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Biomedical subjects

A G Williams

Publications and source records attributed to A G Williams.

At least 19 recordsLinked to original sources
PubMed2006

Strange electric form factor of the proton.

By combining the constraints of charge symmetry with new chiral extrapolation techniques and recent low-mass quenched lattice QCD simulations of the individual quark contributions to the electric charge radii of the baryon octet, we obtain an accurate determination of the strange electric charge radius of the proton. While this analysis provides a value for G(E)(s)(Q(2) = 0.1 GeV(2)) in agreement with the best current data, the theoretical error is comparable with that expected from future HAPPEX results from JLab. Together with the earlier determination of G(M)(s), this result considerably constrains the role of hidden flavor in the structure of the nucleon.

Journal Article↗
PubMed2006

Can simple anthropometric and physical performance tests track training-induced changes in maximal box-lifting ability?

The objective of this research was to determine if tests of lifting strength and anthropometry could be used to assess training-induced changes in box-lift performance. Ninety-two British Army recruits completed a 10-week training programme. Isometric and dynamic lifting strength, body composition and maximal box-lift to 1.45 m and 1.70 m were assessed before and after training. Training significantly increased box-lift performance, dynamic lifting strength and fat-free mass, and reduced body fat. Statistically significant regression models (adjusted R(2) of 0.299 and 0.152) were produced that predicted the changes in box-lift performance from changes in body composition and lifting strength. However, the 95% limits of agreement between the observed and predicted change in box-lift performance showed wide margins of error (11-13 kg; 26-29%). Training-induced changes in box-lift performance appear to be highly specific, which suggests that both training and testing procedures should also be specific to box-lift performance.

Adult↗
PubMed2006

Pulmonary O2 uptake on-kinetics in endurance- and sprint-trained master athletes.

The purpose of this study was to characterise the VO2 kinetic response to moderate intensity cycle exercise in endurance-trained (END) and sprint or power-trained (SPR) track and field master athletes ranging in age from 45 to 85 years. We hypothesised that the time constant (tau) describing the Phase II VO2 on-response would be smaller in the END compared to the SPR athletes, and that the tau would become greater with increasing age in both groups. Eighty-four master athletes who were competing at either the British or European Veteran Athletics Championships acted as subjects, and were classified as either END (800 m - marathon; n = 41), or SPR (100 - 400 m and field events; n = 43) specialists. Subjects completed two 6 minute "step" transitions to a work rate of moderate intensity on a cycle ergometer and pulmonary gas exchange was measured breath-by-breath. Analysis of variance revealed that SPR athletes had slower VO2 on-kinetics (i.e., greater tau) compared to END athletes at each of the age groups studied: 46 - 55 yrs (END: 25 +/- 6 vs. SPR: 36 +/- 9 s; p < 0.10), 56 - 65 yrs (END: 25 +/- 5 vs. SPR: 35 +/- 10 s; p < 0.05), 66 - 75 yrs (END: 29 +/- 10 vs. SPR: 40 +/- 13 s; p < 0.05), and 76 - 85 yrs (END: 31 +/- 10 vs. SPR: 51 +/- 18 s; p < 0.05). The VO2 on-kinetics became slower with advancing age in the SPR athletes (p < 0.05 between 56 - 65 and 76 - 85 yrs) but were not significantly changed in the END athletes. The slower VO2 on-kinetics in SPR compared to END master athletes is consistent both with differences in physiology (e.g., muscle fibre type, oxidative/glycolytic capacity) and training between these specialist athletes. Master END athletes have similar tau values to their younger counterparts (approximately 25 s) suggesting that participation in endurance exercise training limits the slowing of VO2 on-kinetics with age in this population.

Aged↗
PubMed2006

Glutamate dehydrogenase activity in lactobacilli and the use of glutamate dehydrogenase-producing adjunct Lactobacillus spp. cultures in the manufacture of cheddar cheese.

