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Biomedical subjects

A Gennaro

Publications and source records attributed to A Gennaro.

6 recordsLinked to original sources

Recent developments in durum wheat chromosome engineering.

Transfer of alien chromosome segments from various Triticeae species into cultivated wheats, commonly referred to as "chromosome engineering", is currently benefiting from the recent, impressive advancements in molecular genetics, cytogenetics and genomics, which are providing new insights into the genetic and physical organization of even complex plant genomes, such as those of the Triticeae. The powerful analytical tools presently available are making the assessment of desired genotypes in the course of chromosome engineering far more precise and effective than in the past, thus giving this transfer strategy renewed and increased potential for meaningful practical achievements. Examples are given here of the application of such tools to the engineering of the durum wheat genome with small alien segments containing genes with beneficial impact on disease resistance and quality traits.

Chromosomes, Plant↗

Studies on the interaction between steffimycins and DNA.

The complexes formed between steffimycins and DNA were studied using various physicochemical techniques. The binding process has been followed spectrophotometrically or fluorimetrically. The binding parameters n and K, evaluated according to McGhee and Von Hippel, show a good affinity of these antibiotics for the macromolecule. Flow dichroism measurements showed that in the complex with DNA, the antracycline moiety of the steffimycins is intercalated between two base pairs of the macromolecule. The binding experiments with various polydeoxyribonucleotides and with various DNA samples, having different base pair compositions, suggest that an alternate sequence of A-T, such as that of poly[d(A-T)] . poly[d(A-T)], represents a good receptor site for the binding of steffimycins to DNA. The lack of in vivo activity of these antibiotics is discussed.

Anthracyclines↗

Splenomegaly with carcinoma of the colon and rectum.

Scans of the liver and spleen were reviewed for patients with carcinoma of the large intestine. The patients were divided into groups, depending upon the size of the liver and spleen. Those with enlarged spleens tended to have an increased survival time compared with those without splenomegaly. It is postulated that splenomegaly is a good predictor of survival when used with the Dukes' classification and that the increased size of the spleen is on an immunologic basis.

Adult↗

Photosensitized effects of furocoumarins: the possible role of singlet oxygen.

The capacity of various furocoumarins to generate singlet oxygen in aqueous solution has been determined. The antiproliferative and the skin-photosensitizing activities of the same furocoumarins did not show any correlation with the capacity to generate the singlet oxygen, while these photobiological properties could be correlated with the capacity of furocoumarins to induce photolesions to the DNA.

Animals↗