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Biomedical subjects

A G Leary

Publications and source records attributed to A G Leary.

27 records · Page 2Linked to original sources
PubMed1977

Cell-mediated immune responses to Pseudomonas aeruginosa.

Of six healthy laboratory workers with no history of illness caused by Pseudomonas aeruginosa, four exhibited positive skin responses at 24 hours, strong inhibition of leukocyte migration, and marked stimulation of blastogenesis of peripheral lymphocytes when exposed to a "cocktail" of three phenol-killed strains of P aeruginosa. There was no correlation between the extent of these responses with serum hemagglutinin titers. It is postulated that cell-mediated immunity against P aeruginosa may have a biologic role in host defense.

Antigens, Bacterial↗
PubMed1977

The effect of antibiotics on cell-mediated immunity.

Normal human lymphocytes in culture were stimulated by the addition of phytohemagglutinin, and deoxyribonucleic acid (DNA) synthesis was measured by the incorporation of tritiated thymidine. The effect of 11 commonly used antibiotics on DNA synthesis then was measured by adding each antibiotic to the culture in concentrations ranging from 2 to 64 microng/ml, a range which covers the plasma levels obtained during customary clinical therapy. Severe dose-dependent suppression of DNA synthesis was found in the presence of two preparations of minocycline, oxytetracycline, and the ascorbic acid salt of tetracycline. Less severe but stil significant suppression was found in the presence of chloramphenicol, clindamycin, tetracycline, and ascorbic acid alone. No effect was noted when penicillin, carbenicillin, or cephalothin was added, and slight stimulation was found in the presence of gentamicin. It is postulated that these findings may help to explain fungal and viral superinfection following antibiotic therapy and that they may play a role in the failure of antibiotic therapy to eliminate some infections.

Anti-Bacterial Agents↗
PubMed1976

use of F(ab')2 fragment in experimental skin transplantation for burns.

In a series of experiments involving approximately 500 animals, burned rats were administered the F(ab')2 fragment of antidonor IgG to study the survival of incompatible skin allografts. No prolongation was noted when treatment consisted of antidonor F(ab')2 fragment alone. This was true whether the fragment was raised against donor spleen cells of donor skin, whether the skin allograft was partial or full thickness and whether the systemic treatment of the recipients was preceded by a period of incubation of the graft in F(ab')2 fragment or not. Significant prolongation of survival was noted when the fragment was raised against both donor and recipient histocompatibility antigen and when the donor skin was incubated in steroids as well as in F(ab')2 fragment prior to grafting. Additional findings included the confirmation of prolonged allograft survival by increasing burn size and the prolongation of allograft survival by the presence of intact eschar.

Animals↗
PubMed1975

Cell-mediated immunity in the uremic rat.

The popliteal node assay of the graft-versus-host reaction in the inbred rat provides a sensitive measure of cell-mediated immunity. Spleen cells from uremic Lewis rats as well as normal cells cultured in uremic serum demonstrated significant immunosuppression which was proportional to the severity of the uremia.

Animals↗