AIMS: The study was undertaken to investigate the occurrence of glutamate dehydrogenase activity in different species of lactobacilli, and to determine, in a series of cheese-making trials, the effects of glutamate dehydrogenase-producing adjunct cultures on sensory attribute development during the maturation of cheddar cheese. METHODS AND RESULTS: The presence of dehydrogenase activity with glutamate as substrate was monitored in cell lysates of >100 strains from 30 different species of lactobacilli using a qualitative colorimetric plate screening assay. Activity was detectable in 25 of the 29 representative species obtained from culture collections and in 12 of the 13 non-starter species isolated from cheese. There were pronounced interspecies and strain differences in the occurrence, level and pyridine nucleotide specificity of the glutamate dehydrogenase activity detected. Among the non-starter lactobacilli the highest frequency of enzyme occurrence and activity was detected in the Lactobacillus plantarum isolates. The establishment of glutamate dehydrogenase-producing adjunct strains in the predominant population of lactobacilli in the cheese curd affected the formation of a number of volatile compounds in ripening cheddar cheese, while the presence of Lact. plantarum strains, in particular, was associated with an intensification and acceleration of aroma and flavour development during the maturation period. CONCLUSIONS: Glutamate dehydrogenase formation by lactobacilli is a strain-dependent metabolic attribute, and adjunct cultures expressing the activity that are able to proliferate during cheese ripening have a positive impact on the rate of development and the intensity of cheddar cheese aroma and flavour development. SIGNIFICANCE AND IMPACT OF THE STUDY: It has been demonstrated that some strains of glutamate dehydrogenase-producing lactobacilli have potential use as adjunct cultures to accelerate and intensify aroma and flavour formation during the manufacture of cheddar and, by analogy, other similar varieties of cheese. The importance of phenotypic discriminative monitoring of the dominant lactobacilli present during ripening to confirm adjunct establishment and population complexity was highlighted as was the requirement to establish the metabolic attributes of the non-starter population in uninoculated control cheeses in comparative trials.

Cheese↗
PubMed2005

Precise determination of the strangeness magnetic moment of the nucleon.

By combining the constraints of charge symmetry with new chiral extrapolation techniques and recent low mass quenched lattice-QCD simulations of the individual quark contributions to the magnetic moments of the nucleon octet, we obtain a precise determination of the strange magnetic moment of the proton. The result, namely, G(s)(M)=(-0.046 +/- 0.019)mu(N) is consistent with the latest experimental measurements but an order of magnitude more precise. This poses a tremendous challenge for future experiments.

Journal Article↗
PubMed2004

The acute effects of exercise and glucose ingestion on circulating angiotensin-converting enzyme in humans.

Angiotensin-converting enzyme (ACE) activity has been suggested as a determinant of some exercise phenotypes via some studies that have associated the ACE gene with exercise performance, although several studies provide conflicting evidence regarding the influence of the ACE gene. The relationships between ACE phenotype (ACE activity) and various exercise parameters should also be examined. An early step in this process is to determine whether common environmental stimuli such as exercise and diet have acute effects on ACE activity. In this study, the acute effects of aerobic exercise, resistance exercise and glucose ingestion on circulating ACE activity were examined. On three separate occasions, 20 healthy adult volunteers (9 female and 11 male) performed 20 min of submaximal cycle exercise at 70-80% of maximal heart rate, four sets of ten repetitions of unilateral leg extension resistance exercise at ten-repetition maximum load, or ingested 1 g kg(-1) glucose. Circulating ACE activity was assessed for 1 h after each intervention using a modified fluorometric method. Pre-intervention ACE activity remained remarkably stable across test days (difference < or =1.8%). Furthermore, there was no significant change in circulating ACE activity following any of the interventions (difference from pre-intervention values < or =6.8% when unadjusted for plasma volume changes, < or =4.5% when adjusted for plasma volume changes). These results suggest that acute exercise and glucose ingestion interventions as used here do not affect circulating ACE activity. These findings are an early step in illuminating the relationships between ACE activity and various exercise parameters.

Adult↗
PubMed2004

The effect of alpha-ketoglutaric acid on amino acid utilization by nonstarter Lactobacillus spp. isolated from Cheddar cheese.

AIMS: To examine the effect of alpha-ketoglutaric acid (alpha-KG) on the utilization and catabolism of amino acids by strains of nonstarter lactobacilli isolated from Cheddar cheese. METHODS AND RESULTS: The effect of alpha-KG in the growth medium of nonstarter lactobacilli on amino acid metabolism, catabolite levels, peptide hydrolase and aminotransferase activities was examined. The pattern of amino acid utilization, catabolite formation and aminotransferase activity was affected by keto acid. CONCLUSIONS: Amino acid conversion into cheese aroma and flavour compounds by nonstarter lactobacilli is enhanced in the presence of alpha-ketoglutarate. SIGNIFICANCE AND IMPACT OF THE STUDY: Increasing the availability of alpha-ketoglutarate in cheese offers a possible method of reducing the maturation period by accelerating the rate of character compound formation from amino acids by the nonstarter lactobacilli.

