopsy. No statistically significant difference in failure rate could be detected between these groups, with a failure rate of 47% (28 of 59 patients) at a median follow-up time of 49 months (range, 8 to 146 months) in the TURP group versus a failure rate of 47% (16 of 34 patients) at a median follow-up time of 50 months (range, 3 to 122 months) in the group who underwent biopsy only (Fisher's exact test, P = 0.23). Within the confines of this retrospective study, it appeared that TURP did not enhance the development of metastatic disease.

Adenocarcinoma↗
PubMed1990

[Angioplasty of the deep femoral artery using a semirigid PTFE prosthesis. Experimental studies].

Underlining the importance of Profundus Femoral Artery and its capacity to make up for lower limb's haemodynamics during obliterative arteriopathy, the Authors conceive a funnel-shaped intravascular prosthesis, Goretex constructed (PTFE), to introduce, in case of obstruction, in the artery lumen with a suitable instrument. The research is performed through ten oldster dogs whose average weight is twenty-four kilograms. Isolated the deep Femoral Artery and carried out an arteriotomy of about two centimetres on the Common Femoral Artery, we bring the prothesis in Profundis Femoral Artery and we fix it to the wall of the Common Femoral Artery with 8/O microsurgical dots. After thirty days, the arteriographic test shows the patency of the endoprosthesis with normal peripheral flow in nine animals out of ten. By virtue of this preliminary experiment, the Authors point out the semplicity of this intervention, also feasible in local anaesthesia, the good tolerability of the prosthesis and its perfect integration with the surrounding arterious wall, without shifts or dissecting laminar flows between the external prosthesis wall and the vessel lumen of the animal.

Aged↗
PubMed1988

A kinetic thermodynamic phenomenological approach to genetic expression of heat-shock proteins.

The approach of viewing complex biochemical phenomena as autocatalytic relaxation processes has been introduced previously (Liquori & Tripiciano, 1980; Liquori & Florio, 1985). In the present work this formalism is extended from its original framework regarding cell growth, to the problem of genetic expression. The case of the heat-shock response in organisms ranging from man to bacteria is discussed. Finally, we give some biochemical examples in which the new approach underlines evident temporal co-operativity.

Gene Expression Regulation↗
PubMed1983

Decreased antithrombin III activity in diabetes may be due to non-enzymatic glycosylation--a preliminary report.

Antithrombin III activity was moderately but significantly decreased in insulin dependent diabetics (p less than 0.005), while no difference has been found between the diabetic (n = 25) and control groups (n = 22) in antithrombin III plasma concentration. Moreover, antithrombin III activity was inversely correlated with glycosylated plasma proteins (r = 0.57; p less than 0.01). These data show that in diabetes there is a depressed biological activity of antithrombin III and suggest that this reflects nonenzymatic glycosylation of antithrombin III protein.

Adult↗
PubMed1983

[Lipid-metabolic and blood coagulation factors in chronic peripheral arteriopathies].

The authors have tested serum levels of cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol, with the plasmatic levels of Antithrombin III and other parameters (Quick time, PTT, fibrinogenaemia and platelets) in patients affected by chronic obstructive arterious diseases in various stages including a group of patients affected by diabetes mellitus. The results show that there is a correlation between the behaviour of the parameter tested and the follow-up of the diseases, particularly for HDL and LDL cholesterol and Antithrombin III. The authors suggest the use of these tests for monitoring this kind of vascular peripheral diseases.

Adult↗
PubMed1982

Effect of anaesthesia on serum levels of LH and FSH in man with and without GnRH test.

The effect of anaesthesia with atropine and pentobarbital (PB) in three groups of volunteers has been tested. The dose used for the anaesthesia was 20 mg/kg body weight of PB. The experiment was performed with and without stimulation test with GnRH. Anaesthesia was shown to increase serum levels of LH and to increase the response to stimulation with GnRH. Atropine and mental stress seem to have no effect on the hormones. No variation in serum levels of FSH in the anaesthesized volunteers was noted. The data obtained have been statistically controlled.

Adult↗
PubMed1979

[Effect of pretreatment with metoclopramide on plasma prolactin response to methergoline].

The Authors examined 20 voluntary women without endocrine diseases. The women took 4 mg of metergoline orally and 30-60-90-120-180-240 minutes after the medicament was given the serum prolactin levels were tested. After 3 weeks, 14 among 20 subjects repeated the test assuming during the 3 days before 50 mg of metoclopramide orally once a day. The Authors found a remarkable decline of prolactin serum levels after metergoline administration in all subjects. After metoclopramide administration prolactin serum levels increased meaningly. Metergoline administration gave again considerable fall of prolactin serum levels in the 14 subjects. From the data the Authors affirm that metergoline inhibits prolactin secretion with an antiserotonine action

Adult↗
PubMed1978

[Effect of metoclopramide on growth hormone (GH) release induced with ACTH].

We examined 10 volunteers subjects, sound and exempt from endocrine disease. The rapid injection of 0,25 mg of ACTH (Synacten) was followed by a distinct increase of plasma Growth Hormone (GH) within 30 or 60 min in 9 of 10 normal volunteers. The test were then repeated in the 12 "responders" after previous subministration of Metoclopramide and the GH response was totally abolished.

Adrenocorticotropic Hormone↗
PubMed1978

[Effect of TRH on growth hormone (GH) release induced with ACTH].

We examined 14 volunteers subjects sound and exempt from en docrine disease. The rapid injection of 0,25 mg of ACTH (Synacten) was followed by a distinct increase of plasma Hormone (GH) within 30 or 60 min in 12 of 14 normal volunteers. The test were the 12 "responders after previous subministration of 200 g of TRH and the GH response was totally abolished.

Adolescent↗