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Biomedical subjects

A Gaulier

Publications and source records attributed to A Gaulier.

At least 19 recordsLinked to original sources
PubMed1995

Systemic mastocytosis associated with chronic myelomonocytic leukemia: clinical features and response to interferon alfa therapy.

Systemic mastocytosis is a rare disease that shows marked heterogeneity in clinical manifestations and prognosis. It may be associated with hematologic disorders. We describe a patient with systemic mastocytosis associated with chronic myelomonocytic leukemia accompanied by ascites, pleural effusion, and development of skin lesions along a surgical scar. The disease responded well to interferon alfa therapy. This is the second report of successful treatment of mastocytosis with interferon alfa and the first associated with a hematologic malignancy.

Ascites↗
PubMed1995

[Lymphadenopathy of dermatosis in the course of drug hypersensitivity. Cytologic, immunohisto- and immunocytologic, ultrastructural aspects. Report of a case].

In addition to the morphological details obtained from the imprints, a simple immunocytological study allowed us to diagnose one case of a dermopathic lymphadenopathy simulating a T cell lymphoma, following a drug-induced erythrodermia. We were able to identify the increase of CD1a+ and Prot. S100+ cells on acetone fixed imprints. The histological, immunohistological and ultrastructural investigations confirmed the value of the cytological study and that the dendritic cells were Langerhans cells (Birbeck granules+). Most of them were considered as migrating from the dermal lesions.

Adolescent↗
PubMed1994

Bone marrow one step fixation-decalcification in Lowy FMA solution: an immunohistological and in situ hybridization study.

The immunoreactivity of paraffin embedded bone marrow biopsies (BMB) was studied following a one step 20-hour-fixation-decalcification in Lowy formalin mercuric chlorid acid solution which permits excellent histological stainings. Antibodies reactive with myeloid, megakaryocytic, erythroid cells, T and B lymphocytes, mastocytes and metastatic cells were compared. Nearly all antibodies working on paraffin sections were demonstrated on Lowy FMA fixed BMB. Special care was taken to define an optimal working dilution. Trypsinization was not necessary. A slide microwave pre-treatment appeared essential before testing CD20 L26, CD8, CD3, CD34, MB1 Kappa and Lambda antibodies. It was suitable for UCHL1, LN2, CD30 antibodies. The same fixative allowed an m RNA Kappa or Lambda in myeloma and EBER 1 EBV RNAs in HIV lymphoma visualization by in situ hybridization. The safety handling of the toxic mercuric chloride component is discussed.

Biopsy↗
PubMed1994

Coexistence of Meyerson's with Sutton's naevus after sunburn.

We described the simultaneous occurrence of perinaevic eczema (Meyerson's naevi) and Sutton's halo naevus in one patient. Characteristic clinical and histological features of perinaevic eczema were found around four benign melanocytic naevi on the limbs. In addition, the patient had a typical Sutton's naevus on the back. The association of Meyerson's naevi and Sutton's naevus has been reported only once in the past literature. Both diseases appeared a few weeks after a severe sunburn. The role of sun exposure in the development of eczematiform and/or vitiligoid reactions around melanocytic naevi is discussed.

Adult↗
PubMed1993

[Mesothelial cell. Cytology. Cytochemistry. Immunocytochemistry. Contribution of immunolabelling to the cytodiagnosis of serous effusions with cells of mesothelial aspect].

This paper reviews the contribution of cytochemical and immunocytochemical methods to the cytodiagnosis of serous fluids. For this purpose, 1000 fluids were reviewed (500 pleural and 500 peritoneal samples). The most reliable histochemical technique is the iron stain which labels macrophages. A minimum immunolabelling panel (including one or two epithelial markers and vimentin), applied to the identification of the usual phenotypes of reactive fluids with mesothelial cells or with macrophages, to adenocarcinomatous fluids and to malignant mesothelioma fluids, is proposed. The sensitivity and specificity of the reaction is discussed but, altogether the value of the method appeared rather limited as a correct diagnosis was obtained in 95% of cases by a careful morphological study.

Adenocarcinoma↗
PubMed1993

[Pancreatic insulinoma, adenomatosis of the Wirsung's duct and chronic pancreatitis. Apropos of a case].

