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A Gombosová

Publications and source records attributed to A Gombosová.

17 recordsLinked to original sources
PubMed1992

Proteinases of Trichomonas vaginalis: antibody response in patients with urogenital trichomoniasis.

Immunoprecipitation combined with electrophoresis in gelatin-polyacrylamide gels was successfully used for detection of antibodies against numerous proteinases of Trichomonas vaginalis in infected patients. The method proved to be highly specific as anti-proteinase antibodies were absent in women with negative cultivation of T. vaginalis and no history of trichomoniasis. Sera of 71% and vaginal washes of 86% patients with trichomoniasis were positive for these antibodies. In vaginal washes, but not in sera, antibodies were partly complexed with proteinases, possibly of trichomonad origin. It was also shown that serum antibodies as well as local anti-proteinase antibodies persisted for weeks after patients had been cured.

Adult↗
PubMed1992

Vaginal leukocyte characteristics in urogenital trichomoniasis.

Morphological and functional characteristics of vaginal exudate leukocytes were examined in 47 patients with urogenital trichomoniasis. Electron microscopic morphology, viability, phagocytosis of Candida albicans blastospores and ability to undergo respiratory burst in the iodonitrotetrazolium reductase test were evaluated in these cells. Vaginal inflammatory leukocytes were almost exclusively polymorphonuclear neutrophils, and their concentration was positively correlated (r = 0.58; p less than 0.001) with the number of trichomonads in the exudate. Median leukocyte viability reached 39% and both phagocytic and tetrazolium reductase activities of these cells were significantly reduced in comparison with those of circulating polymorphonuclear leukocytes. Patients with a clinical picture of severe mucosal inflammation had significantly higher vaginal exudate leukocyte concentrations and viability than those without inflammatory signs. The possible role of vaginal leukocytes in the pathogenesis of urogenital trichomoniasis is discussed.

Adolescent↗
PubMed1992

[Super Duo latex fixation test--an alternative diagnostic method for trichomoniasis?].

The reliability of the new latex-agglutination test super duo for the detection of antigens of T.vaginalis and Candida, a prospective screening method for trichomoniasis, was verified. In a group of 27 women the microscopic and culture diagnosis of trichomoniasis was established in 22 patients and 21 of them were positive in the latex-agglutination test for T.vaginalis. The immunochemical examination was not positive in either of the five women with findings of negative cultivation for the parasite. The intensity of agglutination was in good correlation with the clinical signs of inflammation. In asymptomatic women the reaction was indistinct. Vaginal candidiasis was revealed by culture in two women, one of them was positive on the latex-agglutination test for Candida. One woman with negative cultivation finding for yeast microorganisms was positive in the immunochemical test. Examination of asymptomatic sexual partners of trichomoniasis patients would be necessary to evaluate the suitability of the set for T.vaginalis infection screening.

Adolescent↗
PubMed1990

Dot-immunobinding assay with monoclonal antibody for detection of Trichomonas vaginalis in clinical specimens.

A rapid and specific dot-enzyme immunoassay (DIBA) using monoclonal antibody was introduced for detection of Trichomonas vaginalis antigen in vaginal and urethral materials. The results of DIBA were compared with culture findings of the parasite in 245 female patients. Taking culture as the reference method, DIBA had a sensitivity of 92% and a specificity of 93%. The predictive value of a positive test was 98% and that of a negative test 77%. The efficacy of wet mount, culture and DIBA were compared in 134 women with trichomonal infection. The most sensitive method was cultivation with a positivity of 99.3%. DIBA was as sensitive as the wet mount (92%). In 51 sexual partners of women with trichomoniasis DIBA proved to be ineffective. The sensitivity of the assay with corpuscular antigen of T vaginalis was 1 x 10(4) cells/ml, with soluble antigen 47 micrograms/ml. Specificity of the assay was confirmed by lack of any cross-reactivity with Trichomonas tenax. Tritrichomonas mobilensis and Candida albicans.

Adolescent↗
PubMed1989

[Urogenital trichomoniasis and vaginal mycosis in female offenders--a repeat study 20 years later].

The prevalence of urogenital trichomoniasis and vaginal mycosis in 698 female prison inmates in Bratislava was compared with the occurrence of the diseases recorded twenty years ago. The occurrence rate of urogenital trichomoniasis (40.97%) was only slightly reduced compared to the sixties (59.17%), yet it was still five times higher than in the current population. The occurrence rate of vaginal mycosis is much lower in delinquent women (2.3%) than in other women. Trichomonas vaginalis was most frequently present in the age group of 20-29 years (44.67%). All patients with urogenital trichomoniasis were cured with nitroimidazoles. The first treatment was successful in 98.25%, the second treatment with the same dose had a 100% efficacy. Repeated examination of 84 women after two months did not confirm the possibility of intramural infection.

Adolescent↗
PubMed1988

Fewer Trichomonas vaginalis organisms in vaginas of infected women during menstruation.

The aims of the present study were to count Trichomonas vaginalis organisms recovered from the vaginas of patients with trichomoniasis, and to obtain data concerning changes in sizes of trichomonal populations during the menstrual cycle. In about 80% of symptomatic as well as symptomless patients, more than 1 x 10(5) parasites per ml could be obtained from vaginal washes. During menstruation, however, the number of trichomonads decreased appreciably, with subsequent increases within three to six days after bleeding. The results indicated that sufficient numbers of fresh trichomonads may be obtained from vaginal washes for biochemical and molecular experiments and also confirm the previously reported trichomonocidal effect of menstrual blood complement in vivo.

