Biomedical subjects

A Gamundí

Publications and source records attributed to A Gamundí.

5 recordsLinked to original sources

PubMedDate not supplied

Simultaneous determination of vanillylmandelic, homovanillic and 5-hydroxyindoleacetic acids in human urine by thin layer chromatography.

A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with beta-(4-hydroxy-3-phenyl) acetic acid as the internal standard.

Chromatography, Thin Layer↗

PubMedDate not supplied

A simple thin-layer chromatographic method for the analysis of buprenorphine in urine.

A simple and sensitive (at the ng/mL level) method for buprenorphine assay in urine is described. Buprenorphine is extracted by a C18 cartridge, derivatized with dansyl chloride, resolved by a uni-dimensional, two developing mobile phase high-performance thin layer chromatography system and quantified by fluorescence densitometry at 340 nm.

Analgesics, Opioid↗

PubMedDate not supplied

A simple method for plasma cannabinoid separation and quantification.

A sensitive (up to nanogram level) method for resolving a cannabinoid mixture in plasma is described. Cannabinoids were extracted with a C-18 Sep Pak cartridge and derivatized with dansylchloride. Then the derivatives were resolved on thin layer HPTLC silica plates which were developed and quantified by fluorescence densitometry at 340 nm.

Cannabinoids↗

PubMedDate not supplied

Thin-layer chromatographic determination of brain catecholamines and 5-hydroxytryptamine.

A simple and sensitive (up to nanogram level) method to determine norepinephrine, serotonin and dopamine in rat brain is described. The amines are acetylated and the derivatives are resolved by thin layer chromatography (TLC) on silica high performance thin layer chromatography (HPLC) plates. Quantification is achieved by fluorescence densitometry at 415 nm excitation wavelength.

Animals↗

PubMedDate not supplied

Thin-layer chromatographic determination of catecholamines, 5-hydroxytryptamine, and their metabolites in biological samples: a review.

A review of methodology for separation, detection, and quantitative determination of catecholamines, 5-hydroxytryptamine, and their acidic metabolites in biological tissue and fluids by thin-layer chromatography is presented. Selected procedures, including fluorometric scanning densitometry for catecholamine acetyl derivatives and color scanning densitometry for acids, are described.

Biogenic Amines↗