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A H Band

Publications and source records attributed to A H Band.

11 recordsLinked to original sources
PubMed1986

Mechanism of phagocytosis of mycobacteria by Schwann cells and their comparison with macrophages.

Factors influencing the phagocytosis of mycobacteria by 33B rat Schwannoma cells and rat peritoneal macrophages were studied. Uptake of 14C-acetate-labeled Mycobacterium w by these cells was used to set up a radiometric phagocytosis assay. Incubation at 4 degrees C and treatment with sodium azide (0.2% to 1%), colchicine (10(-7) to 10(-3) M), cytochalasin B (0.2 micrograms/ml to 25 micrograms/ml), and dibutyryl cyclic AMP (10(-7) to 10(-3) M) inhibited the phagocytosis by both cell types in a similar manner. These experiments demonstrate similarities in the mechanism of phagocytosis of mycobacteria by Schwann cells and macrophages.

Animals↗
PubMed1986

Lack of Mycobacterium leprae-specific uptake in Schwann cells.

Among mycobacteria, Mycobacterium leprae have a unique property to infect peripheral nerves, which is the cause of a variety of debilities seen in leprosy. The possibility of selective uptake of M. leprae by Schwann cells was studied using a rat Schwannoma cell line 33B and rat sciatic nerve-derived Schwann cells. M. leprae were phagocytosed by 33B cells but so also were seven other mycobacteria ("Mycobacterium w," BCG, M. tuberculosis H37Rv, M. nonchromogenicum, M. vaccae, ICRC bacillus, and M. smegmatis) which do not involve peripheral nerves. All three mycobacteria tested (M. leprae, M. tuberculosis and "Mycobacterium w") were phagocytosed by sciatic nerve-derived Schwann cells. Both Schwannoma and Schwann cells phagocytosed even inert latex particles. These results fail to demonstrate any M. leprae-specific uptake system in Schwann cells.

Adhesiveness↗
PubMed1985

A dot enzyme-linked immunosorbent assay for detection of antibodies against Entamoeba histolytica.

A visual micro-dot enzyme-linked immunosorbent assay (ELISA) based on detection of antibodies against soluble antigens of axenically grown cultures of Entamoeba histolytica is described. The antigen was spotted on a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (BSA) and incubated with 3-fold dilutions of patient sera followed by incubation with protein A conjugated to peroxidase. Enzymic activity was evidenced using the substrate 4-chloro-1-naphthol. A positive reaction produced a blue spot. The sensitivity of the assay was better and comparable to the indirect haemagglutination assay (IHA) and plate ELISA. The entire assay could be completed within 3 h. Antigen-loaded and pre-blocked nitrocellulose strips could be stored up to 3 months at room temperature and 37 degrees C.

Amebiasis↗
PubMed1985

Production of prostacyclin by different cell types of the goat ovary.

A sensitive platelet aggregation-inhibition assay was used to quantitate the production of prostacyclin by different cell types of the goat ovary. The assay could detect as low as 0.16 ng in the test sample. Different cell types i.e. granulosa, theca and corpus luteum or the total ovarian homogenate were incubated at 37 degrees C for 10 minutes with or without 0.2mM arachidonic acid. Rat aortic strips were incubated under similar conditions as a positive control. Under basal conditions the amount of prostacyclin produced by corpus luteum cells was higher compared to that by granulosa cells. When the precursor of prostaglandins (arachidonic acid) was provided the production markedly increased in corpus luteum, granulosa, and ovarian homogenate as well as in aortic strips. Theca cells did not produce detectable levels of prostacyclin even when the precursor was provided. Trapidil did not alter the basal but enhanced the arachidonic acid-stimulated prostacyclin production in homogenate and granulosa cells with no further increase in corpus luteum cells. U-51605 decreased basal as well as arachidonic acid-stimulated prostacyclin production in all the cell types. The prostacyclin production in ovaries is compartmentalized suggesting a possible role in ovarian physiology.

Animals↗
PubMed1984

Behcet syndrome.

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Behcet Syndrome↗
PubMedDate not supplied

Characterization of antibodies to a placenta-specific antigen cross-reacting with a choriocarcinoma cell line.

Antibodies were raised in rabbits to purified human term placental villous plasma membrane. These were cytotoxic to human peripheral blood lymphocytes and manifested cross-reactivity to kidney and liver. After absorption with these tissues, reactivity was retained with placental villous plasma membrane. The placental-specific antiserum was fully reactive with BeWo choriocarcinoma cells, but was devoid of reaction with a human myeloma tumor cell line (GM2132). The antibodies were cytotoxic to BeWo in the presence of complement, as determined by trypan blue uptake, 51Cr release, and inhibition of uptake of 3H-thymidine.

Animals↗
PubMedDate not supplied

A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-I in sera from leprosy patients.

A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.

Animals↗