Amino Acids↗
PubMed2003

Genotypic and phenotypic characterization of the dynamics of the lactic acid bacterial population of adjunct-containing Cheddar cheese manufactured from raw and microfiltered pasteurised milk.

AIMS: This study investigates the dynamics of the microflora, particularly the lactobacilli, in Cheddar cheese manufactured from raw and microfiltered milk containing different adjunct cultures. METHODS AND RESULTS: Sixteen cheeses - raw milk, adjunct and control cheeses - were manufactured in four trials. Lactobacilli were identified by PCR methods in one trial, and by phenotypic typing for all trials. Numbers of lactobacilli were significantly different at day 1 and 3 months in the control and adjunct-containing cheeses. In the raw milk cheeses, Lactobacillus paracasei was detected throughout ripening, Lact. curvatus at the end, and Lact. plantarum at day 1 only. Lactobacillus strain diversity decreased from raw, control to adjunct cheeses. Enteroccoci and coliform numbers further differentiated raw cheeses from the others. Lactococcal starter numbers also differed in the three cheese types and differences were observed within adjunct cheeses. Although adjunct lactobacilli dominated in the cheese to which they were added, strains with similar phenotypic profiles were also detected on occasions in some of the control cheeses. CONCLUSIONS: The addition of adjunct lactobacilli modified the growth kinetics of both adventitious lactobacilli and starter lactococci during ripening. Appropriate strain tracking is necessary to monitor changes in the population profiles of control and experimental cheeses in trials utilizing adjunct cultures. SIGNIFICANCE AND IMPACT OF THE STUDY: Investigations of the role of adjunct strain(s) in cheeses may be complicated by the interactions between the adjunct and the other cheese strains, and effective strain monitoring by genotypic or phenotypic methods is essential if valid comparisons are to be made.

Animals↗
PubMed2002

Resistance training and the enhancement of the gains in material-handling ability and physical fitness of British Army recruits during basic training.

The aim was to evaluate the efficacy of a modified British Army basic training that included resistance training in improving material-handling performance and physical fitness, and to compare the modified training directly with the normal basic training. Forty-three males [19.2 (2.6) years of age, 1764 (72) mm in height, 73.0 (10.6) kg in mass] and nine females [19.1 (2.2) years, 1641 (67) mm, 62.0 (7.2) kg] performed the modified basic training. Testing occurred in the week before and in the final week of the 11-week basic training. Improvements with the modified training were observed for all six material-handling tests, including 8-12% for maximal box lifting, 15-19% for repetitive lifting and carrying and 9-17% for loaded marching (all p < 0.01), and other established measures of aerobic fitness, strength and kinanthropometric characteristics. Significantly greater improvements were observed for the modified training compared with the normal training in maximal box lift to 1.45 m (12.4 versus 1.7%, p < 0.01), 3.2 km loaded march performance with 15 kg (8.9 versus 3.6%, p < 0.05), estimated fat-free mass (4.2 versus 1.5%, p < 0.001), predicted VO(2max) (1 min(-1)) (9.3 versus 4.1%, p < 0.01) and dynamic lift to 1.45 m (15.5 versus 0.2%, p < 0.001). It was concluded that the improvements in material-handling ability and other aspects of physical fitness brought about in recruits by British Army basic training can be enhanced by the use of a physical training programme that includes a carefully designed resistance training element. Of particular note are the improvements shown in performance on material-handling tasks that require muscular strength, as these represent many of the tasks that soldiers encounter in their military careers.

Adolescent↗
PubMed2002

Endurance enhancement related to the human angiotensin I-converting enzyme I-D polymorphism is not due to differences in the cardiorespiratory response to training.

Human physical performance is strongly influenced by genetic factors. We have previously reported that the I variant of the human angiotensin I-converting enzyme (ACE) gene is associated with greater endurance performance in mountaineers and Olympic runners and improved performance in army recruits. In this study we examined whether this effect is mediated by improvements in cardiovascular fitness with training in 58 army recruits homozygous for the insertion (I, ACE genotype II) or deletion (D, ACE genotype DD) allele. A submaximal and maximal exercise protocol was used to calculate both the heart rate/oxygen uptake (VO2) relationship and changes in maximal oxygen uptake (VO2max), respectively. There was no significant intergroup difference in VO2max at baseline (P=0.19) or after training (P=0.22). There was no difference between genotypes with training in the heart rate/VO2 elevation (P = 0.79 for the mean difference in mean adjusted heart rates). However, VO2 at all exercise intensities in the submaximal test was lower for all subjects after training and at 80 W the reduction in VO2 was greater for the II subjects compared to DD subjects [mean(SEM)] [1.6 (0.27) and 0.68 (0.27) ml kg(-1) min(-1), respectively; P = 0.02 for mean difference]. The I/D polymorphism may play a role in enhanced endurance performance but this is not mediated by differences in VO2max or the heart rate/VO2 relationship in response to training.

