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A Gryszkiewicz

Publications and source records attributed to A Gryszkiewicz.

5 recordsLinked to original sources
PubMed1976

[Erythrocyte freezing in the presence of hydroxyethyl starch].

Biochemical properties of erythrocytes frozen in the presence of hydroxyethyl starch (HES) as preserving substance produced in this country were assessed. Erythrocytes were frozen adding HES in 4 different concentrations. The most favourable results were obtained with erythrocytes subjected to the action of HES in 30% and 35% concentrations. The erythrocyte 2,3-DPG level during freezing with 35% HES was within normal limits while it decreased by 10% after defreezing when 30% HES had been used. The ATP concentration in erythrocytes fell by 50% in relation to the initial value. A slight fall of P50 was observed and erythrocyte loss after defreezing did not exceed 10%. The 35% HES solution in a 0.15 mol/l sodium chloride solution was most favourable for freezing erythrocytes in liquid nitrogen. A simple method of preparation of defrozen erythrocytes for transfusion was elaborated.

Adenosine Triphosphate↗
PubMedDate not supplied

[Use of hydroxyethyl starch as a cryoprotective medium during platelet storage at low temperatures].

The effects of HES and HES + DMSO used as cryoprotective media for storage of human platelets in liquid nitrogen and vapor phase of liquid nitrogen were studied. Solution of 6% and 15% HES with molecular weight ranging from 65,000 to 250,000, and 10% DMSO were used. The criteria accepted for evaluation of the efficiency of these cryoprotective media were: 1. platelet counts, 2. participation of platelets in the processes of hemostasis measured in vitro by the ability of platelets to release adenine nucleotides (ATP + ADP) after thrombin stimulation. It was found that 15% HES is a more effective cryoprotective medium than 6% HES. The use of 15% HES + 10% DMSO gave similar results as the use of 10% DMSO alone.

Blood Cell Count↗
PubMedDate not supplied

[Fate of C-14 labeled hydroxyethyl starch in mice].

Three preparations of hydroxyethyl starch having MS 0.55, 0.65 and 0.8 respectively and labelled with 14C in hydroxyethyl residue were administered to mice. After 1, 5, 10, 20 and 60 days the mice were sacrificed and radioactivities determined in the following organs and tissues: blood, spleen, liver, kidneys, heart, lungs and muscles. Most of the initial dose of radioactivity was eliminated from mice within 24 h. The remaining portion was eliminated slowly and even after 60 days 0 07--1.2% of the initial dose could be detected in different organs. The rate of clearance of radioactivity was fastest for hydroxyethyl starch with MS = 0.55 and slowest for the preparation with MS = 0.8. The retention of radioactivity was most conspicous in the muscles.

Animals↗
PubMedDate not supplied

[Polish hydroxyethyl starch preparation: a clinical study].

The 6% solution of hydroxyethyl starch (MS = 0.47-0.7, Mw = 120,000-220,000) prepared at the Department of Biochemistry, Institute of Haematology in Warsaw was administered in infusions to a group of surgical patients. It was found that the preparation complied with the conditions and requirements for plasma-replacing agents. In none of the treated patients any side effects were observed. Further investigations will be continued infusing greater volumes of HES to patients with hypovolaemia of different origin.

Adult↗