Binding of [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin to AH receptor was characterized in cytosol from human placentas in which the pregnancy outcome was normal compared with pregnancies in which there was some adverse outcome (premature birth; intrauterine growth retardation; structural abnormality). No significant difference was detected between normal and adverse outcomes in the concentration of AH receptor sites (Bmax) nor in the affinity with which [3H]TCDD bound to the receptor (Kd). Aryl hydrocarbon hydroxylase activity, a CYP1A1 enzyme regulated by the AH receptor, was elevated in placental microsomes from smokers; this elevation was associated with intrauterine growth retardation.

Aryl Hydrocarbon Hydroxylases↗

PubMedDate not supplied

Carbofuran: its toxicity to and metabolism by earthworm (Lumbricus terrestris).

Explore the source record for details and available documents.

Animals↗

PubMedDate not supplied

Dioxin-like compounds in fishing people from the Lower North Shore of the St. Lawrence River, Quebec, Canada.

In this study, investigators assessed exposure to dioxin-like compounds in a fishing population that inhabits small coastal communities along the Lower North Shore of the St. Lawrence River, Quebec. This population relies heavily on wildlife foods for sustenance. Investigators analyzed chemically the most popular marine foods (i.e., fish, crustaceans, sea mammals, and sea-bird eggs), and they also obtained 25 human plasma samples from individuals in two villages along the river. The mean level of total polychlorinated biphenyls in this population was approximately twice that found in the entire fishing cohort. Plasma levels of dioxin-like compounds, expressed as tetrachlorodibenzodioxin toxic equivalents, were approximately eight times higher than levels in urban residents. Most of the increase in tetrachlorodibenzodioxin toxic equivalents in the selected fish eaters resulted primarily from an elevation in polychlorinated biphenyls. Concentrations of dioxin-like compounds from the Lower North Shore were low in fish and seals, but concentrations were elevated in the eggs of sea birds. Given that there was also a significant statistical correlation in the entire population between human plasma levels and consumption of birds' eggs-and not other traditional foods-much of the increased human dose appeared to originate from this one food source. Because there appear to be increased, but uncertain, health risks from this elevated body burden, investigators advised the residents of the area to avoid consumption of wild birds' eggs (i.e., a food source of minor nutritional importance).

Adult↗

PubMedDate not supplied

Uranyl nitrate-induced proximal tubule alterations in rabbits: a quantitative analysis.

Naturally occurring uranium in drinking water is a significant health concern in several areas of North America. Because the kidney is a known target organ to examine the effects of uranium or its compounds, the objective of this study was to determine whether kidney repair occurs after exposure to, and withdrawal of, uranyl nitrate (UN). This work, part of a larger study to establish safe levels of uranium in drinking water supplies, examined the ultrastructural changes in proximal tubule cells of New Zealand white rabbits following subchronic exposure to UN in water and for 91 days after exposure ended. The rabbit was chosen as the experimental animal because of its high sensitivity to uranium. Animals were exposed to 24 or 600 mg UN per liter (UN/L) in drinking water for 91 days, with no recovery or recovery periods of 45 or 91 days. Ultrastructural changes, quantified by a stereological image analysis system based on point counting, were observed in renal proximal tubules (PTs). Each electron micrograph was statistically considered an experimental unit. The severity of lesions was directly proportional to the dose. Animals exposed to 600 mg UN/L had the most severe lesions; nevertheless, alterations were remarkable in animals exposed to the low dose. At both recovery periods, the lesions were significantly more severe than those in animals of the no-recovery group, which may result from the kidney's ability to store uranium. The PT cells had increased lysosomal and vacuolar mass as well as variations in mitochondrial mass. In addition, there was epithelial cell degeneration with a focal loss of brush borders, thickening and splitting of tubular basement membrane, and occasionally cell necrosis. Interstitial fibrosis of the renal cortex persisted as the recovery period increased in the animals of UN-dosed groups. Alterations may be due to disturbed fluid transport across the PT and other cells and decreased cell respiration resulting from damaged cell constituents. Cell damage caused by UN in drinking water persisted throughout the 91-day recovery period. By eventually determining the no observable effect level for the kidney by UN, this study may assist in devising a model to ascertain the safe levels of uranium in water.

Animals↗