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Biomedical subjects

A H Goldstone

Publications and source records attributed to A H Goldstone.

15 recordsLinked to original sources
PubMed1979

Acute promyelocytic leukaemia: further evidence of the differentiation-linked expression of Ia-like (p28,33) antigens on leukaemic cells.

Patients with acute non-lymphoblastic leukaemia were studied to determine if the presence of Ia-like antigens on leukaemic cells was related to the maturity of a leukaemic cell line. Ia-like antigens were present in the majority of acute myeloblastic and myelomoblastic leukaemias. 4 patients with acute promyelocytic leukaemia were Ia-negative. This finding suggests that Ia-like antigens reflect normal differentiation linked events in haemopoiesis. The presence of Ia-like antigens on leukaemic cells did not alter the patient's response to chemotherapy.

Adolescent↗
PubMed1979

Clonal identification in acute lymphoblastic leukemia.

In a case of acute lymphoblastic leukemia, two distinct types of leukemia blast cells could be identified throughout the course of the disease. The initially dominant type of blast cell was sensitive to chemotherapy; the other was drug-resistant, gradually becoming dominant as the disease progressed. The cell types could be clearly separated by their morphologic and surface membrane marker characteristics. The same chromosomal constitution was present in both types of blast cells, indicating a common clonal origin. Additional chromosomal abnormalities were present in the later stages of the disease, demonstrating that a distinct subclone had proliferated. This study illustrates that in some cases of acute leukemia, disease relapse is caused by growth of drug-resistant subclones that may be clearly identified by changes in morphology and surface membrane marker characteristics.

Adult↗
PubMed1977

Membrane marker analysis of 'lymphoid' and myeloid blast crisis in PH1 positive (chronic myeloid) leukemia.

The membrane phenotype of leukaemic cells was analysed during different stages of chronic myeloid leukaemia by a panel of markers. These included antisera against ALL antigen, p23,30 (Ia-like structure) and other T cell, B cell and myeloid markers 'Lymphoid' blast crisis shares the phenotype of common ALL (of non-T, non-B variety). Both leukemias react with anti-ALL serum and have pre-myeloid, pre-B lymphoid and pre-thymocyte characteristics. Their phenotype may reflect the characteristics of the pluripotential stem cell from which they derive. Nevertheless both leukaemias retain their undifferentiated characteristics and lack overt myeloid, B cell and thymocyte differentiation markers. Myeloid blast crisis and AML are negative with anti-ALL serum but some of the poorly differentiated myeloblasts react with anti-p23,30 serum (and negative for SmIg). The anti-p23,30 serum (used in a double marker assay combined with anti-immunoglobulin) detects some (4-11%) intermediate sized agranular p23,30+/SmIg-cells in peripheral blood during the chronic phase of CML as well as in normal foetal bone marrow. These could be myeloid stem cells (from which in CML the myeloid blast crisis arises). The results demonstrate that surface membrane analysis can aid exact diagnosis in different stages of CML.

Antigens, Neoplasm↗
PubMed1977

ATYPICAL SURFACE MAKER CHARACTERISTICS IN A T-cell lymphoma.

A case of T-cell lymphoma with an unusual phenotype is described. The majority of malignant tumour cells did not form E-rosettes, and lacked surface immunoglobulin but reacted with an antiserum to T cells and thymocytes. Localised acid phosphatase and non-specific alpha naphthol acid esterase activities and a prominent, convoluted, nuclear pattern in some neoplastic cells also supported the T cell derivation. The results demonstrate the importance of using a panel of markers for identification of the cellular derivation of some lymphomas.

Acid Phosphatase↗
PubMed1976

A case of small-cell Sézary's syndrome with null-cell features.

A case of the small-cell variant of Sézary's syndrome (SS) is reported in which the SS cells lacked the surface-marker characteristics of both T- and B-cells. In particular, the SS cells failed to form E-rosettes even with a sensitive technique using 2-amino-ethylisothiouronium bromide (AET)-treated sheep red blood cells. The significance of these findings is briefly considered in relation to the existing literature.

Aged↗
PubMed1976

HLA antigens in pernicious anaemia.

The increased frequency of HLA-B7 alone and HLA-A3/B7 together, in the same patient, has been confirmed in pernicious anaemia. There is no increase prevalence of HLA-A3 alone. No association has been found between the presence of serum intrinsic factor type 1 antibody and HLA-A3 or HLA-B7.

Anemia, Pernicious↗
PubMed1976

IgA and IgM cytoplastic inclusions in a series of cases of chronic lymphocytic leukaemia.

Seventy-two cases of typical chronic lymphocytic leukaemia were screened by electron microscopy for the presence of intracytoplasmic immunoglobulin crystals. Immunoglobulin inclusions were found in four cases. Immunofluorescent studies showed that the inclusions contained IgA in two cases and IgM in the other two patients. Lambda light chain specificity was demonstrated in all four cases. The ultrastructure of the inclusions was identical in each patient except that in one of the IgA cases the inclusions were found in the perinuclear cistern in addition to the more usual location within cisternae of rough endoplasmic reticulum. Surface immunofluorescence showed mu heavy chains in the two cases displaying IgM crystal formation, but in the two IgA patients, no alpha heavy chains were demonstrable at the cell surface. The possible significance of these findings is discussed in relation to the existing literature.

Cytoplasm↗