Exercise↗
PubMed2002

Reduction of ammonia and odour emissions from pig slurry under slats using oil and foam layers.

In Europe, new legislation on environmental protection requires reductions in emissions of ammonia and odour from animal production. Animal housing is identified as one of the biggest ammonia emission sources in pig production, but abatement techniques for animal houses often require high initial cost. The aims of this study were to investigate the use of layers of oil and foam on slurry under slats. It was found that relatively low-cost, used frying oil (mainly rapeseed oil) could reduce ammonia and odour emissions by about 50% in weaner accommodation. The cost for this technique was Pound Sterling 2.60 per pig place per year. Foam showed poor ammonia abatement potential. The oil was biodegradable, so increasing methane emissions from stored pig slurry by about 60%.

Ammonia↗
PubMed2001

Kinetics of Cr(VI) reduction by carbonate green rust.

The kinetics of Cr(VI) reduction to Cr(III) by carbonate green rust were studied for a range of reactant concentrations and pH values. Carbonate green rust, [FeII4FeIII2(OH)12][4H2O x CO3], was synthesized by induced hydrolysis (i.e., coprecipitation) of an Fe(ll)/Fe(III) solution held at a constant pH of 8. An average specific surface area of 47 +/- 7 m2 g(-1) was measured for five separate batches of freeze-dried green rust precipitate. Heterogeneous reduction by Fe(II) associated with the carbonate green rust appears to be the dominant pathway controlling Cr(VI) loss from solution. The apparent stoichiometry of the reaction between ferrous iron associated with green rust ([Fe(II)GR]) and Cr(VI) was slightly higherthan the expected 3:1 ratio, possibly due to the presence of other oxidants, such as oxygen, protons, or interlayer carbonate ions. The rate of Cr(VI) reduction was proportional to the green rust surface area concentration, and psuedo-first-order rate coefficients (kobs) ranging from 1.2 x 10(-3) to 11.2 x 10(-3) s(-1) were determined. The effect of pH was small with a 5-fold decrease in rate with increasing pH (from 5.0 to 9.0). At low Cr(VI) concentrations (<200 microM), the rate of reaction was first order with respect to Cr(VI) concentration, whereas, at high Cr(VI) concentrations, rates appearto deviate from first-order kinetics and approach a constant value. Estimated amounts of surface Fe(II) and total Fe(II) suggest that the deviation from first-order kinetics observed at higher Cr(VI) concentrations and the 50-fold decrease in rate observed upon three sequential exposures to Cr(VI) is due to exhaustion of available Fe(II).

Carbonates↗
PubMed2001

Membrane inlet mass spectrometric measurement of O2 and CO2 gradients in cultures of Lactobacillus paracasei and a developing Cheddar cheese ecosystem.

Membrane inlet mass spectrometry was used to measure O2 and CO2 as depth profiles in stab cultures of 0.1% agar Man Rogosa Sharpe medium inoculated with Lactobacillus paracasei CI3. Diffusion of CO2 from the central column of growth into the medium was observed to show lower concentrations where bacteria were absent. CO2 profiles developed in a manner similar to those in Cheddar cheese and O2 was undetectable at similar depths. Gases were analysed in Cheddar cheese over a maturation period of 200 d. O2 was detectable to depths of 13, 6 and 2.5 mm on days 2, 9 and 15, respectively, but then became undetectable at depths of 2.5-3 mm. CO2 concentrations measured within the cheese increased 10-fold from day 2 to day 200 to reach a value of around 15 mM. The progress of measured CO2 concentration over time at a given depth in cheese shows a hyperbolic type increase. Coefficient of regression values increase with depth to a maximum value of R2 = 0.93. In both systems, reductions and increases in CO2 were due to the absence or presence of bacterial growth, respectively. Confocal scanning laser and scanning electron microscopy was used to show spatial heterogeneity of microcolonies within the cheese ecosystem. This information can potentially be used as a non-sensory evaluation of cheese maturity status. Measurement of gases in a cheese ecosystem provides the first description of mass spectrometry being used to monitor the processes of microbial gaseous exchange with respect to O2 and CO2 in a cheese ecosystem.

