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Biomedical subjects

A Fujiwara

Publications and source records attributed to A Fujiwara.

At least 127 records · Page 7Linked to original sources
PubMed1989

Mesonephric duct hyperplasia of the uterus. Report of two cases and three other cases of mesonephric duct remnant with findings of mucin histochemistry and lectin binding immunohistochemistry.

Two cases of mesonephric duct hyperplasia of the uterus, together with three cases of uterine mesonephric duct remnant, are reported. The latter cases were identified by serial transverse sectioning of 42 surgically resected uteri, yielding a frequency of approximately 7%. Histochemical studies showed no mucin in the cytoplasm of cells lining the mesonephric duct remnant, as well as mesonephric duct hyperplasia. In contrast, the endocervical epithelium had abundant neutral mucin, sialomucin and sulfomucin. Immunohistochemical studies on lectin binding showed positive binding of PNA and SBA lectins only to the lining cells of mesonephric duct hyperplasia.

Adult↗
PubMed1988

Sclerosing stromal tumor of the ovary associated with endometrial adenocarcinoma: a case report.

The first case of ovarian sclerosing stromal tumor associated with endometrial adenocarcinoma in a 44-year-old woman is presented. By ultrastructural study, typical steroid-secreting type theca cells and various transitional tumor cells from fibroblast-like cells to theca-like cells were observed in the ovarian tumor. The clinical history of the patient and the ultrastructural study of this tumor suggested hormonal activity, but no apparent evidence of active hormone secretion was detected by plasma analysis for steroid hormones.

Adenocarcinoma↗
PubMed1988

[Detection of human papillomavirus types 16 and 18 DNA sequences in the screening of the cells related with carcinoma in situ of the uterine cervix--application of in situ hybridization for cytologic diagnosis].

In order to investigate the relationship between the presence of human papillomavirus (HPV) DNA and cervical carcinoma, we examined the cervical screening cells as well as the biopsy specimens obtained from 3 cases of severe dysplasia, 13 cases of carcinoma in situ (CIS) and 2 cases of microinvasive carcinoma for the presence of HPV types 6, 11, 16 and 18 DNA by DNA-DNA in situ hybridization using the biotinylated HPV DNA probes. The results of in situ hybridization analysis revealed that HPV 16 DNA sequences were detected in the nuclei of koilocytosis of severe dysplasia and CIS cases. The nuclei of atypical cells obtained from cervical screening cells were positive for HPV 16 or 18 DNA sequences. Two CIS cases were positive for the presence of HPV 16 and 18 DNA sequences. None of them contained HPV 6/11 DNA sequences. Eighteen cervical screening cases were examined and 10 contained HPV 16 DNA sequences and 6 contained HPV 18 DNA sequences. We suggest that the identification of HPV DNA types in cervical screening cells by in situ hybridization might be of diagnostic and prognostic value in early cervical neoplasia.

Carcinoma in Situ↗
PubMed1988

[Synchronous double cancers in the ovary and kidney--a case report].

UNLABELLED: A first case of synchronous double cancers in the ovary and kidney is presented. The patient is a 37-year-old woman complaining of an abdominal mass. Histopathological findings showed a mucinous cystadenocarcinoma of the right ovary and a renal cell carcinoma, common type, clear cell subtype of the right kidney. A metastatic renal cell carcinoma was found on the pelvic peritoneum and characteristic renal cancer cells were identified in the ascitic fluid. ( EVALUATIONS: ovarian cancer, stage Ia; renal cancer, stage IV) The patient received postoperative chemotherapy and, after recovery, was discharged in a healthy condition. Her 11-month postoperative evaluation revealed no evidence of disease.

Adenocarcinoma, Mucinous↗
PubMed1988

[Clinical study of cefuzonam in the field of obstetrics and gynecology].

Cefuzonam (CZON, L-105) was used clinically for the treatment of obstetrical and gynecological infections at a dosage of 1 g once or twice daily by intravenous drip infusion. The results obtained are summarized as follows. 1. Clinical effects of CZON were analyzed in 10 patients, including 5 patients with intrapelvic infections, 3 with intrauterine infections, and 1 each with adnexitis and an external genital infection. Excellent responses were observed in 1 patient (11.1%), good responses in 7 (77.8%), poor responses in 1 (11.1%), and one remaining case was unevaluable. The efficacy ratio was 88.9%. 2. Upon the treatment, eradications of causative bacteria were observed in all 4 cases tested. Staphylococcus sp. (2 strains), Staphylococcus epidermidis (1 strain), Klebsiella oxytoca (1 strain) and Pseudomonas putida (1 strain) were all eradicated by the CZON treatment. 3. The safety of the drug was analyzed in the 10 patients and rash occurred in 1 patient as a side effect. 4. One patient showed eosinophilia after the CZON treatment. It appeared that CZON would be useful for the treatment of obstetrical and gynecological infections.

Adult↗
PubMed1988

Evidence for modulation by ATP of amino acid transport in human placenta.