Adolescent↗
PubMed1988

Differential susceptibility of fresh Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus.

The ability of complement in human menstrual blood and cervical mucus to kill Trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. Only serum and menstrual blood with haemolytic activity produced total cytolysis of T vaginalis. The cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (EDTA), which confirms the role of complement in cytolysis. Most cervical mucus samples had no detectable trichomonal cytotoxic properties. The cytotoxic activity in the remaining samples was not due to complement, as it was heat stable. Fresh isolates of T vaginalis and subpopulations of fresh isolates differed in their susceptibility or resistance to complement mediated lysis in serum. Resistance to complement did not remain stable after trichomonads were grown in vitro.

Adolescent↗
PubMed1987

Phenotypes and protein-epitope phenotypic variation among fresh isolates of Trichomonas vaginalis.

Fresh isolates of Trichomonas vaginalis were examined for reactions to a panel of five monoclonal antibodies (MAbs). Four MAbs (C20A3, DM126, DM116, and C55) were to distinct surface immunogens and one MAb (L64) was to a cytoplasmic component. The fresh isolates were evaluated by indirect immunofluorescence (IF), immunoblotting, and radioimmunoprecipitation. IF assay with C20A3 MAb gave isolates which were homogeneous nonstaining (negative [Neg] phenotype) and isolates which were heterogeneous staining and nonstaining (positive [Pos] and Neg phenotype, respectively) organisms. Immunoblotting and radioimmunoprecipitation assays revealed that surface phenotypic heterogeneity among isolates with C20A3 MAb was due to the presence or absence of the immunogen from the parasite surface. IF assay with DM126 MAb also gave Pos and Neg phenotypes among parasites of some isolates. All of the isolates were always Neg phenotype with DM116 and C55 MAbs. The occurrence of Neg phenotype organisms with DM126, DM116, and C55 was due to epitope inaccessibility to their respective MAbs and not to the absence of the immunogen from trichomonal membranes. All isolates possessed the cytoplasmic protein recognized by L64 MAb. Paired isolates (taken 5 to 6 days apart) from 24 women were also studied. Four of the 24 paired isolates (16%) had different phenotype distributions at the two timepoints for C20A3. Fresh isolates also underwent phenotypic variation during in vitro growth and multiplication, as determined with C20A3. Also, 7 of the 24 paired isolates demonstrated dramatic changes in the accessibility of DM126 MAb to epitope binding. Lastly, 55 (90%) of 60 serum samples from patients with trichomoniasis evaluated in this study possessed antibody to the C20A3 reactive molecule. The data show that the fresh T. vaginalis isolates were predominantly Neg phenotype and confirm the occurrence of protein and epitope phenotypic variation for major immunogens among fresh isolates of the pathogenic human trichomonads.

Antigens, Protozoan↗
PubMed1986

Immunotherapeutic effect of the lactobacillus vaccine, Solco Trichovac, in trichomoniasis is not mediated by antibodies cross reacting with Trichomonas vaginalis.

According to the producers of the lactobacillus vaccine, Solco Trichovac, its therapeutic effect in trichomoniasis is achieved by antibodies that are induced by the vaccination and cross react with Trichomonas vaginalis. Common antigens of Lactobacillus acidophilus from Solco Trichovac vaccine and T vaginalis were therefore sought by three different seroreactions. Immune serum against Lacidophilus obtained by vaccinating two healthy human volunteers and two rabbits with the original Solco Trichovac vaccine, as well as hyperimmune rabbit antiserum to T vaginalis, were tested with each of the two micro-organisms. No evidence of antigenic similarity between L acidophilus and T vaginalis was obtained with either serum in any of the three serological tests. A non-specific immunostimulatory effect therefore seems to be a more probable explanation of the mode of action of Solco Trichovac vaccine.

Adult↗
PubMed1982

Dialysable specific transfer factor in mice immunized with attenuated Langat virus from the tick-borne encephalitis complex: generation, action and quantitative assay.

Cytolytic T lymphocyte assay was developed in order to measure the response of inbred C3H mice to dialysable specific transfer factor (STF), induced in subadult outbred mice by one shot immunization with the attenuated Langat virus. The first STF activity in mice splenic leukocytes was detected between 48-72 hr after virus administration. The conversion of splenic T-cell cytotoxic response in C3H mice in vivo occurred between 15-21 hr after STF administration. The killing activity of T-cells, induced by STF, showed cross-reactive traits within the genus Flavivirus. STF, given prior to the live virus, augmented the specific cytolytic T-cell response. In the live virus-primed mice the booster effect was markedly enhanced when administration of STF preceded the second immunization dose. In the serum of STF recipients, interferon was irregularly detected attaining low levels for short time periods. Temperature of 56 degrees C for 60 min abolished the activity of least 10(4) murine STF units, temperature of 37 degrees C lowered after 24 hr this activity by 3 log10units. Chromatography of the dialyzed leukocyte lysate on Sephadex G-25 column yielded usually five peaks. The second peak showed an increased content of ribose-bound and protein materials and, as a rule, a relatively concentrated STF activity.

Animals↗