Carbon Dioxide↗
PubMed2001

Is glucose/amino acid supplementation after exercise an aid to strength training?

BACKGROUND: The precise timing of carbohydrate and amino acid ingestion relative to a bout of resistance exercise may modulate the training effect of the resistance exercise. OBJECTIVE: To assess whether regular glucose/amino acid supplementation immediately after resistance exercise could enhance the gain in muscle strength brought about by resistance training. METHODS: Seven untrained participants with a median age of 23 years and mean (SD) body mass 68.9 (13.5) kg resistance trained on a leg extension machine for five days a week for 10 weeks, using four sets of 10 repetitions. Alternate legs were trained on successive days, one leg each day. Subjects ingested either a supplement including 0.8 g glucose/kg and 0.2 g amino acids/kg, or placebo, on alternate training days immediately after training. Therefore the supplement was always ingested after training the same leg (supplement leg). Isometric, isokinetic, and 1 repetition maximum (RM) strength were measured before, during, and after training. Blood samples were analysed to determine the acute responses of insulin and glucose to resistance exercise and supplementation or placebo. RESULTS: Serum insulin concentration peaked 20 minutes after supplement ingestion at ninefold the placebo level, and remained significantly elevated for at least 80 minutes (p<0.01). Isometric, isokinetic, and 1 RM strength improved on both supplement and placebo legs (p<0.05). There were no significant differences in the gain in strength between the supplement leg and the placebo leg (p>0.05). CONCLUSION: Regular glucose/amino acid supplementation immediately after resistance exercise is unlikely to enhance the gain in muscle strength brought about by resistance training.

Adult↗
PubMed2000

Improving services to bereaved relatives in the emergency department: making healthcare more human.

Death and bereavement are often poorly dealt with in emergency departments. Guidelines exist for optimal care of bereaved relatives. Establishing a limited bereavement program in a busy emergency department is quite feasible. Bereaved relatives appreciate a more "human" approach from hospital staff. Ultimately hospital staff also benefit from confronting issues surrounding death in the emergency department.

Bereavement↗
PubMed2000

The effect on hospital admission profiles of establishing an emergency department observation ward.

OBJECTIVE: To determine the effect of establishing an emergency department observation ward (OW) on admission numbers, average length of stay (ALOS) for the entire hospital and overall bed days for conditions commonly treated in the OW. SETTING: Sir Charles Gairdner Hospital (SCGH), Perth, a tertiary referral teaching hospital. DESIGN: Retrospective analysis of routinely collected hospital data for the 10 most common diagnosis-related group (DRG) categories of patients discharged from the OW for the financial years 1995-96 to 1998-99. Comparison of these data with those for adult patients at the other Perth teaching hospitals over the same period. MAIN OUTCOME MEASURES: For patients in the 10 most common DRGs: numbers of admissions to the OW compared with other inpatient wards; total number of patients admitted to the hospital compared with total bed days; ALOS at SCGH compared with other Perth teaching hospitals. RESULTS: Increased admissions to the OW were paralleled by a decrease in admissions for the same DRG codes to other inpatient wards. ALOS remained approximately the same from 1995-96 to 1998-99 for patients in the OW (one day) and other inpatient wards (4.38 to 4.20 days). However, overall ALOS for patients in these DRGs fell by over a third (from 3.97 to 2.59 days) over this time. The total number of patients in these DRGs treated by the hospital increased by 19% over the four years, but the total number of bed days fell by 23%. By contrast, the ALOS for patients in the same DRGs treated at the other Perth teaching hospitals rose 8% (from 2.12 to 2.28 days). CONCLUSION: Establishment of a formal emergency department OW results in the more efficient management of certain groups of patients, with a decrease in overall hospital bed days and length of stay.

Adolescent↗
PubMed2000

Amino acid fermentation in non-starter Lactobacillus spp. isolated from cheddar cheese.

Amino acid fermentation profiles of nine strains of Lactobacillus spp., initially isolated from a 3-year-old Cheddar cheese, were determined using the Biolog MT microplatetrade mark method. Eight of the isolates were able to ferment amino acids, but only when incubated in the presence of exogenously supplied alpha-ketoglutaric acid that served as an acceptor in the initial transamination step in the fermentative degradation. The range of amino acids catabolized was strain dependent. Amino acid catabolites were detected by gas chromatography mass spectrometry (GCMS) in culture supernatant fluids of a representative non-starter lactic acid bacteria isolate Lactobacillus paracasei CI6.

Amino Acids↗