The effect of ATP on placental amino acid transport was studied by measuring the uptake of alpha-(methylamino)-isobutyrate in brush border microvillous plasma membrane vesicles prepared from human full-term placental syncytiotrophoblasts which were incubated with or without ATP. The presence of a Na+ gradient from the outside to the inside of the vesicles prepared after incubation with ATP resulted in a higher initial rate and an increased transport of alpha-(methylamino)-isobutyrate, while Na+ gradient-independent alpha-(methylamino)-isobutyrate uptake was not different in either type of membrane vesicle. The increase in transport activity was not inhibited by cycloheximide. Kinetic analysis showed that ATP enhanced transport activity by increasing the maximal velocity (Vmax) of transport, without significant changes in the affinity (Km) of the carrier for the substrate, suggesting an increase in carrier number in placental syncytiotrophoblasts incubated with ATP.

Adenosine Diphosphate↗
PubMed1987

Characterization of amino acid transport systems in human placental brush-border membrane vesicles.

Brush-border microvillous plasma membrane vesicles were prepared from human full-term placental syncytiotrophoblasts and purified 33-fold from the homogenate with reference to a membrane marker enzyme, alkaline phosphatase (EC 3.1.3.1). Transport of alpha-(methylamino)isobutyrate by the membrane vesicles was stimulated in the presence of an Na+ gradient from the outside to the inside of the vesicles. The initial rate of uptake in a 10-s period was enhanced with increasing concentration of Na+ in the external medium. The level of alpha-(methylamino)isobutyrate transported into the vesicles reached a maximum 1 min after the start of incubation at 37 degrees C, and then decreased with time due to efflux. Extrapolation to infinite medium osmolarity showed no uptake, indicating transport of alpha-(methylamino)isobutyrate into membrane vesicles. The initial rate of uptake was dependent on temperature and pH: the highest rate occurred at 37 degrees C and the optimal pH was 8.0. When the alpha-(methylamino)isobutyrate concentration was varied, the initial rate of uptake dependent on an Na+ gradient (out greater than in) obeyed Michaelis-Menten kinetics with Km and Vmax values of 1.07 mM and 3.23 nmol/10 s per mg of protein, respectively. Cross-inhibition patterns indicated that at least three Na+-dependent and two Na+-independent carrier-mediated pathways existed in the human placental brush border. One Na+-dependent pathway interacted with all substrates tested. Another Na+-dependent route interacted with L-proline, alpha-(methylamino)isobutyrate, and L-methionine, while a third pathway was selective for L-methionine. One Na+-independent pathway was selective for L-cysteine, while the other pathway interacted with all substrates tested.

Amino Acids↗
PubMed1987

[A case report of uterine carcinosarcoma with alpha-fetoprotein (AFP) production].

A case of carcinosarcoma, mixture of endometrial stromal sarcoma and adenocarcinoma, of the uterus with alpha-fetoprotein (AFP) production in a 69-year-old woman was reported. By the results of serum AFP levels (preoperative: 2,950.0 ng/ml, post-operative and chemotherapy: 2.1 ng/ml) and immunohistochemical localization of AFP, the AFP was thought to be produced from this tumor. Immunoreactive AFP was found in the carcinomatous components and no AFP was present in the sarcomatous components at all. It seems from this that immunohistochemical study supports the composition theory of mixed mullerian sarcoma of the uterus.

Aged↗
PubMed1987

[Sister chromatid exchange (SCE) in lymphocytes of patients with cancer of the cervix uteri].

The frequency of spontaneous and Mitomycin C (MMC)-induced sister chromatid exchange (SCE) was investigated in peripheral lymphocytes of 31 untreated patients with cervical cancer (stage 0:7 cases, stage I:9 cases, stage II:6 cases and stage III:9 cases), as well as of 7 untreated patients with myoma uteri and 16 healthy women. Factors affecting SCE frequency were also investigated. Age, smoking and atomic bomb exposure did not influence SCE frequency. However, a family history of cancer significantly increased MMC-induced SCE frequency in the cancer group. The mean spontaneous SCE frequency in the cancer group (9.83 +/- 1.74) was significantly higher than that in the myoma group (8.13 +/- 0.81) and healthy women (7.59 +/- 0.85). The mitomycin C-induced SCE frequency was also higher in the cancer group. Spontaneous and MMC-induced SCE frequency gradually increased with the progression of cervical cancer. All chromosome groups equally contributed to the increased SCE frequency in the cancer group. These results suggest that deoxyribonucleic acid of all chromosomes is damaged in patients with cervical cancer.

Adult↗
PubMed1987

[A study of gastric cancer invasion of the duodenum].

Gastric cancers that have invaded in the duodenum were clinicopathologically studied in 83 patients in an attempt to clarify surgical therapeutic problems. The classification and results are itemized below. 1) The extensive forms of these cancers were found to include the types of continual superficial extension (12 patients), expansion (11), deep-layer (39), whole-layer (9), non-continual extensive intralymphatic duct type (8) and specific type (4). 2) More patients manifesting a deep-layer or intralymphatic duct cancers were Borr. 3 and 4 types with a longer distance of infiltration. All of the 14 patients with an aw (+) showed these patterns of extension. 3) The n3 (+) was more common with Nos. 12, 13 and 14 lymph nodes, and similar results were seen by RI lymphography. Thus, special care may have to be exercised in treating the duodenal stump in the gastric cancers that have invaded the pyloric ring. Pancreato-duodenectomy may be required for the removal of lymph nodes in patients with gastric cancers that show a severe infiltration into the duodenum.

Duodenal Neoplasms↗