The clinical investigations carried out in a 58 years woman complaining of malaise led to the discovery of an hypoglycaemia resulting from a secreting pancreatic insulinoma. In addition, a chronic pancreatitis, an endocrine hyperplasia (possible nesidioblastosis) and a villous adenomatosis of the pancreatic duct were diagnosed on two biopsies. The immunohistological tests performed on the insulinoma showed insulin, calcitonin and gastrin labelled cells. Electron microscopy displayed numerous neurosecretory granules. The peritumoral endocrine hyperplasia contained intermingled B, A and D cells respectively labelled by insulin, glucagon and somatostatin. Following the operation, the patient recovered without recurrence of the hypoglycaemia (three year follow-up). Factors which may explain such a rare pathological association are discussed.

Adenoma, Villous↗
PubMed1993

[Squamous erythema annulare centrifugum].

A case of squamous erythema annulare centrifugum has been observed. It was characterized by striking clinical features and peculiar histological findings, including necrosis of keratinocytes. A search for causative factors was negative. Treatment with tar ointment (Brocq) rapidly resulted in dramatic healing of the lesions; however, the usual features of annular psoriasis were absent in our case.

Erythema↗
PubMed1993

[Inclusion of microfilaments and conventional cytology. Two complementary techniques applied to products of puncture with fine needle].

A technique has been developed to complete the processing of fine needle aspiration biopsy material of deep and/or superficial organs: a method of collection and "prefixation staining" allowing visualisation of very small coherent fragments (microfragments) obtained by fine needle aspiration biopsy and which can consequently be submitted to histological examination. This method optimises CT-guided fine needle aspiration biopsies which, up until now, were only submitted to cytological examination (smears on slides, cell pellets, concentration and cytocentrifugation). Histological analysis of microfragments of 35 aspiration biopsies of various organs (pancreas, liver, adrenals, retroperitoneal nodes, paravertebral and mediastinal masses, etc.) was contributive in 71.4% of cases (25/35). In conclusion, the combination of cytological and microfragment inclusion techniques increased the diagnostic yield to 80% of cases.

Adenocarcinoma↗
PubMed1992

[Lipomas of the mesentery in young children].

Lipomas of the small bowel and mesentery are exceedingly rare in young pediatric patients. This article reports a case which underlines the difficulty of establishing this diagnosis before exploratory laparotomy.

Child, Preschool↗
PubMed1992

[Malignant intravascular lymphoma (malignant angioendotheliomatosis), with adrenal localization. Report of a case].

A past history of epilepsy and terminal dementia in a fifty five year old patient lead to the post mortem diagnosis of malignant intravascular lymphoma (malignant angioendotheliomatosis). There was a scattered involvement of capillaries and venulae in leptomeninges, peripancreatic and perirenal adipose tissue, pericardium, myocardium. The only adrenal involvement was typically tumoral at macroscopic examination. Such a localization, often reported in B intravascular malignant lymphoma, is studied according to the recent publications of this entity.

Adrenal Gland Neoplasms↗
PubMed1991

Do measles early giant cells result from fusion of non-infected cells? An immunohistochemical and in situ hybridization study in a case of morbillous appendicitis.

A case of acute appendicitis with features of measles inflammatory reaction is studied. Two types of lymphoid polykaryons are seen: Warthin Finkeldey cells inside germinal centres (LN1 ++, LN2 +, L26 +, MB1 +, MB2 +/-) and multinucleate plasma cells in the lamina propria (mu +, alpha ++, kappa +, lambda +). Both types of polykaryon are devoid of inclusions. The search for viral genetic information by in situ hybridization was negative in these cells. A positive signal was observed in interfollicular mononuclear cells and rare enterocytes. A possible mechanism of fusion from without, acting at the beginning of the disease to induce the appearance of polykaryons, is discussed.

Acute Disease↗
PubMed1990

Tamm Horsfall protein expression by a small renal cell carcinoma presenting with metastases.

The clinical presentation of cervical metastases in a young woman presenting with cervical lymphadenopathy is described. The clinical, histological, ultrastructural and immunological findings establishing the diagnosis of a renal primary tumour are seen. The significance of mixed tumour cell expression of antigens specific for the proximal tubule (CD10, DPP4 and aminopeptidase N) and of Tamm Horsfall protein, normally expressed on the thick ascending limb of loop of Henle and distal tubule, is discussed.

Adult↗