The efficacy of the commercially available Vacutainer blood culture system to support the growth of non-sporing anaerobes was compared with two laboratory-prepared blood culture media (supplemented thioglycollate medium and brain heart infusion). The media were inoculated with 10, 100 and 1000 organisms of the species tested, and the number of colony-forming units was determined at intervals of 8-10 hrs. Analogous experiments were performed with batches of the media to which 10% vol/vol of human blood had been added. From the results obtained with strains of Bacteroides fragilis, Bacteroides thetaiotaomicron, Bacteroides splanchnicus, Sphaerophorus necrophorus, Peptostreptococcus anaerobius and Propionibacterium acnes it became obvious that growth of non-sporing anaerobes was significantly enhanced in the Vacutainer culture tubes, whereas Enterobacteriaceae and Streptococcus spp. grew equally well in all the media tested.

Bacteroidaceae↗

PubMed1971

[Guidelines for obtaining cadaver blood for alcohol determination].

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Cadaver↗

PubMed1971

[Intrathoracic expansive process].

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Humans↗