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Biomedical subjects

A G Volchek

Publications and source records attributed to A G Volchek.

31 records · Page 2Linked to original sources
PubMedDate not supplied

[Progestin and estrogen concentrations during the reproduction of sables].

Competitive protein-binding analysis and radioimmune method was applied to the study of the content of progestins and estrogens in the blood and urine in female sables of different age (kept in cages) at periods corresponding to the estrus, diapause and true pregnancy. Sables with a normal course of pregnancy displayed a regular increase in the progestin level already at the period of diapause, although relatively small in value. The period of true pregnancy is attended by a pronounced elevation of the progestine level, with a maximum in the middle of pregnancy. The estrogen level falls at the period of diapause (as compared with the estrus period). The period of true pregnancy is accompanied by a marked elevation of the estrogen level several days before labour, with a fall after them. Examination of the estrogen and progestin levels in sables can aid in the establishment of pregnancy and differentiation of its normal course from the pathological one.

Age Factors↗
PubMedDate not supplied

[Experience in the use of competitive binding of hormones with proteins for the determination of non-metabolized glucocorticoids in the blood and urine in some pituitary-adrenal diseases].

A method of competitive protein-binding analysis was applied to the study of nonmetabolized glucocorticoids (NMG) in the plasma and urine of healthy persons and those suffering from hyper- and hypocorticism. NMG determination by this method reflected adequately the funcitonal condition of the adrenal cortex under normal conditions and in hypophyseo-adrenal diseases, including tests with ACTH and dexametazan, and coordinated with the results obtained by standard chemical methods.

Adrenal Insufficiency↗
PubMedDate not supplied

[Determination of human blood and urine progestins by the method of competitive protein binding on the basis of guinea pig transprogestin].

The use as a testing system of transprogestin or of progesterone-binding protein (obtained from a pregnant guinea pig) with a high specificity to progesterone and a number of its affiliated metabolites increased the sensitivity of the method considerably, permitting to determine progestins in the blood and urine of nonpregnant women (at various phases of the sexual cycle) and in men. Dynamics of the given progestins in the urine correlates with the changes of their blood level and permits complete and objective assessment of the ovarian and placental functions in women with various physiological conditions.

Adult↗
PubMedDate not supplied

[Rabbit testosterone-binding globulin and its use in concurrent protein-binding analysis].

The existence of testosterone-binding globulin (TBC) homogeneous by the association constant (of the 10(8) M-1 order) and sedimentation characteristics (sedimentation coefficient--4S) was demonstrated in various groups of rabbits (mature males and females, pregnant females and fetuses, immature males). The level of the TBG binding capacity failed to show any marked differences in all the ontogenetic groups. The TBG level was rather close in the rabbit mother and fetus. Estradiol, estron, progesterone, cortizol possessed no affinity to this protein. Dehydrotestosterone affinity was about three times greater than that of testosterone. When TBG was used in the competitive protein-binding analysis of testosterone and other protein-bound androgens in the blood plasma of men, women and rabbits (male and female) there were obtained results coinciding with those of other authors. This permitted to recommend TBG for wide practical use in the competitive protein-binding analysis.

Androgens↗
PubMedDate not supplied

[Study of the glucocorticoid receptors of the cytosol in various organs of the rat].

A method of hard phasic separation of free and bound forms of the hormone demonstrated the presence of specific (saturated) and nonspecific (unsaturated) binding of dexamethazone in the cytosol of various rat organs; and increase of the sorbent concentration promoted a better detection of the first type of binding on account of selective depression of binding of the second type. Cortisol was bound with dexamethazone receptors of various rat organs in the binding sites common with dexamethazone, replacing the latter from its complex with the receptors. The constant of dexamethazone association with its receptors in the brain, liver lungs, spleen, thymus, and kidney cytosol was of the order of 10(8) M-1, with the binding capacity of cytosol of these organs within the range of from 0.6 to 4 ng hormone per 1 g of raw weight.

Animals↗